Attachment-PURCHASE DESCRIPTION Metabolic Analyzer.pdf

Version 11-30-2016-1 FAR Part 11—Describing Agency Needs

PURCHASE DESCRIPTION

1. Statement of Need and Purpose: The Translational Gerontology Branch (TGB) is in need of a Metabolic Analyzer for live metabolic phenotyping.

2. Background Information and Objective: A better understanding of the metabolic changes occurring in aging under normal or diseased condition can help improve/ accelerate the discovery of pharmacological and environmental interventions able to slow or arrest the body decline. The Seahorse XFe96 Analyzer supports the non-invasive real time measurement of glycolysis and mitochondrial respiration and /or fatty acid oxidation in live cells, tissue fragments and organoids. The Seahorse Technology supports a wide range of applications within the IRP biomedical research including oncology, infectious disease, musculoskeletal, cardiovascular, neurology, respiratory and stem cell research.

3. Generic Name of Product: Metabolic Analyzer

4. Purchase Description: Seahorse XFe96 Analyzer (64-Bit) Hardware (PART# S7800B) Including: Wave SW software, XF Imaging and Normalization, shipping, professional installation and specialized training at installation.

5. Salient characteristics:

1. Simultaneously measures oxygen consumption rate (OCR) and extracellular acidification rate (ECAR) ofliving cells/tissues.

2. Non-invasive measurement of glycolysis, mitochondrial respiration and fatty oxidation requires no addition of dyes, labels or reporters.

3. Cells/microplate remain viable following an Agilent Seahorse XF assay and may be used for another assay.

4. OCR and ECAR rate are calculated in 2-5 minutes using data acquired every 14 seconds.

5. Measurements may be repeated indefinitely to measure kinetic responses with rates reported every 5-8 min.

6. Measures adherent as well as suspension cells, organoids, or tissue (i.e pancreatic islets).

7. Up to four test compounds may be added automatically to each well during the assay.

8. Measurements may be performed before and after each compound is added.

9. Temperature controlled measurement chamber is maintained at 37°C ± 0.5°C.

Version 11-30-2016-1 FAR Part 11—Describing Agency Needs

10. Optical sensors do not consume oxygen during the measurement and are not in contact with the cells, nor are affected by compound or intracellular dye fluorescence.

11. Oxygen sensor peak absorption = 530 nm (green) and Oxygen peak emission = 650 nm (red).

12. pH sensor peak absorption = 470 nm (blue) and pH sensor peak emission = 530 nm (green).

13. Light Emitting Diodes (LEDs) used as the monochromatic excitation source at very low excitation energy densities to prevent detectable levels of photobleaching.

14. Data display shows OCR and ECAR data simultaneously.

15. Software provides integrated data analysis, and data can easily be exported into MS® Excel for transfer into other statistical analysis programs.

6. Quantity: (1) Seahorse XFe96 Analyzer (64-Bit) Hardware (PART# S7800B) Including: Wave SW software, XF Imaging and Normalization, shipping, professional installation and specialized training.

7. Delivery Date: 21 days after receipt of order

8. Period of Performance: N/A

9. Option(s): None