D_Purchase Description.pdf

PURCHASE DESCRIPTION

1. Statement of Need and Purpose:

The National Institute on Aging (NIA), Computational Biology & Genomics Core (CBGC) laboratory requires Chromium Next GEM Single Cell Gene Expression v3.1 Consumables to profile transcriptional changes in hundreds of thousands of cells.

2. Background Information and Objective:

The Chronium Next GEM v3.1 single cell gene expression with feature barcode technology consumables will allow the CBGC to capture between 10,000 to 80,000 single cells per experiment and mark them with a barcode and a second unique molecular code that will then be tagged to RNA. Following downstream procedures to first isolate and then sequence the RNA, these unique codes are read, deconvoluted and stratified to learn the nature of transcripts within each captured single cell. This procedure is highly valuable to distinguish the cellular complexity of a sample based on the nature of transcription profile, and along with turnkey analysis and software visualization tools, allows the CBGC to maximize insights from a wide range of sample types. These kits require a special set of indices to uniquely mark each sample, so data from multiple samples sequenced together can be separated based on sample identity.

There is a wide interest in the aging research field to understand how cellular heterogeneity contributes to different biological systems. This can be addressed by transcriptional profiling at single cell resolution across tens of thousands of cells, and single cell transcriptomics helps to distinguish cell types with different functions, and gain insights into cell subtypes and states that are specifically altered in aging, both in health and disease, This method will allow NIA to approach these goals with high efficiency. It is important to note that this system is the predominant single cell methodology in use on the market, and is currently successfully implemented in many ongoing studies within the NIA Intramural Research Program (IRP) research portfolio.

3. Generic Name of Product: Single cell gene expression kit

4. Purchase Description: Chromium Next GEM Single Cell 3' Kit v3.1 4 rxns and Dual Index Kit TT Set A 96 rxns

5. Salient characteristics:

All deliverables required under this contract shall be packaged, marked, and shipped in accordance with Government specifications. At a minimum, all deliverables shall be marked with the contract number and Contractor name. The Contractor shall guarantee that all required materials shall be delivered in immediate usable and acceptable condition.

Vendor must be able to provide these specifications:

• Be a smaller kit format (4 rxns, not 16 rxns) to avoid waste and experiement cross-contamination

• Enable single cell transcriptional profiling of up to millions of single cells.

• Allow single cell suspensions to be loaded onto the10S Genomics Chromium

Controller to be partitioned into GEMs for tagging of transcripts cell specific barcodes.

• Allow barcoded cDNA to be pooled for downstream processing and library preparation.

• Allow targeting of gene expression and feature profiling (e.g. cell surface proteins, guide RNA), to enable enhanced phenotypic profiling in thousands of individual cells using pre-designed or customized gene panels, as well as simultaneous assessment.

• Contain reagents to construct a library using the Feature Barcoding technology.

• Have precise and efficient microfluidics to allow a wide dynamic range of cells to be recovered in droplets in each <18 minute run with a low doublet rate, facilitating the profiling of precious and rare cell populations.

• Be compatible with low throughput, standard and high throughput workflows.

• After sequencing, downstream bioinformatics tools should be able to use cellular barcodes to group transcripts that originated from the same cell, to reveal transcriptome and/or the feature information of each individual cell.

6. Quantity:

Chromium Next GEM Single Cell 3' Kit v3.1, 4 rxns – 8 Dual Index Kit TT Set - 1

7. Delivery Date: Thirty (30) days after receipt of purchase order

8. Period of Performance: N/A

9. Option(s): N/A