D_Purchase Description.pdf

PURCHASE DESCRIPTION

1. Statement of Need and Purpose: This request is for the purchase of TSQ Altis Plus Triple Stage Quadrupole Mass Spectrometry equipment for the iPSC Neurodegenerative Diseases Initiative (INDI) Project within the new Center for Alzheimer’s Disease and Related Dementias (CARD).

2. Background Information and Objective: As part of the iPSC Neurodegenerative Disease Initiative, NIA plans to measure how neurodegeneration-related mutations alter protein networks of disease-relevant iPSC-derived cells. The most sensitive and scalable method to measure these changes is through proteomic mass spectrometry. Recent advances in mass spectrometer technologies, particularly in orbitrap-based mass spectrometers, have enabled label-free data independent acquisition based quantification of thousands of proteins, including low-abundant proteins, from biological samples in single runs.

3. Generic Name of Product: Mass Spectrometer

4. Purchase Description:

Description: Cat # Quantity

TSQ ALTIS PLUS TSQ03-10002 1.00

KIT, PRE-INSTALLATION, TSQ2 OPTON-30666 1.00

POWERVAR UPS SPII 6.0kVA, RoHS C0012-01-00077 1.00

PV PDU 4x 6-20R, 4x 5-15R, NOT EU C0202-99-00181 1.00

CMPTR,HP,Z8G4T, 16-CORE,WIN10PRO64,RoHS OPTON-30850 2.00

EASY-SPRAY SOURCE KIT (NG) ES082 1.00

MONITOR,HP,27-IN,4KUHD,RoHS OPTON-30852 6.00

3 DAYS LSMS ONSITE SPPRT 702-022409 2.00

VANQUISH NEO SYSTEM VN-S10-A-01 1.00

VANQUISH DISPLAY 6036.1180 1.00

COLUMN COMPARTMENT N VN-C10-A-01 1.00

VALVE 2P-6P, LOW-DISP, 6250.1520 1.00

VIPER PLUG TITANIUM 6040.2303 1.00

ION BENCH WITH STACK MOUNTING KIT 6036.1720 1.00

POWER CORD USA-VERSION 6000.1001 4.00

CYTOCHROME C DIGEST, 1.6NMOL, LYOPHILZE 161089 1.00

Description: Cat # Quantity

VANQUISH NEO SYSTEM VN-S10-A-01 1.00

VANQUISH DISPLAY 6036.1180 1.00

COLUMN COMPARTMENT N VN-C10-A-01 1.00

VALVE 2P-6P, LOW-DISP, LOW-DISP, 150 MPA, BIO, VN-C 6250.1520 1.00

CYTOCHROME C DIGEST, 1.6NMOL, LYOPHILIZE 161089 1.00

EASY-SPRAY PEPMAP NEO C18 2UM 75UMx150MM ES75150PN 2.00

POWER CORD USA-VERSION 6000.1001 4.00

VIPER PLUG TITANIUM 6040.2303 1.00

FAIMS PRO DUO FMS03-10001 1.00

SII FOR XCALIBUR: DVD, CURRENT VERSION 4780.0384 1.00

SW, PROTEOME DISCOVERER 2.5 OPTON-31040 1.00

WIN 7 TO WIN 10 MIGRATION BUNDLE, LCMS 701-057882 1.00

CAP EASY 15um EMITTER w/o LINE ES994 1.00

ATLIS ION BENCH 278-1023000 1.00

Salient characteristics All deliverables required under this contract shall be packaged, marked and shipped in accordance with Government specifications. At a minimum, all deliverables shall be marked with the contract number and Contractor name. The Contractor shall guarantee that all required materials shall be delivered in immediate usable and acceptable condition.

Vendors must be able to provide these specifications:

A. General

• The system must have a footprint of not more than (w x d): 450 mm x 650 mm.

• The system must have a touch interface that allows for diagnostic instrument control.

• The system must have a pressure range reaching to 1500 bar (21,750psi).

• The system must be able to run gradients at flow rates from 100 nL/min to 100

(21,750psi).

• The system must provide an iron free flow path.

• System gradient delay volume must be < 0.5μL in nano/Cap configuration and < 2μL in micro-flow configuration.

• The system must have an unlock-to-slide system base.

• Each module must be exchangeable without uninstalling the system for easiest maintenance.

B. Pump

• The pump must not require hardware changes to cover the entire flow range specified.

• The pump must have a settable flow range from 1 nL/min to 100 μL/min in 1 nL increments.

• The pump must have active flow control from 1 nL/min to 100 μL/min.

• The pump gradient delay volume contribution must be < 25 nL.

• The pump must be pre-calibrated for usage with common solvents.

• The system must provide a retention time precision of ≤ 0.2% RSD or ≤ 0.1 SD min, whichever is greater for TGQAPGFSYTDANK Cytochrome C protein digest peptide based on the installation qualification procedure.

C. Sampler

• The sampler must be able to thermostat samples between 4 and 40°C and stable within +/- 1K.

• The sampler must provide automated barcode recognition of the type of sample rack or well plate and detection of empty sample segments.

• The injection principle of the autosampler must be in-line split-loop (also called flow through needle) for high reproducibility injections with no sample loss.

• The sampler must support a sample capacity of four sample racks with any combination of the following types: 54 × 12 mm OD vials (≤ 1.5 mL), 96 × 6, 7 and 8 mm OD vials (≤ 1.2 mL), 16 × 15 mm OD vials (≤ 4 mL), 9 × 22.5 mm OD vials (≤ 10 mL), and well plates (96 and 384, deep and shallow) without the need for external devices such as plate feeder.

• The sampler must offer up to 4 independent wash liquids: outer and inner needle wash, weak and strong each.

• The sampler must offer sample loop and inner needle rinsing with two independent wash liquids.

• The sampler must offer dip rinse and continuous rinse of outer needle surface with two independent wash liquids.

• The sampler must be able to inject 2.5 μL from 3 μL in a high recovery vial using vial bottom detection.

• The sampler must have active pressure monitoring for injection to allow for minimal column shock upon injection.

• The sampler should be able to offer a standard injection volume range of 0.01–25 μL and extendable to 100 μL, settable in 0.01 μL increments. The injection volume range must be extendable for trap-and-elute workflows, i.e., high-volume trapping, by use of a larger sample loop and by multiple draw-and-trap cycles up to 500 μL.

• The sampler must allow forward-flush and back-flush trap-and-elute injections without fluidics exchange.

• The autosampler must be able to fulfill the following injection precision requirements for repeatable analysis:

o From 0.05 to 0.20 μL: ≤ 5.0% RSD o From 0.20 to 0.50 μL: ≤ 1.5% RSD o From 0.50 to 2.00 μL: ≤ 0.5% RSD o Above 2 μL: ≤ 0.25% RSD

• Injection accuracy must fall within +/- 0.5% for a typical aqueous sample injection of

5 μL.

D. Column Compartment

• It must be possible to equip/upgrade the system with a thermostatted column compartment.

• The column compartment must hold up to two valves.

• The column compartment must support heated trap-and-elute workflows.

• The column compartment must heat columns up to 80 °C.

• The column compartment must come with clips that secure the analytical column(s).

• The column compartment temperature precision must be within +/-0.1 K.

• The column compartment temperature accuracy at 50 °C must be within +/- 0.5.

• Column switching valves must be: suitable for low-flow LC, i.e., valves with a swept volume less than 60 nL port-to-port.

o Able to withstand long-term use at 1500 bar backpressure.

o Able to withstand long-term use at a temperature of at least 60 °C.

o Grounded to ensure electrical avoiding charge build up from HV ion sources.

• The column compartment must fit columns up to 300 mm.

• Passive preheater must be available for use with the column compartment.

E. Vanquish User Interface

• The user interface must display the status of all supported devices through a graphical, multilanguage user interface.

• The user interface must be a glove-enabled and solvent resistant touch display.

• The user interface must allow direct instrument control without any PC connection.

• The user interface must allow remote instrument control.

• The user interface must provide maintenance and service support through status feedback and media content.

• The user interface must provide automated procedures for instrument operation and diagnostics.

• The user interface must provide guided workflow exchange and maintenance procedures.

F. LC Method Editor

• The system must allow creating solvent gradients by time, total volume, or number of column volumes.

• The system must consider column dimensions for adaptive calculation of column re-equilibration times.

• The system must prevent operating outside of column temperature, flow, and pressure specifications.

• The system must allow for flow/pressure or combined controlled acceleration of sample loading and column re-equilibration.

G. Software Control

• The system must be able to run under a Thermo Scientific™ Xcalibur™ software based data system for instrument control and acquisition.

• The system must support Thermo Scientific™ Chromeleon™ Chromatography

Data System (CDS) software.

5. Delivery Date: Six (6) months after receipt of purchase order