D-Attachment PURCHASE DESCRIPTION Spinnig Disk Upgrades.pdf

Version 11-30-2016-1 FAR Part 11—Describing Agency Needs

PURCHASE DESCRIPTION

1. Statement of Need and Purpose: The purpose of this acquisition is to upgrade the BioPipeline LIVE - Eclipse Ti2-E with Robotics and Incubation System being used in the iPSC Neurodegenerative Diseases Initiative (INDI) Project within the new Center for Alzheimer’s Disease and Related Dementias.

2. Background Information and Objective: imaging technology improves to capture The NIA is working to create a national resource of isogenic sets of Alzheimer’s Disease and Related Dementias mutated iPSC lines, complimented by foundational phenotypic data sets from differentiated disease-relevant cells derived from these lines. NIA will need to provide upgrades for Phase Contrast that includes objectives to optimize the robotics system of an existing spinning disk microscope. Unable to currently correct the lenses due to the robotics arm inhibiting access to the objectives, the new objectives can auto correct with no need to manually adjust the objectives for optimal focus. Additional upgrades also allow for spheroid images as well as adding the ability to allow for bright field imaging which has become a new standard for imaging data analysis. The hardware will integrate into the BioPipeline LIVE - Eclipse Ti2-E with Robotics and Incubation System with NIS-Elements Modules software.

The purpose is for INDI research to be able to do fluorescent with an expanded set of lasers live cell imaging related to the pathogenesis of neurodegenerative diseases. The iPS cell lines are essential in studying the pathogenesis of neurodegenerative these diseases.

3. Generic Name of Product: Spinning disk microscope upgrades

4. Purchase Description:

The Upgrades include:

Nikon PFS+ACC 60X WI

LIDA Transmitted light source

External Phase

Silicone Objectives

Nikon Elements Offline modules

5. Salient characteristics

Vendor must be able to provide these specifications:

Nikon PFS+ACC 60X WI:

TI2-N-NDA-P PFS MOTOR NOSEPIECE ACC Product# MEP59396 QTY=1

CFI SR PLAN APO IR AC 60XWI Product# MRD07654 QTY=1 LIDA Transmitted light source:

LIDA LIGHT ENGINE, RGB CHANLS Product# 77060081 QTY=1 4 CHANL BREAK OUT CABLE F/LIDA Product# 77060082 QTY=1 NIS-ELEMENTS: TRIGGERED DEVICE CONTROL Product# MQS42780 QTY=1 to be added to key 026B01BF

Version 11-30-2016-1 FAR Part 11—Describing Agency Needs

External Phase:

TI2-T-BP-E E-TUBE BASE UNIT EXTERNAL PH Product# MEB55845 QTY=1 TI2-T-SULXPH STAGE UP LENS EXTERNAL PH Product# MEV54005 QTY=1 TI2-T-XPH-PH3 60X PH3 RING Product# MEH51700 QTY=1 TC-C-ML-PH3 LWD MODULE Product# MEH51300 QTY=1 Silicone Objectives:

CFI PLAN APO LAMBDA S 25XC SILICONE Product# MRD73250 QTY=1 CFI SR HP PLAN APO LAMBDA S 100X C SIL Product# MRD73950 QTY=1 30CC SILICONE IMMERSION OIL Product# MXA22179 QTY=1 Nikon Elements Offline modules NIS-ELEMENTS: AR PASSIVE SUA 1 YR Product# MQS31510 QTY=1

Passive Key #6CF955AB NIS-ELEMENTS MODULE: NIS.AI MODULE Product# MQS43200 QTY=1

NIS-ELEMENTS: LIM 2D/3D DECON TOOLS Product# MQS42700 QTY=1

6. Quantity: See Salient Characteristics for quantity.

7. Delivery Date: 60 days after receipt of completed purchase order.

8. Period of Performance: Not applicable

9. Option(s): Not applicable