D-Purchase Description.pdf

Version 11-30-2016-1 FAR Part 11—Describing Agency Needs

PURCHASE DESCRIPTION

1. Statement of Need and Purpose: To acquire consumables for spatial transcriptomics, enabling the identification of age-related changes in gene expression at single-cell in our circadian studies. This advanced technology, compatible with single nucleus RNA-sequencing, will pinpoint anatomical locations of aging cells within tissues. Such precision is essential for identifying aging focal points and interventions for longevity projects at the National Institute on Aging (NIA), specifically within the Circadian Biology of Aging Unit (CBAU).

2. Background Information and Objective: The National Institute on Aging requires consumables for spatial transcriptomics to support ongoing and future research on controlled dietary interventions and circadian rhythms in aging mice. The Seeker Kit Bundle represents an advanced technology in spatial sequencing, enabling the analysis of gene expression within intact tissue samples while maintaining their spatial context. This innovative kit integrates high-resolution imaging and sequencing technologies seamlessly. Initially, tissue samples are prepared on a specialized platform that preserves spatial information, capturing the precise location of each cell through imaging. Following preparation, the samples undergo sequencing where RNA molecules are converted into cDNA, amplified, and then sequenced to quantify gene expression levels in each cell. Importantly, spatial information acquired during imaging persists throughout sequencing, enabling the reconstruction of cellular spatial organization. This capability provides critical insights into spatial variations in gene expression across tissue regions, essential for understanding circadian and longitudinal aging dynamics. The Seeker Kit Bundle also includes computational tools and algorithms crucial for aligning sequencing data with spatial information accurately, thereby facilitating the interpretation of complex spatial transcriptomic datasets

3. Generic Name of Product: Spatial Transcriptomics Seeker Bundle Kit

4. Purchase Description:

Hardware:

● (Quantity 6) 10 x 10 Trekker U Kit Bundle.

Other:

● (Quantity 1) Trekker training kit bundle

● (Quantity 1) Dry Ice Shipping

5. Salient characteristics:

Products:

● 10x10 Trekker U Kit Bundle, Early Access, 4 Tiles, SK010

● Trekker Training Kit Bundle, SK008

Version 11-30-2016-1 FAR Part 11—Describing Agency Needs

● Curio Trekker UV Lamp Holder, ME002

Key Features and Justification:

Unique Technology:

● Curio Bioscience has commercialized the Curio Trekker, a reagent kit based on the

Slide-Tags technology from the Broad Institute, Cambridge, MA.

● Technology recently developed and validated: Russell, A.J.C., Weir, J.A., Nadaf, N.M. et

al. Slide-tags enables single-nucleus barcoding for multimodal spatial genomics. Nature 625, 101–109 (2024). https://doi.org/10.1038/s41586-023-06837-4

● Curio Bioscience has made exclusive improvements to the Slide-Tags technology, making them the sole supplier.

● Compatible with snRNA (single nucleus RNA sequencing, using 10x standard pipeline)

● The Curio Trekker kit is the only product that provides unbiased whole-transcriptome spatial data at true single-cell resolution in fresh frozen tissue.

Comprehensive Kit Components:

● The kit includes Tiles and reagents to barcode single nuclei from fresh frozen tissue, facilitating upstream single nuclei sequencing.

● Nuclei from fresh frozen tissue sections are tagged with spatial barcode oligonucleotides from DNA-barcoded beads positioned on the Tile.

● These tagged nuclei are then analyzed with existing single-cell methods, incorporating spatial coordinates.

Advanced Data Processing:

● Sequencing data obtained is processed through the Curio Trekker Pipeline to map and visualize nuclei locations on the Tile.

6. Quantity:

● (Quantity 6) SK010, 10 x 10 Trekker U Kit Bundle;

● (Quantity 1) SK008 Trekker training kit bundle;

● (Quantity 1) Vendor Dry Ice Shipping Charges.

7. Delivery Date/Location:

All items shall be delivered within 8 weeks after receipt of Purchase Order to:

National Institute on Aging, TGB, CBAU 251 Bayview Blvd, BRC Room 05B129 Baltimore MD 21224