D_Purchase Description.pdf
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- Attached to
- Promethion flow cell packs for long-read sequencing Federal contract opportunity
- Solicitation number
- 75N95023Q00413-RFQ
About this file
This document describes a purchase description for procuring PromethION flow cell packs and related sequencing kits to support genomic research projects at the National Institute on Aging. The National Institute on Aging requires PromethION flow cell packs from Oxford Nanopore in quantities specified to enable high-output, long-read DNA and RNA sequencing of samples from various aging and epigenetics studies. Delivery of the products is to occur in two shipments within 90 days of award to buildings on the National Institute on Aging campus in Baltimore, Maryland. The related federal contract opportunity is a request for quotation under solicitation number 75N95023Q00413-RFQ issued by the Department of Health and Human Services National Institutes of Health National Institute on Drug Abuse to procure these PromethION flow cell packs and sequencing kits.
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Other files for this federal contract opportunity
| File | Type | Posted |
|---|---|---|
| D_Purchase Description-rev.pdf | ||
| D_FAR 52.212-5 Jun 2023.pdf | ||
| D_FAR 52.204-24 Nov 2021.pdf | ||
| D_FAR 52.225-6 Feb 2021.pdf | ||
| D_Invoicing Instructions With IPP 3-23.pdf | ||
| D_FAR 52.204-26 Oct 2020.pdf |
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Text version
FAR Part 11—Describing Agency Needs
PURCHASE DESCRIPTION
1. Statement of Need and Purpose: The National Institutes on Aging (NIA) requires the purchase of long-read sequencing PromethION flow cell packs.
2. Background Information and Objective:
The purpose of this acquisition is to procure full-length RNA and DNA sequencing flow cells, PCR-cDNA barcoding Kit, Ultra Long and Ligation DNA sequencing kits to support multiple researchers conducting studies in the Intramural Research Program, at the NIA. Nanopore flow cells will be used for collaborative projects and other IRP researchers. The projects include studying age-associated changes in gene expression and epigenetics (DNA methylation) in cell lines and in various animal tissues. RNA and DNA isolated from these samples will be sequenced using full-length RNA/cDNA-Seq to identify transcript isoforms, splice variants and using long-read DNA-seq to map global DNA methylation.
3. Generic Name of Product: PromethION Flow Cells
4. Purchase Description: PromethION Flow Cell packs for long-read DNA and RNA/cDNA
5. Salient characteristics/Project Requirements:
The Oxford Nanopore PromethION device is an electronic analysis system for use in scientific research. The core technology is built around a nanopore that can detect single molecule events including nucleic acids (DNA/RNA), proteins and small molecules. PromethION device is currently used by the NIA researchers.
The required flow cell packs are consumables (chemistry) used with PromethION devices that enable high-output, high-throughput sequencing of DNA or RNA samples. The flow cell kits are designed to allow pooling and running multiple libraries on Oxford Nanopore devices. It allows sequential runs of multiple sequencing libraries in the same flow cell.
6. Quantity:
Delivery 1 – Du/Kayla Promethion Flow Cells v 9.4.1 (Pack of 4) – Cat. No. FLO-PRO002 – QTY = 4 Sequencing Kit, Ultra Long v Kit 14 (6 rxns) – Cat. No. SQK-ULK114 – QTY = 6 Sequencing Kit, Ligation sequencing v Kit 14 (6 rxns) – Cat. No. SQK-LSK114 – QTY = 6 Sequencing Kit, PCR-cDNA Barcoding v Kit 111 (6 rxns) – Cat. No. SQK-PCB111.24 – QTY = 6 Flow Cell, MinION/GridION v10.4.1 – Cat. No. FLO-MIN114 – QTY = 1
Delivery 2 – De/Roberson Promethion Flow Cells v 9.4.1 (Pack of 4) – Cat. No. FLO-PRO002 – QTY = 4 Promethion Flow Cells v 10.4.1 (Pack of 4) – Cat. No. FLO-PRO114 – QTY = 4 Promethion Flow Cells v 9.4.1 (Pack of 3) – Cat. No. FLO-PRO002 – QTY = 1
FAR Part 11—Describing Agency Needs
7. Delivery: Delivery shall be made to the National Institute on Aging located at 251 Bayview BLVD, Baltimore, MD 21224 as shown below.
Delivery 1 – Du/Kaila Delivery 2 – De/Roberson
National Institute on Aging BRC building, Room 6C211A.
251 Bayview Blvd, Baltimore, MD 21224
National Institute on Aging BRC building, Suite 100 251 Bayview Blvd, Baltimore, MD 21224
The anticipated delivery date is within 90 days after award. Offerors may propose a different delivery time in accordance with standard commercial practice.
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