Combined Synopsis Solicitation.pdf

75N92021Q0136

COMBINED SYNOPSIS / SOLICITATION

WORKFORM

(1) Action Code: Combined Synopsis/Solicitation

(2) Date: June 23

(3) Year: 2021

(4) Contracting Office Zip Code: 20892

(5) Classification Code: 6640

(6) Contracting Office Address:

6701 Rockledge Drive Rockledge II Bethesda, MD 20892

(7) Subject/Title: Brand Name or Equal Cytek Aurora-CS 5L Full Spectrum Sorter and Aurora 5-Laser UV/V/B/YG/R Systems.

(8) Proposed Solicitation Number: 75N92021Q0136

(9) Closing Response Date: July 07, 2021

(10) Contact Point(s): Tahir Seeba, Contract Specialist

(11) Contract Award and Solicitation Number: TBD

(12) Contract Award Dollar Amount: TBD

(13) Contract Line Item Number(s): TBD

(14) Contractor Award Date: TBD

(15) Contractor Name: TBD

(16) Description:

(i) This is a combined synopsis/solicitation for commercial items prepared in accordance with the format in Subpart 12.6 as supplemented with additional information included in this notice. This announcement constitutes the only solicitation; quotes are being requested and a written solicitation will not be issued.

(ii) The solicitation number 75N92021Q0136 and the solicitation is issued as a request for quotation (RFQ).

(iii) The solicitation document, the incorporated provisions, and clauses are those in effect through Federal Acquisition Circular FAC 2021-05 effective March 10, 2021.

(iv) This requirement has no business size standard restriction. The associated

NAICS code 423490 and the small business size standard is 150 employees.

This acquisition is being conducted in accordance with the procedures of FAR Part 12 and FAR Part 13.

(v) It is anticipated that the award from this combine synopsis/solicitation will be a Firm Fixed Price Purchase Order. The table below lists the Item Number(s), quantities and units of measure, inclusive of any applicable options.

Item Product Descriptions Unit Quantity 1 Cytek Aurora Cell Sorter

Cytek Aurora-CS 5L System Cytek® Aurora CS Full Spectrum Sorter Cytek Aurora CS 5 Laser UV/V/B/YG/R (64 + Channel) Includes:

5-laser full spectrum sorter with with tube and plate sorting, sample and collection tube cooling and heating, built-in primary aerosol containment, various nozzle sizes.

Workstation (computer, monitor, keyboard, mouse) • 1 additional copy of SpectroFlo software • QC and consumable parts kit • 1-year on-site warranty Seamless compatibility with Cytek® Aurora analyzer 3-day on-site training: Three-day training:

lectures on the fundamentals of spectral cytometry, panel design and sorting w/ hands-on operator training (max. 4 students for operator

EA 1

2 Baker Biosafety Cabinet - US - CY401 120VAC 20A

EA 1

3 Cytek™ Aurora Cytek Aurora 5 Laser UV/V/B/YG/R (64 + 3 Channel) Includes: • 5-laser flow cytometer • Workstation

EA 1

(computer, monitor, keyboard, mouse) • 1 additional copy of SpectroFloTM • Fluidic reservoirs • QC and consumable parts kit • 1-year on-site warranty N7-00003 1.00 399,000.00 337,155.00 337,155.00

4 Cytek Auto-Loader system (ALS) 2.0 EA 1

(vi) Statement of Need:

Background:

The National Institutes of Health (NIH), a part of the U.S. Department of Health and Human Services, is the nation’s medical research agency — making important discoveries that improve health and save lives. The mission of the (NIAMS) is to support research into the causes, treatment, and prevention of arthritis and musculoskeletal and skin diseases; the training of basic and clinical scientists to carry out this research; and the dissemination of information on research progress in these diseases. The NIAMS Flow Cytometry Facility (FCF) provides research support to members of NIAMS IRP and its collaborators.

Serving all NIAMS investigators, both research and clinical, the FCF operates four flow cytometry cell sorters as well as oversees the use of six analyzers including a 5-laser, 64+3 channel Cytek Aurora spectral cytometer. The FCF supports approximately 120+ users seven days a week. Cell sorting services are provided Monday through Friday. Each instrument supports multiple experiments each day, and each instrument is fully utilized.

Objective:

The purpose of this requirement is to provide NIAMS investigators with state-of-the-art immunological assays instruments to able to develop a higher parameter staining panels using the existing 40-color Cytek Aurora spectral cytometer (purchased in 2018), and to able to easily sort and discover populations using compatible optical detectors and cell sorters

Scope:

The contractor shall provide and install an instrument with features brand name or equal to the Cytek Aurora-CS 5L Full Spectrum Sorter and Cytek Aurora 5- Laser UV/V/B/YG/R Systems as listed in section 16(v), including system software and 12 months manufacturer’s full warranty including parts and labor.

Minimum Technical Requirements

• Be a stand-alone multi-parameter spectral analyzing cytometer and cell sorter system, and include all required acquisition and analysis software

• Able to simultaneously discriminate up to 40 highly overlapping fluorochromes using Coarse Wavelength Division Multiplexing (CWDM) detection arrays.

• Capable of full spectrum unmixing

• Include three (3) light scatter parameters

• Incorporate side scatter detection laser (violet);

• Capable of small particle resolution down to 0.1 microns

• Incorporate five (5) spatially separated lasers as follows: UV, 355nm at

20mWatt minimum; violet, 405 nm at 100mWatt minimum; blue; 488 nm at 50mWatt minimum; yellow green, 561nm at 50mWatt minimum; red, 640 nm at 80m Watt minimum

• Accommodate a variety of sample tube formats for manual sample delivery

• Cell sorter must:

o include sample and collection tube cooling and heating o be capable of multi-well plate sample delivery o be capable of variable sample handling and variable sort collection o be capable of handling 70um and 100um nozzle sizes o include primary and secondary biosafety controls to include (spell out) High-Efficiency Particulate Air (HEPA) filter (primary) for sort chamber aerosol management and Biosafety Class 2 Type A2 cabinet (secondary) tested to major WW biosafety standards o include 1 additional copy of acquisition/analysis software, QC and consumable parts kits

• Analyzer must:

o be capable of multi-well plate sample loading o include in-line flow meter capable of bead-less absolute cell counts o must include 1 additional copy of acquisition/analysis software, fluidic reservoirs, and QC and consumable parts kits

Computer Hardware/Software:

• Workstation for instrument control software (computer, 4K UHD monitor(s), keyboard, mouse)

Minimum Preventive Maintenance and Repair Service Requirements for the stand-alone multi-parameter spectral analyzing cytometer and cell sorter systems are:

• Two (2) planned on-site preventive maintenance visit for routine certification and calibration per contract year.

• Unlimited on-site corrective/remedial maintenance/repair visits which includes parts, labor, and travel.

• Response time within 24 hours after receipt of service call, and on-site corrective/remedial maintenance/repair visits executed within two (2) business days after receipt of service call.

• Access by the NIAMS Technical Representative (TR) and system operator personnel to the manufacturer’s call center for technical assistance, which is staffed by senior engineers to provide a high level of expertise for troubleshooting the instrument.

• Software, firmware, and application updates as required by the OEM.

• Priority access to technical support (e-mail and telephone) Mondays -

Fridays (excluding Federal Holidays) between the hours of 7:00 AM - 5:00 PM Eastern (Prevailing) Time.

• All maintenance activities shall be performed by formally trained and certified technicians and/or engineers, following Original Equipment Manufacturer (OEM) specifications, manuals, and service bulletins, using OEM replacement parts, components, subassemblies, etc.

• All preventive maintenance and repair service pricing shall be inclusive of labor, travel, replacement parts, components, subassemblies, etc.

Other Requirements:

• 3-day on-site training: Three-day training: lectures on the fundamentals of spectral cytometry, panel design and sorting w/hands-on operator training (max.

4 students for operator).

• Acceptance of BD Biosciences FACSCanto RUO and FACSRIA IIIu as trade-ins.

• Instruments must be compatible with existing NIAMS’ 40-color Cytek Aurora spectral cytometer, Cytek SpectroFlo and generate FCS (Flow Cytometry Standards) files consistent with the third-party FlowJo flow cytometry data analysis software.

Delivery Date:

The offeror shall include the delivery lead time in their quote.

Place of Performance:

Genomic Technology Section 9000 Rockville Pike , Building 50, Room 1345 (Office), 1342 (Lab.)

Bethesda, MD 20892

The required delivery is FOB Destination, a duly authorized representative will perform inspection and acceptance of the reagents to be provided.

The Contractor will have reagents delivered no later than August 13, 2022, as a Period of Performance once an award is established to complete the work.

Coordination between the Vendor and the Site POC will be required after the contract award to establish a start date in order to de-conflict any known mission events or complete any other required site preparations.

Point of contact:

James Simone National Institutes of Health, NIAMS 10 Center Drive Building 10, Room 10C208 Bethesda, MD 20892

(vii) The provision at FAR clause 52.211-6, Brand Name or Equal, applies to this acquisition

(viii) The provision at FAR clause 52.212-1, Instructions to Offerors – Commercial

Items, applies to this acquisition.

(ix) The provision at FAR clause 52.212-2, Evaluation – Commercial Items is applicable to this acquisition.

The award will be made to the Contractor providing the most advantageous quote to the Government. Technical and cost factors will be considered. To be considered technically acceptable, the offeror must provide an instrument brand name or equal to the instruments listed in section 16v. and meet the requirements of section 16vi..

(x) FAR clause 52.212-3, Offeror Representations and Certifications – Commercial

Items, is applicable. An offeror shall complete only paragraphs (b) of this provision if the offeror has completed the annual representations and certificates electronically via http://www.acquisition.gov.

If an offeror has not completed the annual representations and certifications electronically at the System for Award Management (SAM) website, the offeror shall complete only paragraphs (c) through (p) of this provision.

(xi) FAR clause at 52.212-4, Contract Terms and Conditions – Commercial Items, applies to this acquisition.

(xii) FAR clause at 52.212-5, Contract Terms and Conditions Required to Implement Statutes or Executive Orders – Commercial Items, applies to this acquisition.

Including the following clauses and Prohibitions:

I. 52.219-28, Post Award Small Business Program Representation II. 52.222-3, Convict Labor

III. 52.222-21, Prohibition of Segregated Facilities IV. 52.222-26, Equal Opportunity V. 52.222-36, Equal Opportunity for Workers with Disabilities VI. 52.222-50, Combating Trafficking in Persons

VII. 52.225-13, Restrictions on Certain Foreign Purchases VIII. 52.223-18, Encouraging Contractor Policies to Ban Text

Messaging while Driving.

IX. 52.225-13, Restrictions on Certain Foreign Purchase X. 52.232-39, Unenforceability of Unauthorized Obligations XI. 52.204-23, Prohibition on Contracting for Hardware, Software, and

Services Developed or Provided by Kaspersky Lab and Other Covered Entities

XII. 52.204-24, Representation Regarding Certain Telecommunications and Video Surveillance Services or Equipment.

XIII. 52.204-25, Prohibition on Contracting for Certain Telecommunications and Video Surveillance Services or Equipment.

XIV. FAR 52.227-14, Rights in Data - Alternate IV (December 2007) XV. 52.232-33, Payment by Electronic Funds Transfer—System for

Award Management (JUL 2013) (31 U.S.C. 3332) XVI. 52.204-23, Prohibition on Contracting for Hardware, Software, and

Services Developed or Provided by Kaspersky Lab and Other Covered Entities.

XVII. FAR 52.232-39 – Unenforceability of Unauthorized Obligations.

(xiii) Additional Contract Requirements: None

(xiv) The Defense Priorities and Allocations System (DPAS) and assigned rating are not applicable.

(xv) Offers shall be submitted via the FedConnect web portal (www.fedconnect.net).

The due date for receipt of offers is 12:00 noon Eastern Time on July 07, 2021.

Vendors can register with FedConnect at https://www.fedconnect.net/FedConnect/default.htm. Please note that FedConnect is used by multiple federal agencies and therefore FedConnect assistance will be provided by Compusearch Software Systems, not the NHLBI OA. More information about registration requirements can be found by downloading the FedConnect Ready, Set, Go! Guide at https://www.fedconnect.net/fedconnect/Marketing/Documents/FedConnect_Read y_Set_Go.pdf.

http://www.fedconnect.net/ https://www.fedconnect.net/FedConnect/default.htm https://www.fedconnect.net/fedconnect/Marketing/Documents/FedConnect_Ready_Set_Go.pdf https://www.fedconnect.net/fedconnect/Marketing/Documents/FedConnect_Ready_Set_Go.pdf

For assistance in registering or for other FedConnect technical questions please call the FedConnect Help Desk at (800) 899-6665 or email at support@fedconnect.net.

(xvi) Contact Points:

Tahir Seeba, Contract Specialist http://www.fedconnect.net/ Solicitation 75N92021Q0136

(17) Place of Contract Performance:

NIH/NIAMS

10 Center Drive Building 10 Room 10N318, Bethesda, MD 20892.

Coordination between the Vendor and the Site POC will be required after contract award, to establish start date in order to de-conflict any known mission events or complete any other required site preparations. The Contractor is required to clean-up after installing the equipment and, maintain a safe working area at all times.

(18) Set-aside Status: Total Small Business mailto:support@fedconnect.net http://www.fedconnect.net/