Attch 1._PD.pdf

1. Background Information and Objective:

Cryopreservation is the use of very low temperatures to preserve and store intact living cells and tissue. This is of particular importance for clinical and research laboratories that require the safe storage of both clinical (patient) and research samples. These include patient tissue, cell lines, and samples obtained from in vivo research projects. These extreme temperatures (-150°C) allow researchers to safely store these invaluable samples and access them at a later time.

These extremely cold temperatures preserve both their viability and genetic material without compromising integrity over time. Cells (both cell lines and cell suspensions acquired from clinical and research samples) and tissue can be stored for many years without compromising viability or genetic profiles.

The Azenta A440 Liquid Nitrogen Freezer is a high-capacity storage system designed to safely preserve biological samples at extremely low temperatures. In the required 13-2 Square Rack configuration, it can store up to 41,600 2 mL vials and maintain samples below the required temperature threshold for more than 15 days in the event of a disruption in normal operation.

This acquisition is for one (1) Cryogenic LN2 Vapor Cryo Freezer and associated racks for the proper long-term storage of clinical and research samples utilized by the clinical and research staff of the Pediatric Oncology Branch (POB), National Cancer Institute (NCI), National Institutes of Health (NIH).

2. Generic Name of Product:

Liquid Nitrogen (LN₂) Vapor-Phase Cryogenic Storage Freezer with Racks (Qty. 32)

3. Purchase Description: The unit is designed to maximize storage while fitting into limited lab space. It provides more capacity without requiring additional floor space, allowing it to replace existing freezers without needing layout changes. The freezer is easy to use and includes a touchscreen that automatically maintains the correct temperature and liquid nitrogen levels. It also records important data about the freezer’s performance, which can be saved, emailed, or uploaded to the cloud. Built-in WiFi/LAN allows users to monitor the system remotely and receive alerts if there are any issues. To help protect samples, the system continuously monitors both temperature and liquid nitrogen levels independently.

Features like interior LED lighting and automatic fog clearing improve visibility, making it easier to locate and access samples. The design also focuses on user safety and convenience, with features like a lower access height and a fold-out step to make reaching samples easier and reduce strain. Overall, the Azenta A440 provides a reliable, space-efficient, and user-friendly solution for long-term sample storage in a laboratory setting.

4. Salient characteristics:

- 13-2 Square Rack Configuration: 41,600 x 2 mL vials | 416 x 2-inch boxes | 32 racks

- Outer Diameter: 45” (1143mm)

- Overall Height: 59” (1499mm)

PURCHASE DESCRIPTION

Cryogenic LN2 Vapor Cryo Freezers and Racks

Azenta A440

- Usable Height: 30” (762mm)

- Step Height: 10.5” (267mm)

- Liftover Height: 39.5” (1003mm)

- LN2 Capacity: 150 L

- Hold Time (< Tg): > 15 Days

- Optimized design for size/capacity/usability

- Touchscreen controller automatically maintains freezer conditions

- Critical data is recorded (can be pushed to the cloud, emailed or saved to USB drive)

- Supports independent temperature and liquid nitrogen level monitoring and control

- Increased capacity while maintaining footprint size of current units to optimize restricted space

- Cryogenic LED and automatic fog-clear to enhance visibility and sample access

- Improved ergonomics allowing for safer/easier access to samples

- Cryogenic freezers must fit in space currently occupied by the units being replaced as floor space is limited.

- Warranty: 3 year parts and craftsmanship | 7 year vacuum insulation

5. Quantity:

One (1) - A440 LN2 Vapor Freezer Thirty-two (32) – 13-2 Square Vial Box Racks

6. Delivery Date: 60 days ARO

Delivery Address/Installation:

Attention: Miki Kasai, Technical Laboratory Manager Bldg10CRC, 1W-3833 10 Center Drive Bethesda, MD. 20892

(240) 858-3407

Version 11-30-2016-1

FAR Part 11—Describing Agency Needs