D Attachment 1 Purchase Description.pdf

PURCHASE DESCRIPTION

1. Statement of Need and Purpose:

Acquisition of one PHERAstar FSX multimodal microplate reader (Brand name or equal)

2. Background Information and Objective:

The Tox21 and Robotics Screening Lab at NCATS seeks to purchase a PHERAstar FSX multimodal plate reader for integration on the fully automated Tox21 robotic screening platform (Brand name or equal). The robotic system currently features key elements for an automated screen (reagent dispenser, incubators, pintool, and robotic arms), and this PHERAstar FSX with assay stabilization multimode reader will be integrated onto the Tox21 robot to replace the dependence on outdated technology using the very old Envision reader. The addition of this PHERAstar plate reader will make it possible for assays to be run start to finish through data acquisition for a wide variety of assay types in a fully automated fashion. This has a high priority because we need this in time for a robotic upgrade.

3. Generic Name of Product Multimode Microplate Reader

4. Type of Purchase Description X Brand-name or Equal

5. Physical, Functional, and Performance Specifications

The PHERAstar FSX multimodal plate readers are commercially available items that are customized with options to fit the specific laboratory needs.

Part Number Line Item Description 0472-101A PHERAstar FSX Basic (Brand name or equal) 1100-472-401 Absorbance FS Spectrometer (Brand name or equal) 1100-472-501 High-End TRF/HTRF (Brand name or equal) 1100-472-502 TR-FRET Laser (Brand name or equal) Assay Stability System Advanced Assay Stability needed (Brand name or equal) Manufacturer's Warranty 1300-501 Control and Evaluation Software(Brand name or equal) Installation and Training 1400-G4M-TRF-337-665-620 Optic Module (Brand name or equal) 1400-G2M-FI-350-450 Optic Module (Brand name or equal) 1400-G3M-FI-485-590-520 Optic Module (Brand name or equal) 1400-G3M-FI-410-460-530-460 Optic Module (Brand name or equal)

The brand name or equal multimodal plate reader must meet requirements and have the capabilities detailed below:

1. Eleven measurement principles: (1) Fluorescence intensity, (2) UV/Vis Absorbance, (3) Luminescence, (4) Time-Resolved Fluorescence, (5) Simultaneous Dual Emission, (6) Fluorescence Resonance Energy Transfer(FRET), (7) Laser-based AlphaScreen® / AlphaLISA®/AlphaPlex®, (8) Fluorescence Polarization, (9) HTRF® ,(10) Time-Resolved fluorescence resonance energy transfer (TR-FRET), (11) Bioluminescence resonance energy transfer (BRET)

2. Simultaneous dual emission in all measurement modes, both emission wavelengths are collected at the same time. Greatly enhances speed, throughput, and accuracy – especially for FRET(TR-FRET)/BRET/FP assays

3. Four PMTs that are optimized and matched for various measurement modes – no other reader on the market has this capability. PMTs are automatically used when needed with no user intervention

4. Ultra-Fast UV/Vis spectrometer that allows for full-spectrum absorbance measurement to be taken in less than 1 second per well at 1,2,5 and 10nm resolution

5. Direct Optic Bottom Reading- unmatched bottom reading for cell-based assays is achieved through innovative engineering using a series of software controlled lenses and mirrors while allowing for automatic software controlled switching between top and bottom reading

6. Optional on-board integrated reagent injectors for assays that acquire the ability to monitor a signal before, during, and after the addition of a reagent to a well. Inject and read simultaneously

7. Extremely fast read times- 14s for a 384 well plate, and 27s for a 1536 well plate (1 flash)

8. Advanced assay stability system which enables the microplate reader to match room temperature conditions and to be maintained at any temperature between 18 and 45°C.

9. Z height focus most accurate in the industry – 100 micron resolution for fantastic sensitivity

10. Assay modules with ALL filters and dichroics contained in one component – no swapping of filters, wheels, dichroics, mirrors, or other pieces necessary

11. Three excitation sources: Standard high intensity xenon flashlamp for excitation and direct light path illumination of wells; optional AlphaScreen solid state red laser for enhanced performance;

and optional pulsed nitrogen UV laser at 337nm for TRF and TR-FRET such as HTRF (lanthanide based), LanthaScreen and DELFIA

12. Reads 6-3456 well plates - user definable plate formats

13. Easily interfaced with robotic systems through DDE or ActiveX interface

14. Data analysis Software included at no cost, can be used on as many machines as you like, no licensing arrangements, only available for PCs

15. FDA 21 CFR Part 11 Compliance included at no additional charge

16. Maximum footprint 45cm W X 51cm D x 47cm H

17. 50 kg or less in weight

18. Optional demo unit for use with system integration

6. Quantity

One

7. Delivery Date or Period of Performance 6-7 weeks ARO

8. Delivery Location

Address: National Center for the Advancement of Translational Sciences, 9800 Medical Center Dr, Bldg C, Rockville, MD 20850 Attention: Carleen Klumpp-Thomas