B SBSS 75N95022Q00125.pdf

SOURCES SOUGHT NOTICE

1. Sources Sought Number: 75N95022Q00125

SAM.gov: https://sam.gov/opp/9ef8427c8269463792972de0d710da04/view

2. Title: Agilent 6546 QTOF LC-MS System with accessories (Brand-name or equal).

3. Classification Code: 6640 - Laboratory Equipment and Supplies

4. NAICS Code: 334516 - Analytical Laboratory Instrument Manufacturing

5. Description:

This is a Small Business Sources Sought notice. This is NOT a solicitation for proposals, proposal abstracts, or quotations. The purpose of this notice is to obtain information regarding: (1) the availability and capability of qualified small business sources; (2) whether they are small businesses; HUBZone small businesses; service-disabled, veteran-owned small businesses; 8(a) small businesses; veteran-owned small businesses; woman-owned small businesses;

or small disadvantaged businesses; and (3) their size classification relative to the

North American Industry Classification System (NAICS) code for the proposed acquisition. Your responses to the information requested will assist the

Government in determining the appropriate acquisition method, including whether a set-aside is possible. An organization that is not considered a small business under the applicable NAICS may submitted a response to this notice.

This notice is issued to help determine the availability of qualified companies technically capable of meeting the Government requirement and to determine the method of acquisition. It is not to be construed as a commitment by the

Government to issue a solicitation or ultimately award a contract. Responses will not be considered as proposals or quotes. No award will be made as a result of this notice. The Government will NOT be responsible for any costs incurred by the respondents to this notice. This notice is strictly for research and information purposes only.

Background: The National Institutes of Health (NIH) is the nation’s leading medical research agency and the primary Federal agency whose mission is to seek fundamental knowledge about the nature and behavior of living systems and the application of that knowledge to enhance health, lengthen life, and reduce illness and disability, conducting, supporting and making medical discoveries that improve people’s health and save lives.

https://sam.gov/opp/9ef8427c8269463792972de0d710da04/view

NCATS is one of 27 Institutes and Centers at the NIH and its mission is to catalyze the generation of innovative methods and technologies that will enhance the development, testing and implementation of diagnostics and therapeutics across a wide range of human diseases and conditions. The NCATS DPI plans, conducts and uses both internal and contract resources to advance collaborative research projects across the preclinical phases of the translational science spectrum.

The NCATS DPI IFX enables data-driven decisions that accelerate translation by developing new or enhancing the application of existing methods that prioritize targets and therapeutic opportunities for evaluation; designing novel, fit-for-purpose chemical probes; and assessing the productivity of the translational research process.

The NCATS DPI Analytical Chemistry Core (ACC) maintains a state-of-the-art laboratory whose mission is to support translational research throughout NCATS by providing analytical chemistry services involving the isolation, purification, identification and analysis of various therapeutic modalities for targeted disease states.

Untargeted metabolomic profiling captures a detailed view of the chemical processes underlying cells and is the closest representation of a given phenotype (compared to other omics). Untargeted metabolomics thus plays a key role in translational research, from the identification of biomarkers to an enhanced understanding of the mechanisms that underlie diseases, and to the identification of targetable pathways or molecules.

Importantly, untargeted approaches pinpoint specific pathways or reactions that can be monitored through high-throughput targeted assays. An ultimate goal is to automate the process of generating, analyzing, and interpreting untargeted metabolic profiles to accelerate research efforts.

The acquisition of an ultra-performance liquid chromatography-quadrupole time-of-flight mass spectrometry (UPLC/QTOF) system dedicated to untargeted metabolomics would expand mass spectrometry capabilities within DPI providing significant benefit by enabling NCATS to meet the support demands of both internal and external collaborators. Current mass spectrometry instrumentation in the NCATS DPI ACC is primarily configured for routine targeted MS-based studies. By having access to a

UPLC-MS dedicated to untargeted omics experimentation, NCATS will be able to fulfill unmet research needs. Multiple applications of this requested instrumentation at NCATS would include 1) identifying biomarkers of toxicity, treatment response, and diseases; 2) elucidating biological mechanisms underlying cellular differentiation, disease development, and treatment response; 3) narrowing down possible compounds to evaluate in assays based on metabolism features. This instrument would be part of a broader NCATS effort, that includes ACC, Early Translation Branch (ETB), Therapeutic

Development Branch (TDB), Chemical Genomics Branch (CGB), and Information

Technology Resources Branch (ITRB), to leverage metabolomics and other omics in preclinical and clinical research. It is worth noting that untargeted and targeted MS-based methods dovetail together to enable meaningful discoveries and facilitate comprehensive analysis throughout the progression of projects.

Purpose and Objectives: The Informatics (IFX) Core Group, within the Division of

Preclinical Innovation (DPI) at the National Center for Advancing Translational Sciences

(NCATS) seeks to purchase a Liquid Chromatography High-Resolution Quadrupole

Time-of-Flight (QTOF) with Tandem Mass Spectrometer (LC-MS/MS) in order to expand mass spectrometry capabilities and fulfill unmet research need.

Project Requirements: For reference purposes, the Liquid Chromatography High-

Resolution Quadrupole Time-of-Flight (QTOF) with Tandem Mass Spectrometer (LC-

MS/MS) that meet the agency need is the Agilent 6546 Quadrupole Time-of-Flight MS

Instrument with 1290 Infinity II LC (Brand-name or equal).

See the attached Purchase Description for the complete list of brand-name items, descriptions, and salient characteristics.

Contract Type and Anticipated Period of Performance / Delivery Date: The

Government anticipates awarding a firm fixed-price contract under a future solicitation.

The anticipated delivery date is three (3) weeks after receipt of order, with installation and training to occur as soon as practicable after delivery on dates and times agreed upon by NCATS and the contractor. Installation and training may or may not occur on the same date.

Capability statement /information sought. Respondents must provide clear and convincing documentation of their capability of providing the products specified in this sources sought notice.

Moreover, interested vendors must provide a capability statement for:

• the difficulty and/or feasibility of the potential requirement or proposed acquisition, possible solutions and approaches that may currently exist in the marketplace, and information regarding innovative ideas or concepts; and

• information regarding respondents’: (a) staff expertise, including their availability, experience, and formal and other training; (b) current in-house capability and capacity to perform the work; (c) prior completed projects of similar nature; (d) corporate experience and management capability; and (e) examples of prior completed Government contracts, references, and other related information.

• Written capability statements should demonstrate your organization’s capability to provide the brand-name or equal items listed in the Project Requirements section above. If the entity believes to be an authorized resellers of the brand-name product specified in this announcement, the entity’s capability statement must include: (a) product, catalog, model, and/or part number(s); (b) product description; (c) all relevant information and documentation that the item(s) offered meets the salient physical, functional, or performance characteristics as specified in the Project Requirements section above.

The respondent must also provide their DUNS number, organization name, address, point of contact, GSA Schedule (if any), and size and type of business (e.g., 8(a), HubZone, etc., pursuant to the applicable NAICS code and any other information that may be helpful in developing or finalizing the acquisition requirements.

Submission Instructions:

One (1) copy of the response is required and must be in Microsoft Word or Adobe PDF format using 11-point or 12-point font, 8-1/2” x 11” paper size, with 1” top, bottom, left and right margins, and with single or double spacing.

The information submitted must be in and outline format that addresses each of the elements of the project requirement and in the capability statement /information sought paragraphs stated herein. A cover page and an executive summary may be included but is not required.

The response is limited to ten (10) pages. The 10-page limit does not include the cover page, executive summary, or references, if requested.

The response must include the respondents’ technical and administrative points of contact, including names, titles, addresses, telephone and fax numbers, and e-mail addresses.

All responses to this notice must be submitted electronically to the Contract Specialist.

Facsimile responses are NOT accepted.

Responses must be received by 9:00 a.m., Eastern Time, on March 4, 2022, and reference Sources Sought Number 75N95022Q00125. Responses must be submitted by email to Renato Gomes, Contract Specialist, renato.gomes@nih.gov.

“Disclaimer and Important Notes: This notice does not obligate the Government to award a contract or otherwise pay for the information provided in response. The

Government reserves the right to use information provided by respondents for any purpose deemed necessary and legally appropriate. Any organization responding to this notice should ensure that its response is complete and sufficiently detailed to allow the

Government to determine the organization’s qualifications to perform the work.

Respondents are advised that the Government is under no obligation to acknowledge receipt of the information received or provide feedback to respondents with respect to any information submitted. After a review of the responses received, a presolicitation synopsis and solicitation may be published in Federal Business Opportunities. However, responses to this notice will not be considered adequate responses to a solicitation.

mailto:renato.gomes@nih.gov

Confidentiality: No proprietary, classified, confidential, or sensitive information should be included in your response. The Government reserves the right to use any non-proprietary technical information in any resultant solicitation(s).”

Sources Sought Number: 75N95022Q00125

Purchase Description

For reference purposes, the Liquid Chromatography High-Resolution Quadrupole Time-of-

Flight (QTOF) with Tandem Mass Spectrometer (LC-MS/MS) that meets the need of this requirement is the Agilent 6546 Quadrupole Time-of-Flight MS Instrument with 1290 Infinity II

LC, as follows:

Line Item No.

Part No. Item Description Qty

1 G6546AA

Agilent 6546 Q-TOF LC/MS System with Agilent Jet Stream source, oil foreline pump, bundled PC, monitor, installation (44K), familiarization (44L), credits for a 4-day Agilent training class where available, and one (1) Year SW Update/Phone Assist (44W)

2 M5960AA

MassHunter Workstation Software for LC/TOF and LC/(Q)TOF with installation (44K), familiarization (44L), and one (1) Year SW Update/Phone Assist (44W). This software includes feature extraction and data quality control.

3* G7120A

1290 Infinity II High Speed Pump. Power range 1300 bar and 5ml/min flow, binary high pressure mixing, lowest delay, highest precision and accuracy. Includes Active Seal Wash, Tool Kit, Solvent Cabinet, bottles and ISET.

4* G7167B

1290 Infinity Multisampler up to 1300 bar for well plates and individual sample containers. Includes 1 sample drawer, 2x 54-vial containers, a needle flush port, 40 uL metering device, 20 uL loop. (for Dual-needle instruments metering is 100 ul and loop size is 20 ul) with installation (44K).

5* G7116B

1290 Infinity II Multicolumn Thermostat. Capacity up to 8 columns, temperature range 4 deg C. to 110 deg C. Includes 1.6uL Quick-Connect Heat Exchanger, Quick-Connect fitting and two Quick-Turn fittings, with Installation (44K).

6* G4231C 2 pos/6 port valve head, 1300 bar with installation (44K) 1

7 G3835AA

Mass Profiler Professional (MPP) as well as installation (44K), familiarization (44L), and one (1) Year SW Update/Phone Assist (44W). This software includes statistical analyses and intuitive visualization tools (e.g., uni- and multi-variate analyses, PCA, clustering, class prediction)

8 G3836AA

Pathway Features for MPP with familiarization (44L), and one (1) Year SW Update/Phone Assist (44W) This software enables pathway enrichment analyses.

9 G3874AA

MassHunter METLIN Metabolite PCDL. Personal Compound Database and Library for ~25000 endogenous metabolites, with ~ 9000 accurate mass MS/MS spectra for over 2300 compounds and RTs for 700 compounds

10 G3876CA

Standalone Forensics Tox PCDL library (contains drugs of abuse).

11 G5883CA

Standalone Mycotoxin Personal Compound Database and Library for current Agilent TOF and Q-TOF LC/MS Systems

12 G6890CA

Extractables and Leachables Accurate Mass PCDL for Agilent LC/MS systems

13 H2149A Method and Application Consulting On-site consulting for a maximum of four (4) participants for five (5) day on-site (includes travel)

14 5043-1759 Flex Bench, Floor with PS 1

15 5043-1735 Drawer for Flex Benches 1

* In addition to the item description provided above, the items listed herein have the following configuration:

Item 3: G7120A with the following configuration:

a. High Speed UCT (033)

b. Jet weaver upgrade kit (070)

c. Prooshell 120 EC-C18,2.1x50mm, 1.9 um

d. Manual DVD for 1220/1260/1290

e. Agilent Lab Advisor Advanced Software

f. Ultra Clean Tubing Kit

g. A-Line Stay Safe Cap Starter Kit

h. V380 Jet Weaver Mixer Upgrade Kit

Item 4: G7167B with the following configuration:

a. MassHunter System Driver

b. Agilent InfinityLab sample thermostat

c. Dual-height drawer kit

d. 1290 Infinity multi-wash option

Item 5: G7116B with the following configuration:

a. Quick-Conn HE ULD (064)

b. Valve drive for 1290 MCT

c. Quick-Connect HE Standard Flow

d. Quick-Connect HE Ultra Low Dispersion

e. Column ID kit for 1290 Infinity II MCT

Item 6: G4231C with the following configuration:

a. Kap kit QCHE G4231C

b. 2/6 cap kit 0.12 mm ID, incl. QC-HE

The salient characteristics of the Liquid Chromatography High-Resolution Quadrupole Time-of-

Flight (QTOF) with Tandem Mass Spectrometer (LC-MS/MS) include:

• Automated optimization of ion optics and full QTOF mass axis calibration, fully integrated part of the mass spectrometer (no manual adjusting of plumbing).

• Transparent and automated introduction of reference masses through a built-in calibrant delivery system with support for real-time internal reference mass correction for MS and MS/MS operation.

• Up to 5 orders of dynamic range within a single spectrum containing co-eluting compounds.

• Analog to Digital Conversion at a minimum of 10 GHz.

• <1ppm mass accuracy within a 3-degree Celsius drift per hour without needing to re-tune, to accommodate temperature variation in the laboratory. Active temperature compensation.

• For maximum uptime and robustness, ion sources are single bend 90-degree orthogonal geometry, in order to minimize chemical noise, carryover, maintenance, and ion suppression.

• Isolation valve included for vent-free capillary maintenance, preventing unnecessary downtime during routine operation.

• Focusing of the electrospray plume at the sampling aperture of the mass spectrometer for optimal ion sampling and peak shape.

• Detection and enhancement signal of fragile molecules that are susceptible to fragmentation and loss within the atmospheric pressure interface of an LCMS.

• Data Dependent MS/MS and Data Independent MS/MS support.

• Options to add on the following additional sources: APCI, APPI, combined ESI/APCI, nanoESI interface, and GC APCI source with fully integrated GC and transfer line control.

• TOF mass resolution of at least 30,000 at m/z 200 and 60,000 at m/z 2,722 after automatic tuning procedure, at full acquisition speed, with a single bend V-shaped flight path.

• Mass measurement accuracy <0.8 ppm RMS error in TOF mode as measured by calculating average error from 10 repeat injections onto an HPLC column under electrospray ionization conditions.

• Mass measurement accuracy <2 ppm RMS error in MS/MS mode as measured by calculating average error from 10 repeat injections onto an HPLC column under electrospray ionization.

• MS sensitivity of 1 pg of reserpine on column under electrospray ionization mode at HPLC flow rate of 400 uL/min with a signal-to-noise ratio of at least 500:1 for the reserpine (M+H)+ signal at m/z 609.2807. Must be achievable while maintaining maximum mass resolution.

• MS/MS sensitivity of 1 pg of reserpine on column under electrospray ionization mode at HPLC flow rate of 400 uL/min with a signal-to-noise ratio of at least 1500:1 RMS for the most intense product ions (m/z 174, 195, 397, 448). Achievable while maintaining maximum mass resolution.

• Resolving power specification (60,000 at 2,722m/z) and simultaneous sensitivity specification shown above for 1 pg of reserpine on column under electrospray ionization mode at HPLC flow rate of 400 uL/min. Both of these criteria must be met under the same instrument conditions – intent is to avoid sacrificing sensitivity or resolution to optimize the other criteria.

• Spectral acquisition rate of 50 spectra/second in MS mode and 30 spectra/second MS/MS mode while maintaining a resolution of 60,000 at m/z 2,722.

• Isotopic fidelity such that the relative isotopic abundance error is less than 5% of the RMS for 10 ESI calibrant ions in positive polarity (RIA error (%) = 100 × (RIAexp – RIAtheo)/ RIAtheo).

• Handling and Shipping to NCATS, FOB Destination.

• Installation and Equipment familiarization on-site (NCATS).

• On-site consulting for at least four (4) participants for five (5) day (includes travel).

• Twelve (12) month warranty on the instruments.

• Ninety (90) days warranty on consumables.

• Twelve (12) months software updates and phone assistance.