Attachment-SOW-Covid-19 Inflammatory Analysis.pdf

Version 11-30-2016-1 FAR Part 11—Describing Agency Needs

STATEMENT OF WORK (SERVICES)

(SOW)

GENERAL INFORMATION

Title of Project:

COVID-19 inflammatory analysis of a racially diverse cohort

Statement of Need and Purpose:

The contractor shall provide all resources necessary to quantify the levels of 10 cytokines and 4 chemokines in serum from HANDLS participants.

Background Information and Objective:

The DNA Repair Unit (DRU) is examining factors that influence the severity of COVID-19 infection and the inflammatory state in a racially diverse cohort. Given our preliminary data, we hypothesize that inflammatory markers vary among individuals with different races and socioeconomic status with relation to COVID-19 infection. We predict that we will identify inflammatory signatures with race and COVID-19 infection in the HANDLS cohort. Information gained from this analysis will be important for assessing risk for morbidity and long-term consequences due to COVID-19 in this cohort.

Period of Performance:

July 1, 2021 to June 30, 2022

SCOPE OF WORK

General Requirements:

Independently and not as an agent of the Government, the Contractor shall furnish all the necessary services, qualified personnel, material, equipment, and facilities, not otherwise provided by the Government as needed to perform the Statement of Work below:

Specific Requirements:

Contractor shall quantitatively analyze the levels of all 10 cytokines (IFN-g, IL-1b, IL- 4, IL-5, IL-6, IL-8,IL- 10, IL-12p70, IL-22, TNFa) and 4 chemokines (IP-10, ITAC, MCP-1, MIP- 3b) in human serum. Limited volumes (40 microliters) are required to assay these proteins. This is critical since HANDLS serum samples are limited and have high research significance. A multiplex system is required to analyze all these proteins in the minimal amount of serum required. The vendor shall analyze the 14 different human biomarkers using the Simoa® Planar Array Technology and SP-X™ imaging and analysis platform to allow us to compare these data with other generated datasets using this type of technology. A total of eight 96-well plates will be run for all 14 proteins. The contractor will provide a certificate of quality, and a data report with quantities of each of the 14 proteins for each sample in an electronic data file along with information on quality controls.

Version 11-30-2016-1 FAR Part 11—Describing Agency Needs

LEVEL OF EFFORT:

Not Applicable.

GOVERNMENT RESPONSIBILITIES

The government will provide the deidentified human serum samples, each with a unique id.

DELIVERY OR DELIVERABLES

The contractor will provide a certificate of quality, and a data report with quantities of each of the 14 proteins for each sample in an electronic data file along with information on quality controls.

REPORTING REQUIREMENTS

OTHER CONSIDERATIONS

Travel:

Travel is not required.

Key Personnel:

Information System Security Plan:

Data Rights:

Section 508—Electronic and Information Technology Standards:

Publications and Publicity:

Confidentiality of Information: