css2_75N92021Q0135.pdf

75N92021Q0135

COMBINED SYNOPSIS / SOLICITATION

WORKFORM

(1) Action Code: Combined Synopsis/Solicitation

(2) Date: June 23

(3) Year: 2020

(4) Contracting Office Zip Code: 20892

(5) Classification Code: 6640

(6) Contracting Office Address:

6701 Rockledge Drive Rockledge II Bethesda, MD 20892

(7) Subject/Title: Acquisition of Chromium Single Cell Library, Chromium Single Cell ATAC Library and Gel Bead kits

(8) Proposed Solicitation Number: 75N92021Q0135

(9) Closing Response Date: June 28, 2021

(10) Contact Point(s): Tahir Seeba, Contract Specialist

(11) Contract Award and Solicitation Number: TBD

(12) Contract Award Dollar Amount: TBD

(13) Contract Line Item Number(s): TBD

(14) Contractor Award Date: TBD

(15) Contractor Name: TBD

(16) Description:

(i) This is a combined synopsis/solicitation for commercial items prepared in accordance with the format in Subpart 12.6 as supplemented with additional information included in this notice. This announcement constitutes the only solicitation; quotes are being requested and a written solicitation will not be issued.

(ii) The solicitation number 75N92021Q0135 and the solicitation is issued as a request for quotation (RFQ).

(iii) The solicitation document, the incorporated provisions, and clauses are those in effect through Federal Acquisition Circular FAC 2021-05 effective March 10, 2021.

(iv) This requirement has no business size standard restriction. The associated NAICS code 334516 and the small business size standard is 1,000 employees.

This acquisition is being conducted in accordance with the procedures of FAR Part 12 and FAR Part 13.

(v) It is anticipated that the award from this combine synopsis/solicitation will be a Firm Fixed Price Purchase Order. The table below lists the Item Number(s), quantities and units of measure.

Item Product Descriptions Unit Quantity 1000262 3' Feature Barcode Kit, 16 rxns ea 2 1000212 Single Index Kit N Set A, 96 rxn ea 2 1000268 Chromium Next GEM Single Cell 3' Kit v3.1, 16 rxns ea 2

1000242 Dual Index Kit NT Set A, 96 rxn ea 1 1000215 Dual Index Kit TT Set A 96 rxns ea 2 1000190 Library Construction Kit, 16 rxns ea 2 1000256 5' Feature Barcode Kit, 16 rxns ea 2 1000286 Chromium Next GEM Chip K Single Cell Kit, 48 rxns ea 2

1000263 Chromium Next GEM Single Cell 5' Kit v2, 16 rxns ea 2 1000253 Chromium Single Cell Human BCR Amplification

Kit, 16 rxns ea 1

1000252 Chromium Single Cell Human TCR Amplification Kit, 16 rxns ea 1

1000250 Dual Index Kit TN Set A, 96 rxn ea 2 1000230 Chromium Next GEM Chip J Single Cell Kit, 16 rxns ea 1

1000283 Chromium Next GEM Single Cell Multiome ATAC + Gene Expression Reagent Bundle, 16 rxns ea 1

1000120 Chromium Next GEM Chip G Single Cell Kit, 48 rxns ea 1

(vi) Statement of Need:

Background:

The NIAMS Genomic Technology Section is charged with providing genome-scale, high throughput sequencing services for researchers within the institute.

The Core Facility, like most other Institutes at NIH and sequencing centers around the world, adopts new emerging technologies in order to maintain the leading edge on genomics and epigenetic research areas.

Currently, NIAMS core facility uses the 10X Genomics Chromium Controller Single Cell Platform that is a molecular barcoding and analysis suite that delivers cell-by-cell 3’ counting of mRNA transcripts for many tens of thousands of cells per run. The platform supports a broad range of applications, including single-cell spatial transcriptomics, cancer-cell transcriptomics and cell-type identification and discovery. Because the platform works with short read sequencers it is configured to be compatible with NIAMS Illumina sequencing systems and will be a very important tool for our researchers to continue performing genomic and epigenetic research projects.

The Chromium Controller Single-Cell Platform provides high throughput reagent delivery using a microfluidic chip. The 8-sample cartridge is loaded with the gel beads, a 3’ counting reagent, a cell suspension, and an oil-surfactant solution.

Reagents and cells are combined at a microfluidic junction where gel bead-containing emulsions (GEMs) are generated. GEMs are created in such a way that single cells are partitioned and >90% of reactions contain a unique cell-linked molecular barcode. The simple workflow creates up to 100,000 GEMs per channel in a 10-20 minutes instrument run time.

To support the research of NIAMS Investigators, there is need to purchase reagents compatible with this system. The Chromium Controller Single Cell Platform uses the Chromium Single Cell Library & Gel Bead Kit, the Chromium Single Cell ATAC Library & Gel Bead Kit, Library Construction, Single Cell and Index kits to produce epigenetic information of thousands of cells of up to eight different cell populations. These tools will support several research projects carried out to better understand the genetic determinants of autoimmune, auto inflammatory and musculoskeletal diseases and gain insights into the pathogenesis of genetic and immunologic diseases at a single-cell level.

The work of the NIAMS Genome Analysis Core Facility depends heavily on sequencing of DNA Libraries from tissues, cell lines and single cell populations from various species. The purchase of these reagents is needed to operate and conduct experiments in the Chromium Controller Platform; it is fundamental to access the cutting-edge technology in the area of single-cell epigenetic research that enable discovery of gene expression dynamics and molecular profiling of individual cell types. This acquisition fulfills the mission of the Core Facility on meeting the demand for new tools to support NIAMS research.

Objective:

The purpose of this requirement is the to acquire reagents compatible with NIAMS Chromium Controller Single-Cell Platform.

Scope:

The Contractor shall provide reagents brand name or equal specifications to the reagents listed in the above table in section 16(v).

Delivery Date:

The offeror shall include the delivery lead time in their quote.

Place of Performance:

Genomic Technology Section 9000 Rockville Pike , Building 50, Room 1345 (Office), 1342 (Lab.)

Bethesda, MD 20892

The required delivery is FOB Destination, a duly authorized representative will perform inspection and acceptance of the reagents to be provided.

The Contractor will have reagents delivered no later than August 13, 2021, as a Period of Performance once an award is established to complete the work.

Coordination between the Vendor and the Site POC will be required after the contract award to establish a start date in order to de-conflict any known mission events or complete any other required site preparations.

Point of contact:

Gustavo Guitierrez-Cruz

Genomic Technology Section 9000 Rockville Pike , Building 50, Room 1345 (Office), 1342 (Lab.)

Bethesda, MD 20892

(vii) The provision at FAR clause 52.211-6, Brand Name or Equal, applies to this acquisition

(viii) The provision at FAR clause 52.212-1, Instructions to Offerors – Commercial

Items, applies to this acquisition.

- The Government request discounts

- The required item must meet the brand name or equal specifications

- Quote shall include warranty,

(ix) The provision at FAR clause 52.212-2, Evaluation – Commercial Items is applicable to this acquisition.

The award will be made to the Contractor providing the most advantageous quote to the Government. Technical and cost factors will be considered. To be considered technically acceptable, the offeror must provide an equipment brand name or equal to reagents in listed in section 16v.

(x) FAR clause 52.212-3, Offeror Representations and Certifications – Commercial

Items, is applicable. An offeror shall complete only paragraphs (b) of this provision if the offeror has completed the annual representations and certificates electronically via http://www.acquisition.gov.

If an offeror has not completed the annual representations and certifications electronically at the System for Award Management (SAM) website, the offeror shall complete only paragraphs (c) through (p) of this provision.

(xi) FAR clause at 52.212-4, Contract Terms and Conditions – Commercial Items, applies to this acquisition.

(xii) FAR clause at 52.212-5, Contract Terms and Conditions Required to Implement Statutes or Executive Orders – Commercial Items, applies to this acquisition.

Including the following clauses and Prohibitions:

I. 52.219-28, Post Award Small Business Program Representation II. 52.222-3, Convict Labor

III. 52.222-21, Prohibition of Segregated Facilities IV. 52.222-26, Equal Opportunity V. 52.222-36, Equal Opportunity for Workers with Disabilities VI. 52.222-50, Combating Trafficking in Persons

VII. 52.225-13, Restrictions on Certain Foreign Purchases VIII. 52.223-18, Encouraging Contractor Policies to Ban Text

Messaging while Driving.

IX. 52.225-13, Restrictions on Certain Foreign Purchase X. 52.232-39, Unenforceability of Unauthorized Obligations XI. 52.204-23, Prohibition on Contracting for Hardware, Software, and

Services Developed or Provided by Kaspersky Lab and Other Covered Entities

XII. 52.204-24, Representation Regarding Certain Telecommunications and Video Surveillance Services or Equipment.

XIII. 52.204-25, Prohibition on Contracting for Certain Telecommunications and Video Surveillance Services or Equipment.

XIV. FAR 52.227-14, Rights in Data - Alternate IV (December 2007) XV. 52.232-33, Payment by Electronic Funds Transfer—System for

Award Management (JUL 2013) (31 U.S.C. 3332) XVI. 52.204-23, Prohibition on Contracting for Hardware, Software, and

Services Developed or Provided by Kaspersky Lab and Other Covered Entities.

XVII. FAR 52.232-39 – Unenforceability of Unauthorized Obligations

(xiii) Additional Contract Requirements: None

(xiv) The Defense Priorities and Allocations System (DPAS) and assigned rating are not applicable.

(xv) Offers shall be submitted via the FedConnect web portal (www.fedconnect.net).

The due date for receipt of offers is 12:00 noon Eastern Time on June 28, 2021.

Vendors can register with FedConnect at https://www.fedconnect.net/FedConnect/default.htm. Please note that FedConnect is used by multiple federal agencies and therefore FedConnect assistance will be provided by Compusearch Software Systems, not the NHLBI OA. More information about registration requirements can be found by downloading the FedConnect Ready, Set, Go! Guide at https://www.fedconnect.net/fedconnect/Marketing/Documents/FedConnect_Read y_Set_Go.pdf.

For assistance in registering or for other FedConnect technical questions please call the FedConnect Help Desk at (800) 899-6665 or email at support@fedconnect.net.

(xvi) Contact Points:

Tahir Seeba, Contract Specialist http://www.fedconnect.net/ Solicitation 75N92021Q0135

(17) Place of Contract Performance:

NIH/NIAMS

10 Center Drive Building 10 Room 10N318, Bethesda, MD 20892.

Coordination between the Vendor and the Site POC will be required after contract award, to establish start date in order to de-conflict any known mission events or complete any other required site preparations. The Contractor is required to clean-up after installing the equipment and, maintain a safe working area at all times.

(18) Set-aside Status: Total Small Business http://www.fedconnect.net/ https://www.fedconnect.net/FedConnect/default.htm https://www.fedconnect.net/fedconnect/Marketing/Documents/FedConnect_Ready_Set_Go.pdf https://www.fedconnect.net/fedconnect/Marketing/Documents/FedConnect_Ready_Set_Go.pdf mailto:support@fedconnect.net http://www.fedconnect.net/