D Purchase Description.pdf

Version 11-30-2016-1 FAR Part 11—Describing Agency Needs

PURCHASE DESCRIPTION

1. Statement of Need and Purpose:

The study of vascular function is central to understanding neurovascular biology. We need fast in vivo measurements to investigate vascular structure and function of cerebral vasculature. We require two photons with multi wavelength dual channels imaging system to study these functions of cerebral vascular in vivo with the ability of image and stimulate multiple vascular and neuronal cells simultaneously in different imaging planes.

To achieve this, our laboratory requires a versatile, hands-free and fully integrated ultra-fast femtosecond laser system, with a complete tuning range of 660-1320nm.

Furthermore, due to the nature of our multi-color experiments, the system needs to provide a second, fixed wavelength output at 1040nm which is phase-locked to the tunable beam for imaging.

We also require a single high-power output laser capable of greater than 40W average power at 1035nm to stimulate a field of view at multiple depth using spatial light modulator (SLM) unit. The system should provide fully variable repetition rates, variable pulse length and integrated modulation capabilities via AOMs to provide pulse picking and repetition rate down selection.

2. Background Information and Objective:

One of the goals in the Neurovascular Research Unit is to identify function of neurovascular units in different brain regions in vivo in physiology and models of vascular cognitive impairment and dementia (VCID) including stroke. In order to conduct these experiments, we heavily rely on a two-photon microscope with a tunable and a fixed wavelength laser. The tunable laser with wavelength range of 660-1320 nm allows use of multiple fluorophores in the imaging set-up for simultaneous measurements of functional activity in different cell types in vivo (i.e. neurons, vascular endothelial cells, mural cells etc.). The use of a high output (>40W) with fixed output (1035 nm) with the use of SLM will allow us to perform selective and localized optical stimulation of different neurovascular cells to evaluate specific functions of individual cells with spatiotemporal resolution.

3. Generic Name of Product: - Multi-wavelength Dual laser for multi-color imaging system

- Fixed-wavelength laser for optical stimulation

4. Purchase Description:

BRAND NAME ONLY OF THE FOLLOWING:

Part Number Description Qty

1385777 Chameleon Discovery NX with TPC: Dual 1 2264706 Laser Head: Monaco LX 1035-40-40-C: Series I: Scientific 1

1446050 SMC Chiller Package: Air Cooled: Large Bypass: Rack Mount: 1.2kW: 100-115VAC Single Phase: HRR Series 1

2219597 Post Kits for 4.75" Beam Height with 1/4"-20 / M6x1.0 Thread Setscrews: Monaco (Series II) 1

1305895 Productivity Plus Gold - Chameleon Discovery Extended Warranty 1

5. Salient characteristics

Multi-wavelength Dual laser for multi-color imaging system.

Sealed Computer-controlled Integrated ultrafast laser system with following performance and Capabilities:

1. System

• Sealed, contamination free ultrafast cavity with no requirement for external course mirror adjustment from external ports.

• Closed loop water cooling, with a Peltier-driven cooling engine for minimal acoustic noise.

• Simple integration – the system must be user-controllable either via RS232 or via the vendor-supplied GUI.

• Advance Replacement service strategy to minimize user down time, supported by a remote service facility.

• 80MHz Synchronization output built in for triggering detection systems.

• Laser head footprint <0.4m2 to optimize optical table space.

• Operating temperature range 15-35oC

• HALT designed, HASS tested for highest reliability and long lifetime

• Fully integrated power modulation of both outputs via fast AOM control

2. Tunable Beam

• Tuning range of 660nm to 1320nm

• Average power >1.4W at 700nm, >2.7W at 800nm, >2.6W at 900nm, >2.15W at

1000nm, >1.85W at 1200nm, >1.2W at 1300nm

• Tuning speed >50 nm/s averaged across 660-1320nm

• Beam quality to be specified as TEM00 to ensure a tight focal volume, increasing excitation and enhancing resolution.

• Power stability of +/-1% after 2-hour warm-up and <+/-1oC ambient change

• Pulse duration 100fs at 900nm (sech2 pulse shape).

• Automated dispersion compensation on the tunable output (after AOM) of 0 to -

40,000fs2 at 680nm, 0 to -17,000fs2 at 800nm, 0 to -5000fs2 at 1050nm, and 0 to - 4000fs2 at 1300nm

3. Fixed Wavelength Beam

• Average power >3.5W at 1040nm

• Pulse duration 140fs (sech2 pulse shape)

• Power stability +/- 1%

• Noise <0.25% rms in a 10Hz-10MHz band

Fixed-wavelength laser for optical stimulation.

• One-box, hands-free, turn-key amplified fiber laser system with computer control and software GUI. The sealed box system has no additional requirement for external mirror adjustment from external ports. The laser has following unique specification:

• Fundamental: 40W, 40uJ, 1MHz Options: (40W, 20uJ, 2MHz), (40W, 10uJ, 4MHz), etc.

• Beam quality to be specified as o TEM00 with M2 < 1.2 o Beam circularity >85%

• On board AOM for down selection of repetition rate from single shot to 50MHz.

• Software controlled user variable pulse width from < 350fs to >10ps.

• All fiber single pass MOPA design from seed laser to main amplifier.

• On board pulse width monitor and energy monitor.

• Single box design with no distributed seed laser or pump diodes in a separate box.

• Seeder includes mode-locking SESAM shifter for long life operation.

• Three-point kinematic mount on laser head for superior long term stable operation in diverse ambient temperatures.

• Integrated onboard active cleaning engine to maintain cleanliness of laser housing in harsh environments throughout the life of the laser.

• HASS/HALT testing protocol, B10 > 22,500hrs

• RS232, Ethernet, and USB interface. Remote access possible from anywhere in world.

• Noise <0.25% rms in a 10Hz-10MHz band

6. Quantity: 1 Multi-wavelength Dual laser for multi-color imaging system and 1 Fixed-wavelength laser for optical stimulation

7. Delivery: Expected delivery date is 14 weeks after receipt of order. Delivery must be made to:

National Institute of Neurological Disorders and Stroke 35A Convent Dr.

Building 35A/ Rm 3D-734 Bethesda, MD 20892-00001Bethesda, MD 20892-00001.

No drop delivery allowed

8. Option(s): N/A