(Beta.SAM.gov) Salient Characteristics.pdf

Salient Characteristics

Statement of Need The National Institute of Allergy and Infectious Diseases (NIAID) seeks to purchase a MALDI mass spectrometer for the Laboratory of Clinical Immunology and Microbiology (LCIM). The proposed purchase is for a Brand Name or Equal. The brand name and model number of the product is: Bruker Scientific LLC’s timsTOF flex LC-MS (Liquid Chromatography-Mass Spectrometer) system.

Salient Characteristics (Key Requirements) To be considered for award, offerors of “equal” products, including “equal” products of the brand name manufacturer, must meet the following criteria for the salient characteristics:

1) Acceptable size dimensions of no more than 275cm in length x 100 cm in width x 250 cm in height for the equipment, potential key add-ons, and other key components combined. These dimensions are hard limits due to the physical size constraints of the space.

2) Power requirements. No more than 2x 220V plugs due to the power constraints of the lab.

3) Imaging mass spectrometer. We require the ability to look at substances formed from metabolism in 2D space.

4) Trapped ION mobility techniques (TIMS). We require the ability to assess the substance’s mass and 3D shape in space (collisional cross section; CCS). Program needs to use this ability to distinguish between specific lipid residues. The equipment must be able to use the ION Mobility technique with the MALDI imager. The MS instrument must contain a trapped ion mobility spectrometry (TIMS) analyzer delivering high mobility resolution with parallel accumulation– these features allow for samples to be sorted by CCS prior to time of flight (TOF) analysis while the next allotment of sample is loaded. This will assure ION mobility can be performed quickly on the entire high throughput sample.

5) High resolution QTOF. QTOF analysis must also be high resolution (60000 full sensitivity resolution at m/z 1222) to assure a mass accuracy of <0.8ppm internally and 2ppm externally calibrated. Along with CCS from ION mobility, this will assure molecules are identified correctly.

6) First-pass identification. We require mass spectrometer capable of first-pass identification that doesn’t require follow up evaluations because we want to assess a large number of samples. This first pass ID ability via ION mobility techniques allows high throughput screening of at least 20 true square pixels per second.

First pass identification via ION mobility is required for high throughput that is needed for this project. We require the ability to run a lot of samples while being sure of the findings on the first run without requiring numerous second, more targeted runs relying on tandem mass spec.

7) Tandem mass spectrometry collision chamber. This ability is required because we need the ability to run additional identification if ION mobility failed to make an identification. A built-in option must be able to isolate ions with m/z values up to 3000 with a quadruploe mass range of up to 40,000 m/z.

8) MALDI/MALDI input. Instrument must have MALDI input to allow fast loading and processing of samples - otherwise there is no way to get high throughput and no way to get MALDI imaging. MALDI input must allow for up to 1536 positions of analysis in a microtiter plate format to maximize throughput. We anticipate running numerous samples under numerous conditions and maximizing positional possibilities will allow for large number of samples to be included in each run.

9) MALDI imager with a square laser. We require a MALDI imager with a square laser to avoid oversampling, which causes uncertainty in identification and localization. The use of square lasers prevents overlap in the area being sampled, in comparison to circular lasers that must overlap in order to cover an entire image area. The equipment must be able to use the ION Mobility technique with the MALDI imager.

10) MALDI imager laser that is 20 micrometers or smaller. The MALDI laser imaging area must be 20 micrometers or smaller because larger lasers would not have the resolution required to analyze certain fine-grain details on the tissues and monolayer cell cultures.

11) MALDI imager laser must be a 10kHz laser or faster. Anything slower will create delays that are beyond the tolerance for the high throughout requirements of the project.

Imager must be able to run at least 10kHz at the size of 20 micrometers or smaller spatial area so that the combination of high-speed and high-resolution can be achieved.

12) Ability to immediately swap between MALDI and ESI (direct injection) to allow immediate calibration between the inputs (preventing any delays) and allow collaborators to share in the ability of the device.