C04_SolicitationAttachment1SOW_1232SA26Q0915.pdf
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- Attached to
- Phage Library Screening Federal contract opportunity
- Solicitation number
- 1232SA26Q0915
About this file
This is a Statement of Work (SOW) for a phage library screening service contract to identify peptide sequences that bind to bovine and/or porcine receptors. The Foreign Animal Disease Research Unit at USDA-ARS at the National Bio and Agro-Defense Facility (NBAF) seeks this service to discover peptides for vaccine development against foreign animal diseases.
The contractor must perform the following tasks with specified performance periods: quality control (QC) of provided proteins within 12 days of receipt; purchase two different phage display libraries per protein; immobilize target proteins within 14 days of QC completion; conduct three rounds of negative and positive selection with phage amplification within 28 days of target immobilization; select and test at least 30 blue-plaque clones via ELISA and sequencing within 35 days of library screening; and deliver 6-12 candidate peptide sequences with corresponding amino acid sequences, nucleotide sequences, and raw sequencing data. Additionally, the contractor must provide a minimum of 50,000 NGS reads per protein per library within 49 days of order receipt and final payment. The contractor must renounce all intellectual property rights to discovered sequences and maintain confidentiality regarding the service. The government will furnish up to three purified His-tagged proteins expressed in cultured mammalian cells, retain ownership of all discovered sequences, and provide payment at 50 percent upon service order with the remainder due net 30.
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| File | Type | Posted |
|---|---|---|
| C04_SolicitationAttachment2WageDetermination_1232SA26Q0915.pdf | ||
| C04_Solicitation_1232SA26Q0915.pdf |
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Attachment 001 Statement of Work (SOW) Project: Phage library screening to identify peptide sequences binding bovine and porcine receptors
GENERAL INFORMATION
1. Scope of Work: This Statement of Work is to procure phage library screening service contract to identify peptide sequences binding bovine and/or porcine receptors
Service Contract:
The service plan includes the following:
1. Phage display libraries and target protein QC including peptide library expansion if needed.
2. Negative and positive phage library selection.
3. Sequencing and affinity testing of selected blue-plaque clones.
4. Deliverables: 6–12 candidate peptide sequences, together with the corresponding amino acid sequences, nucleotide sequences, and raw sequencing data.
2. Background: Foreign Animal Disease Research Unit, USDA-ARS at the National Bio and
Agro-Defense Facility (NBAF) develops countermeasures such as vaccines for the prevention and control of foreign animal diseases (FAD). Currently, there are several drawbacks of available FAD vaccines. Phage peptide display library screening is a powerful technology that can be used to identify peptides that can bind to specific receptors involved in the immune response. The discoveries of the peptide sequences can be implemented in the vaccine designs to improve the vaccine efficacy.
CONTRACTOR REQUIREMENTS
3. Technical Requirements/Tasks:
3.1 – To QC the proteins provided by the USDA scientist.
Period of performance: 12 days after receiving proteins.
3.2 – To purchase two different phage display libraries for the screening of each protein provided by USDA-ARS scientist.
3.3 – Target immobilization of provided proteins.
Period of performance: 14 days after QC.
3.4 – Perform 3 rounds of negative and positive selection, followed by amplification of the recovered phage Period of performance: 28 days after target immobilization.
3.5 – Select at least 30 blue-plaque clones for and affinity testing with ELISA and sequencing.
Period of performance: 35 days after library screening.
3.6 – Deliverables: 6–12 candidate peptide sequences, together with the corresponding amino acid sequences, nucleotide sequences, and raw sequencing data
3.7 – To provide a minimum of 50,000 NGS reads per protein per library solely to the USDA scientist.
Period of performance: 49 days after receipt of order and after receipt of the remaining final payment.
3.8 – To renounce the IP of all sequences discovered in the project and not to disclose the information on this service to third parties.
4. Government Furnished:
4.1 – To provide up to three purified His-tagged proteins that are expressed in cultured mammalian cells with the quantities requested by the contractor.
4.2 – The USDA scientist owns IP of all sequences discovered in the project
4.3 – To provide 50% of the payment upon the order of the service and remainder net 30.
| GENERAL INFORMATION |
| CONTRACTOR REQUIREMENTS |
| 3. Technical Requirements/Tasks: |
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