D. Attachment 1. Purchase Description.pdf

Version 11-30-2016-1 FAR Part 11—Describing Agency Needs

PURCHASE DESCRIPTION

1. Statement of Need and Purpose:

The Neurological Disorders and Stroke’s (NINDS) Viral Immunology Section requires 10X Genomics Chromium Single Cell Gene Sequencing and Library Construction Kits or equal products. The NINDS Viral Immunology Section will use the kits to conduct single cell RNA sequencing and immune profiling, as well as explore the T cell receptor, B cell receptor, and transcriptional changes associated with HIV disease status.

2. Background Information and Objective:

The NINDS is a part of the National Institutes of Health (NIH) and conducts research into the causes, treatment, and prevention of neurological disorders and stroke. The NINDS Viral Immunology Section needs specific supplies to prepare libraries for sequencing patient samples and for gene expression analysis related to human immunodeficiency virus (HIV) disease status.

3. Product Information

a. Brand Name of Product: Chromium Single Cell Gene Sequencing Kits and

Library Construction Kit

b. Generic Name of Product: Single cell gene sequencing kits; library construction kit; TCR and BCR amplification kits

c. Manufacturer: 10X Genomics, Inc.

d. Product Description:

Product Description Product

Code Quantity 10X Genomics Chromium Next GEM Single Cell 5' Kit v2, 48 rxns 1000286 10 10X Genomics Dual Index Kit TT Set A 96 rxns 1000215 4 10X Genomics Library Construction Kit, 16 rxns 1000190 15 10X Genomics Chromium Single Cell Human BCR Amplification Kit, 16 rxns 1000253 8 10X Genomics Chromium Next GEM Single Cell 5' Kit v2, 16 rxns 1000263 8 10X Genomics Chromium Single Cell Human TCR Amplification Kit, 16 rxns 1000252 8 Shipping and Handling N/A 1

4. Salient Characteristics:

The required kits must be brand name or equal to the products listed in the “Purchase Description” section (above). Equal products not manufactured by 10X Genomics, Inc. must meet or exceed all of the following specifications:

Version 11-30-2016-1 FAR Part 11—Describing Agency Needs

• Be compatible with 10X Genomics’ Chromium Controller.

• Enable the identification and characterization of rare cell types.

• Allow analysis of heterogeneity.

• Enable cellular phenotyping with single cell RNA-seq to identify novel targets, biomarkers, and cell types and states without the need for pre-selected targets.

• Enable evaluation of mRNA and cell surface protein expression profiles within the same cell.

• Allow high-throughput and high-resolution functional genetic screens in tens of thousands of cells simultaneously.

• Allow assessment of comprehensive gene expression phenotypes.

• Have a shelf life of 6 to 12 months.

• Allow the generation and recovery of up to 10,000 cells in each GEM well.

• Allow reverse transcription of mRNA to cDNA in a Polymerase chain reaction (PCR) machine.

• Single Cell 5' Kit v2 kits, 16 rxns must include: gel beads, Dynabeads™

MyOne™ SILAN, fragmentation enzyme, fragmentation buffer, ligation buffer, DNA ligase, adaptor oligos, and amplification mix, RT reagent, RT enzyme, reducing agent, poly-dT RT primer, cleanup buffer, amplification master mix, and cDNA primers

• Single Cell 5' Kits v2, 48 rxns must include: partitioning oil, recovery agent, Chromium Next GEM Chip K, required gaskets, and a gasket cover aligned with chip where microfluidic flow is generated.

• Single cell BCR amplification kit must include: human B cell mix 1, human B cell mix 2, and amplification mix

• Single cell TCR amplification kit must include: human T cell mix 1, human T cell mix 2, and amplification kit

• Dual Index Kit TT Set A, 96 rxns must be able to demultiplex multiple samples in the same run of a RNA-sequencing pipeline and allow the addition of i7 sample indexes to each read

5. Delivery:

The Contractor shall deliver all products specified under Product Description above to the National Institute of Neurological Disorders and Stroke, Viral Immunology Section, 10 Center Driver, Rom 5C103, Bethesda, MD 20892, on Monday through Friday (excluding Federal holidays), between the hours of 8:00 am and 5:00 pm Eastern Time. The products shall be delivered in ice and/or dry ice as needed to maintain proper temperatures. The Contractor shall guarantee that all products are delivered to the Viral Immunology Section in immediate usable and acceptable condition.

The Contractor shall delivery all products specified herein within thirty (30) days after receipt of the Government’s purchase order.