Brand Name Justification - Olympus upgrade.pdf

Brand Name Justification – Olympus upgrade to FV3000 1

Brand Name Justification

Project title: Upgrade existing Olympus FV1000 to the Olympus FV3000

Purpose and Objectives:

Through market research, the National Center for Complementary and Integrative Health (NCCIH) has established this acquisition as a brand name requirement. They have identified the need to purchase an Olympus FV3000 from Olympus (through Alliant Enterprises, LLC) to further their scientific research in understanding neural circuits in rodents. NCCIH requires Olympus products to upgrade their existing system since this is a previous generation microscope being upgraded to include new features and functionalities and is more cost efficient than buying a new system. These upgrades are required to allow scientist to advance their research by leveraging recent advances in florescent detection of proteins and RNAs in whole cleared tissue to image many different types of molecules in the same cells in intact brains simultaneously. For their ongoing studies, NCCIH needs to upgrade their Olympus confocal imaging system which can currently acquire two channels at a time, has slow acquisition speeds, outdated components/software and are no longer supported by the company. The custom upgrades to their existing microscope and their newer system will allow NCCIH to adapt their existing confocal microscope to perform four channel spectral imaging with greater speed and sensitivity needed for their ongoing experiments. This update to their current Olympus FY1000 will allow NCCIH to conduct cutting edge research in molecules, cells and neural circuits involved in touch and pain sensation research.

Specifically, NCCIH will be upgrading their existing Olympus FV1000 Confocal Microscope to an Olympus FV3000 confocal system. They need to acquire and separate additional wavelengths, image in 3D (volumes) with high speed and sensitivity. Therefore, NCCIH needs to add new filters, lasers, scan heads, detectors, objective stage and software to their current system. Adding these specific parts to enable multichannel volumetric imaging will greatly improve the ability of their existing system to further their scientific research in understanding neural circuits in rodents.

Due to these dependencies, the Olympus FV3000 is currently the only system that achieves these characteristics.

Proposed manufacturers/Original Equipment Manufacturer (OEM)s:

Olympus Corporation of the Americas, 48 Woerd Avenue, Waltham, MA 02453-3824

Specifications:

Olympus FV3000 confocal system

The vendor and its business partners shall deliver this system to NCCIH. The system requirements are, at a minimum:

• BX63LF;BX63 UPRIGHT MICROSCOPE FRAME FOR LASER CONFOCAL USE

• FV31-RFAAD; Nosepiece arm plus riser for BX63L electrophysiology configuration

• FV31-RFABXL; Single Deck fluorescence illumination unit

• U-MCZ-1-2; FOCUS CONTROL MODULE FOR BX63/IX83

• Interface Cable for IX3/BX3/STM7 - 200CM

• BX63L-CBH;CONTROL BOX FOR BX63LF

• DP21-DKT; General IEEE1394a cable (9pin to 6pin) for BX3-CBH, IX3-CBH, or DP21 with Desktop PC.

2.7m

• Eyepiece for BX/IX, 10x w/ =24mm t=1.5mm Retical Shelf, FN22

• Eyepiece for BX/IX, 10x Focusing w/ =24mm t=1.5mm Retical Shelf, FN22

• UYCP-11;US STYLE 3-PRONG POWER CORD

• FV30-ETTR;LASER SAFE ERECT IMAGE TILTING TRINOCULAR

• U PL XAPO 10X OBJECTIVE, OFN26.5, NA0.40, WD3.1mm

• U PL XAPO 20X OBJECTIVE, OFN26.5, NA0.80, WD0.6mm

• UPLSAPO30XS; U Plan S-Apo N 30X Si Oil Objective, NA 1.05, WD 0.80MM, W/CC

Brand Name Justification – Olympus upgrade to FV3000 2

• BX63L-DSGNPS;CODED SWING NOSEPIECE

• FV30-TD;TRANSMITTED DETECTOR FOR FVMPE-RS

• Manual Universal Turret Condenser, 8-position with built-in polarizer, for transmitted light brightfield, darkfield, phase contrast, polarized light and Nomarski Differential Interference Contrast.

• U-TLD;DRY TOP LENS UNIVERSAL CONDENSER, NA 0.90

• 32PO;32MM ROUND POLARIZER FOR VISIBLE TO 775NM

• U-POFW; 4PLACE 32FILTER WHEELE 2ROTATABLE/FILTER POCKET

• U-DIC10;NOMARSKI PRISM FOR 10X OBJ,0.9 TOP,UNIV CONDENSER

• U-DIC20;NOMARSKI PRISM FOR 20X OBJ, 0.9 TOP,UNIV CONDENSER

• U-AW-1-3; ATTENUATION WHEEL 6-POS,ND POS

• U-FFWADP;ADAPTER OF FILTER WHEEL ON LIGHT SOURCE

• U-IFCBL100; CONNECTION CABLE FOR IX3/BX3 - 100CM

• FV31-STBX; Adaptor to connect scanner and BX63L frame

• FV31-SD; FV3000 Standard 2CH PMT Detection unit

• Reverse spectral DM560

• FV31-MCOMB;FV3000 main laser AOTF combiner for up to 5

LDS

• FV31-FURBB;Broad band fiber unit 400-640nm for FV31-MCOMB

• FV3000 6CH laser diode power supply

• FV31-SCOMB;FV3000 Sub combiner for FV31-MCOMB

• FV31-CCFUR;FV3000 Fiber unit between MCOMB and SCOMB

• FV31-EXDM-MS;ExDM turret for Main and Sub laser combine

• FV31-ADPM;Adapter mirror for MCOMB with SCOMB

• FV31-ADP445;Laser diode adapter kit for LD445

• FV31-ADP514;Laser diode adapter kit for LD514

• 1FV31-DM-NEY;ExDM for 445/514/561

• FV30-PCIB;PCI-E CONFOCAL INTERFACE BOARD

• Assembled 405/488/561/640/730 excitation dichroic for FV3000

• 730 nm laser system for FV3000 ***Customized product 120 days lead time***

• V31-BCOMB;FV3000 Beam combining unit for additional lasers

• F FV31-AUXOUT; Universal analog output unit to control additional lasers

• MT-014; LOGO CLOTH DUSTCOVER 23x28x31, ANTISTATIC, FV

• ZDECK W/MOTO XY PLATFORM, 0.02um Res and 0.05um scales

• pE-300lite for upright FV3000. Includes pE-300 unit, 3m liquid light guide, and custom U-AW adapter

• ET-DAPI 395/25X, BS425, 460/50M w/IX3XL cube

• 49002-IX3; ET-EGFP/FITC/CY2 470/40X, BS495, 525/50M

• 49008-BX3; ET-MCHERRY/TXRED 560/40X BS585, 630/75M

• LASER DIODE 405NM,CW 50MW

• LASER DIODE 445NM,CW 75MW

• LASER DIODE 488NM,CW 20MW

• LASER DIODE 514NM,CW 40MW

• LASER DIODE 561NM,CW 20MW

• LASER DIODE 640NM,CW 40MW

• FV Workstation

• 163-SPECIAL; with M6 tapped holes 25mm OC STAINLESS TOP 750mm WIDE x 1050mm DEEP x

50mm thick

• 5-29-26; SLIM 5 RACK 29RU 20"DEEP

Brand Name Justification – Olympus upgrade to FV3000 3

Delivery:

The contractor shall deliver the instrument within 120 days after the award to: NCCIH, Bethesda, Maryland 20892.

The contractor that receives the award will be provided with the complete mailing address and point of contact.

Upon delivery of the award, the contractor must notify Sandy Putsky to schedule the delivery date and time.

Justification for this Brand Name acquisition is in accordance with FAR 13.501. The use of the Olympus FV3000 and associated components is essential to the Governments requirement, thereby precluding consideration of a product manufactured by another company.