Attachment One Scopes of Accreditation 12639521Q0082.pdf

SCOPES OF ACCREDITATION TO ISO/IEC 17025:2017

NATIONAL VETERINARY SERVICES LABORATORIES

USDA-APHIS-VS-NVSL

DIAGNOSTIC BACTERIOLOGY AND PATHOLOGY LABORATORY

DIAGNOSTIC BIOANALYTICAL AND REAGENT LABORATORY

DIAGNOSTIC VIROLOGY LABORATORY

1920 Dayton Avenue Ames, Iowa 50010

BIOLOGICAL SCOPE - 1

Test Type/Platform Test Method(s)

Diagnostic Bacteriology and Pathology Laboratory – Bacterial Identification

Agar Gel Immunodiffusion Pasteurella multocida SOP-BI-0036 Taylorella spp. SOP-BI-0106

Agglutination

Francisella tularensis SOP-BI-0207 Leptospira (Microagglutination) SOP-BI-0038 Mycoplasma SOP-BI-0079 Mycoplasma (Hemagglutination inhibition) SOP-BI-0078 Salmonella SOP-BI-0021, SOP-BI-0022 Salmonella Pullorum and Typhimurium SOP-BI-0015, SOP-BI-0016

SOP-BI-0017

Antibiotic Susceptibility

Various bacterial isolates SOP-BI-0010

Biochemical Testing

Various bacterial isolates SOP-BI-0053, SOP-BI-0075 Bacillus anthracis SOP-BI-0202 Burkholderia mallei and pseudomallei SOP-BI-0204 Francisella tularensis SOP-BI-0205 Salmonella SOP-BI-0021, SOP-BI-0022, SOP-BI-0031

Fluorescent Antibody

Francisella tularensis SOP-BI-0206 Taylorella spp. SOP-BI-0107

MALDI-Biotyper

Various bacterial isolates SOP-BI-0075, SOP-BI-0115

Microscopic Examination (staining) Various bacterial isolates SOP-BI-0073 Bacillus anthracis SOP-BI-0202 Burkholderia mallei and pseudomallei SOP-BI-0204

Real-time PCR

Taylorella spp. SOP-BI-0109

Sequencing 16S rDNA Various bacterial isolates SOP-BI-0065, SOP-BI-0129

Solid Media (Various Medias)

Various bacterial isolates SOP-BI-0075 Bacillus anthracis SOP-BI-0200, SOP-BI-0201, SOP-BI-0202, SOP-BI-0204

Francisella tularensis SOP-BI-0205 Salmonella SOP-BI-0021, SOP-BI-0022

Diagnostic Bacteriology and Pathology Laboratory – MB

Bacterial Isolation Brucella spp. SOP-MB-0009 Mycobacterium spp.

Culture SOP-MB-0039, SOP-MB-0059

SOP-MB-0022, SOP-MB-0041

SOP-MB-0042, SOP-MB-0045

Solid Media SOP-MB-0040, SOP-MB-0057

Phenotypic Identification Mycobacterium spp.

Microscopy (Acid-fast Staining) SOP-MB-0028, SOP-MB-0037

Molecular Identification Brucella spp.

Confirmatory PCR - AMOS SOP-MB-0017, SOP-MB-0019 Mycobacterium spp.

Direct PCR - M. bovis, MAP, MTBC SOP-MB-0046, SOP-MB-0079, SOP-MB-0080

Confirmatory PCR - MTBC Differential, MAP SOP-MB-0052 Sequencing - 16s rDNA ITS, rpoB, hsp65 SOP-MB-0031

Genomic Characterization

Microsatellite Bovine Identity Testing SOP-MB-0060 Ovine Identity Testing SOP-MB-0076

Sequencing Scrapie Susceptibility SOP-MB-0074

Whole Genome Sequencing Nextera – TB complex and Brucella spp. SOP-MB-0075, SOP-MB-0085

Diagnostic Bacteriology and Pathology Laboratory – Pathology

Microscopic Examination Bovine Spongiform Encephalopathy SOP-PS-0037 Mycobacterium spp. SOP-PS-0045 CWD/Scrapie SOP-PS-0002

ELISA

Bovine Spongiform Encephalopathy SOP-PS-0003 Chronic Wasting Disease SOP-PS-0007 Scrapie SOP-PS-0006

Histopathology Staining

Auromine O and Acridine Orange Stain SOP-PS-0025 Congo Red Stain SOP-PS-0014 Giemsa Stain SOP-PS-0027 Gram Stain SOP-PS-0021, SOP-PS-0005 Grocott’s Methanamine Silver Stain REF-PS-0022 Hematoxylin and Eosin Stain SOP-PS-0024 Luxol Fast Blue Stain SOP-PS-0012 Modified Ziehl-Neelson Stain SOP-PS-0026 Periodic Acid-Schiff Stain SOP-PS-0020 Toluidine Blue O Stain SOP-PS-0013

Immunohistochemistry (IHC)

Bovine Spongiform Encephalopathy SOP-PS-0034 Scrapie SOP-PS-0002, WI-PS-0031 Chronic Wasting Disease

PCR

Mycobacterium spp. SOP-PS-0054

Western Blot TeSeE Bio-Rad Kit (BSE, CWD, Scrapie) SOP-PS-0031

Diagnostic Bioanalytical and Reagent Laboratory – CAS

Agar Gel Immuno-double Diffusion (AGID) Species Verification SOP-CAS-0105

Individual Animal Identification SOP-CAS-0109

Diagnostic Bioanalytical and Reagent Laboratory – Parasitology

Microscopic Examination Hemoparasites – Anaplasmosis SOP-PARA-0011 Hemoparasites – Dourine Hemoparasites – Eperythrozoonosis Hemoparasites – Bovine Piroplasmosis Hemoparasites – Equine Piroplasmosis

Identification Ectoparasites – Fluke Ectoparasites – Larvae Ectoparasites – Lice Ectoparasites – Maggots Ectoparasites – Mange Ectoparasites – Mites Ectoparasites – Myiasis Ectoparasites – Scabies Ectoparasites – Screwworm Ectoparasites – Tapeworm

Diagnostic Bioanalytical and Reagent Laboratory – Serology

Johne’s

Mycobacterium avium, subsp. Paratuberculosis

Brucellosis

Brucella abortus/suis (Tube) B. abortus/suis (Plate) B. abortus/suis (BAPA) B.

abortus/suis (Rivanol) B.

abortus/suis (Card) B. canis (ME Tube) B. melitensis (Card)

Equine paratyphoid Salmonella abortus '

Complement Fixation Test

Anaplasmosis Anaplasma marginale

Bovine babesiosis Babesia bigemina & Babesia bovis

Brucellosis Brucella abortus/suis

Contagious Equine Metritis Taylorella equigenitalis

SOP-PARA-0008, SOP-PARA-0007

SOP-PARA-0004

SOP-SERO-0045

SOP-SERO-0019

SOP-SERO-0016

SOP-SERO-0017

SOP-SERO-0018

SOP-SERO-0020

SOP-SERO-0024

SOP-SERO-0027

SOP-SERO-0009

SOP-SERO-0010

SOP-SERO-0013

SOP-SERO-0015

SOP-SERO-0039

Dourine Trypanosoma equiperdum SOP-SERO-0006

Glanders Burkholderia mallei SOP-SERO-0008

Johne’s Mycobacterium avium subsp. Paratuberculosis SOP-SERO-0012

Piroplasmosis Babesia caballi & Theileria equi SOP-SERO-0003

Dual Path Platform (Lateral Flow) Tuberculosis

Mycobacterium bovis SOP-SERO-0044

ELISA

Anaplasmosis

Anaplasma marginale SOP-SERO-0029 Brucellosis

Brucella abortus/melitensis SOP-SERO-0023 B. ovis SOP-SERO-0035

Equine Piroplasmosis B. caballi & T. equi (Kit) SOP-SERO-0032

Fluorescence Polarization Assay (FPA)

Brucella abortus/suis (Tube) SOP-SERO-0031

Gamma Interferon Test Tuberculosis

Mycobacterium TB Complex (Bovigam) SOP-SERO-0051

Indirect Fluorescent Antibody

Babesia caballi & Theileria equi SOP-SERO-0004

Babesia caballi SOP-SERO-0040

Diagnostic Virology Laboratory

Virus Isolation Avian Influenza Newcastle Disease

SOP-AV-0018

Avian Adenovirus SOP-AV-0019, SOP-AV-0022 Avian Pox Avian Reovirus Infectious Bursal Disease SOP-AV-0018, SOP-AV-0019 Infectious Laryngotracheitis Vesicular Stomatitis

SOP-BPA-0005

Isolation of Viral Agents from Finfish in Tissue Cell Culture SOP-BPA-0014 Swine Influenza Virus SOP-BPA-0023

Encephalomyocarditis Virus SOP-BPA-0024 Hemagglutinating Encephalomyelitis Virus Porcine Circovirus Porcine Parvovirus Porcine Reovirus Porcine Respiratory Coronavirus Porcine Rotavirus Porcine Rubularvirus Porcine Sapeloirus (Enterovirus A) Porcine Teschovirus Transmissible Gastroenteritis Virus Viral Abortion (Porcine) Pseudorabies Virus SOP-BPA-0021, SOP-BPA-0024 Porcine Epidemic Diarrhea SOP-BPA-0024, WI-BPA-0041 Porcine Deltacorona Virus Porcine Reproductive and Respiratory Syndrome SOP-BPA-0045 Bovine Coronavirus SOP-BPA-0046 Bovine Enterovirus Bovine Herpesvirus 1 (Infectious Bovine Rhintracheitis) Bovine Herpesvirus 2 (Bovine Herpes Mammillitis) Bovine Herpesvirus 4 Bovine Papular Stomatitis Bovine Parvovirus Bovine Respiratory Syncytial Virus Bovine Rotavirus Bovine Viral Diarrhea Pseudocowpox Viral Abortion (Bovine) Bluetongue SOP-EO-0008 Epizootic Hemorrhagic Disease SOP-EO-0009 Equine Viral Arteritis SOP-EO-0010 Eastern Equine Encephalomyelitis SOP-EO-0011 Western Equine Encephalomyelitis Venezuelan Equine Encephalomyelitis West Nile Virus Equine Influenza SOP-EO-0012 Equine Adenovirus SOP-EO-0013 Equine Herpesvirus 1 Equine Herpesvirus 2 Equine Herpesvirus 3 Equine Herpesvirus 4 Equine Rhinitis Virus African Horse Sickness SOP-EO-0014

Isolation / Indirect Fluorescent Assay (IFA)

Chlamydophila psittaci SOP-AV-0020

Indirect Fluorescent Assay (IFA) Avian Encephalomyelitis SOP-AV-0026, SOP-AV-0050 Infectious Laryngotracheitis Avian Metapneumovirus Avian Pox Avian Reovirus Psittacine Herpesvirus

Bovine Coronavirus SOP-BPA-0047 Bovine Herpesvirus 4 Bovine Papular Stomatitis Bovine Parvovirus Bovine Rotavirus Porcine Reovirus Porcine Rotavirus Pseudocowpox Porcine Reproductive and Respiratory Syndrome SOP-BPA-0031 Porcine Sapeloirus (Enterovirus A) SOP-BPA-0050 Porcine Teschovirus

Immunoperoxidase Test (IPT) / Peroxidase Linked Assay (PLA)

Malignant Catarrhal Fever SOP-BPA-0020

Polymerase Chain Reaction (PCR) Avian Influenza (rRT-PCR) SOP-AV-0068, WI-AV-0039 Newcastle Disease (rRT-PCR) SOP-AV-0068, WI-AV-0038 Avian Influenza (Additional Platforms) WI-AV-0035, WI-AV-0036, WI-AV-0037, WI-AV-0050

Swine Influenza Virus (rRT-PCR) SOP-BPA-0018 Malignant Catarrhal Fever (PCR) SOP-BPA-0015 Bovine Viral Diarrhea (PCR) SOP-BPA-0016 Porcine Teschovirus (PCR) SOP-BPA-0017 Bluetongue (RT-PCR) SOP-EO-0004 Epizootic Hemorrhagic Disease (RT-PCR) African Horse Sickness (RT-PCR) SOP-EO-0006 Eastern Equine Encephalomyelitis (RT-PCR) SOP-EO-0007 Western Equine Encephalomyelitis (RT-PCR) West Nile Virus (RT-PCR) Equine Influenza (rRT-PCR) SOP-AV-0068, WI-EO-0030

Virus Neutralization (VN)

Vesicular Stomatitis Virus (New Jersey andIndiana Strains) SOP-BPA-0013 Bovine Herpesvirus 1 (Infectious Bovine Rhintracheitis) SOP-BPA-0032 Bovine Herpesvirus 2 (Bovine Herpes Mammillitis) Bovine Respiratory Syncytial Virus Bovine Viral Diarrhea Parainfluenza-3 Encephalomyocarditis Virus SOP-BPA-0033 Malignant Catarrhal Fever SOP-BPA-0034 Pseudorabies Virus SOP-BPA-0035, SOP-BPA-0039 Transmissible Gastroenteritis Virus SOP-BPA-0036 Equine Herpesvirus 1 SOP-EO-0028 Equine Herpesvirus 4 Equine Viral Arteritis SOP-EO-0030 Bluetongue SOP-EO-0036 Epizootic Hemorrhagic Disease

Plaque Reduction Neutralization Test (PRNT)

West Nile Virus SOP-EO-0021 Eastern Equine Encephalomyelitis SOP-EO-0022

Western Equine Encephalomyelitis Venezuelan Equine Encephalomyelitis

Hemagglutination/Hemagglutination-Inhibition (HA/HI)

Avian Influenza (Virus Typing) SOP-AV-0009 Newcastle Disease (Virus Typing) SOP-AV-0010 Newcastle Disease (Serology) SOP-AV-0033 Avian Influenza (Serology) SOP-AV-0036 Swine Influenza Virus SOP-BPA-0027 Hemagglutinating Encephalomyelitis Virus SOP-BPA-0028 Eastern Equine Encephalomyelitis SOP-EO- 0023 Western Equine Encephalomyelitis Venezuelan Equine Encephalomyelitis Equine Influenza SOP-EO-0024

Neuraminidase-Inhibition (NI)

Avian Influenza (Virus Typing) SOP-AV-0011 Avian Influenza (Serology) SOP-AV-0012

Complement Fixation (CF)

Vesicular Stomatitis SOP-BPA-0010 Coxiella burnetti (Q-Fever) SOP-EO-0018 Chlamydophilia psittici SOP-EO-0035

Agar Gel Immunodiffusion (AGID)

Avian Influenza SOP-AV-0045 Equine Infectious Anemia SOP-EO-0034 Bluetongue (VDT) Commercial Kit SOP-EO-0017, REF-EO-0014 Bluetongue (VMRD) Commercial Kit SOP-EO-0017, REF-EO-0015 Bovine Leukosis Virus (VDT) Commercial Kit REF-EO- 0012 Epizootic Hemorrhagic Disease (VDT) Commercial Kit REF-EO- 0022 Caprine Arthritis-Encephalitis Virus (VDT) Commercial Kit REF-EO- 0023 Ovine Progressive Pneumonia Virus (VDT) Commercial Kit REF-EO-

Enzyme Linked Immunosorbent Assay (ELISA)

Avian Metapneumovirus SOP-AV-0024 Vesicular Stomatitis (C-ELISA) SOP-BPA- 0025 Porcine Reproductive and Respiratory Syndrome (IDEXX) Commercial Kit REF-BPA-0103 Pseudorabies Virus (gI ELISA) Commercial Kit SOP-BPA-0039, REF-BPA-0101 Pseudorabies Virus (gB ELISA) Commercial Kit SOP-BPA-0039, REF-BPA-0100 West Nile Virus (IgM ELISA) SOP-EO-0025 Eastern Equine Encephalomyelitis (IgM ELISA) SOP-EO-0026 Venezuelan Equine Encephalomyelitis (IgM ELISA) SOP-EO-0027 Equine Infectious Anemia (IDEXX, C-ELISA)Commercial Kit SOP-EO-0034, REF-EO- 0002 Equine Infectious Anemia (Synbiotics, ViraCHEK) Commercial Kit SOP-EO-0034, REF-EO- 0003 Equine Infectious Anemia (Centaur, Fusion Protein II) Commercial Kit SOP-EO-0034, REF-EO- 0004 Equine Infectious Anemia (VMRD) Commercial Kit SOP-EO-0034, REF-EO- Bluetongue (VDT) Commercial Kit REF-EO-0016 Bluetongue (VMRD) Commercial Kit REF-EO-0018

Bovine Leukosis Virus (VMRD) Commercial Kit REF-EO- 0010 Caprine Arthritis-Encephalitis Virus (VMRD) Commercial Kit REF-EO- 0020 Coxiella burnetti (Q-Fever) (IDEXX)Commercial Kit REF-EO-

Pseudorabies Virus (Autolex) Commercial Kit SOP-BPA-0039, REF-BPA-0102

In Vivo

Intracerebral Pathogenicity Index SOP-AV-0017 Intravenous Pathogenicity Index Chicken Lethality Test SOP-AV-0014

SCOPE OF ACCREDITATION TO ISO/IEC 17025:2017

USDA-APHIS-VS-NVSL

DIAGNOSTIC BACTERIOLOGY AND PATHOLOGY LABORATORY

DIAGNOSTIC BIOANALYTICAL AND REAGENT LABORATORY

1920 Dayton Avenue

CHEMICAL - 2

Test Type/Platform Test Methods

Chemistry and Analytical Services

HPLC

Coumaphos/Potasan SOP-CAS-0005 Ivermectin SOP-CAS-0003

Colorimetric

Total Protein/Cholesterol SOP-CAS-0117 pH SOP-CAS-0001

NATIONAL CENTER FOR ANIMAL HEALTH CALIBRATION LABORATORY

1920 Dayton Avenue Ames, Iowa 50010

CALIBRATION - 3

I. Chemical

Parameter/Equipment

Range CMC2 (±)

Comments

Gas Detection Monitors – CO2 (Carbon Dioxide)

5 % CO2

1.1 % CO2

Certified CO2

II. Mechanical

Mass – Weights and Weight Sets

1 mg 2 mg 3 mg 5 mg 10 mg 20 mg 30 mg 50 mg 100 mg 200 mg 300 mg 500 mg 1 g

12 µg 13 µg 13 µg 12 µg 12 µg 17 µg 17 µg 13 µg 12 µg 20 µg 20 µg 13 µg 36 µg

Balances using ASTM Class 1 weights

Mass – Weights and Weight Sets (cont)

2 g 3 g 5 g 10 g 20 g 30 g 50 g 100g (200 to 500) g 1000 g 2000 g 3000 g 5000 g

36 µg 38 µg 38 µg 86 µg

0.12 mg

0.14 mg

0.16 mg

0.27 mg

0.54 mg

0.60 mg

0.99 mg

0.67 mg

0.87 mg

Balances using ASTM Class 1 weights

Balances

0.01 Resolution:

(0 to 5) mg (5.1 mg to 1 g (1.1 to 2) g (2.1 to 3) g (3.1 to 5) g

0.01 mg Resolution:

(0 to 5) mg

5.1 mg to 10 g (10.1 to 30) g (30.1 to 40) g (40.1 to 70) g (70.1 to 150) g (150.1 to 250) g (250.1 to 350) g (350.1 to 400) g (400.1 to 500) g (500.1 to 600) g

0.1 mg Resolution:

(0 to 30) g

30.1 mg to 40 g (40.1 to 70) g (70.1 to 150) g (150.1 to 200) g (200.1 to 350) g (350.1 to 400) g (400.1 to 500) g (501 to 600) g (601 to 700) g (701 to 1000) g

0.0037 mg

0.0056 mg

0.014 mg

0.018 mg

0.039 mg

0.013 mg

0.20 mg

0.21 mg

0.049 mg

0.091 mg

0.11 mg

0.19 mg

0.20 mg

0.21 mg

0.30 mg

0.31 mg

0.12 mg

0.13 mg

0.15 mg

0.16 mg

0.22 mg

0.23 mg

0.24 mg

0.32 mg

0.33 mg

0.36 mg

0.94 mg

Balances (cont)

0.1 mg Resolution:

(1001 to 1500) g (1501 to 3000) g (3001 to 10 000) g

0.001 g Resolution:

(0 to 600) g (600.1 to 700) g (700.1 to 1500) g (1501 to 3000) g (3001 to 8000) g (8001 to 10 000) g

0.01 g Resolution:

(0 to 3000) g (3001 to 10 000) g

0.1 g Resolution:

(0 to 10) kg

0.95 mg

2.9 mg

3.4 mg

1.2 mg

1.3 mg

1.5 mg

3.1 mg

3.6 mg

3.8 mg

12 mg 13 mg

120 mg

Pipettes (0.4 to 2.5) µL

(2.5 to 20) µL (21 to 100) µL (101 to 200) µL (201 to 1000) µL (1001 to 5000) µL (5001 to 10 000) µL

16 nL 81 nL 66 nL 380 nL

2.3 µL 21 µL 120 µL

Gravimetric calibration using balances with

Centrifuge – Rotation (0 to 1000) rpm

(1001 to 6600) rpm (6601 to 20 000) rpm

13 rpm 120 rpm 130 rpm

Tachometer

III. Thermodynamics

Range CMC2, 4 (±)

Digital and Mechanical Thermometry Systems

≥ 0.01 ºC Resolution (-40 to 149) ºC

0.1 ºC Resolution

1 ºC Resolution

≥ 0.01 ºC Resolution (-94 to 139) ºC

0.1 ºC Resolution

0.043 ºC

0.065 ºC

0.61 ºC

0.19 ºC

0.20 ºC

0.61 ºC

PRT and reader and Hart Scientific / Fluke 7341 or 7103 calibration bath

PRT and reader and Hart Scientific / Fluke 9109A, 9100S, or 9102S heat dwell

Temperature – Measure and Measuring Equipment

(-196 to 250) ºC

≥ 0.1 ºC Resolution

2.4 ºC

0.28 ºC

0.65 ºC

Thermocouple thermometer and T-type or K-type thermocouples

PRT and reader

Autoclaves – Temperature Uniformity and Temperature Accuracy

(100 to 140) ºC

0.5 ºC

Data loggers

Commercial calibration service is sometimes available for this laboratory. This laboratory typically provides calibration service for the National Veterinary Services Laboratory and the Center for Veterinary Biologics Laboratory.

Calibration and Measurement Capability Uncertainty (CMC) is the smallest uncertainty of measurement that a laboratory can achieve within its scope of accreditation when performing more or less routine calibrations of nearly ideal measurement standards or nearly ideal measuring equipment. CMCs represent expanded uncertainties expressed at approximately the 95 % level of confidence, usually using a coverage factor of k = 2. The actual measurement uncertainty of a specific calibration performed by the laboratory may be greater than the CMC due to the behavior of the customer’s device and to influences from the circumstances of the specific calibration.

USDA APHIS VS NVSL

PLUM ISLAND ANIMAL DISEASE CENTER

NATIONAL VETERINARY SERVICES LABORATORIES

FOREIGN ANIMAL DISEASE DIAGNOSTIC LABORATORY

P.O. Box 848 Greenport, NY 11944

BIOLOGICAL - 4

Test Type/Platform Test Method(s) Agar Gel Immunodiffusion (AGID)

Foot-and-Mouth Disease SOP-DS-0008 Avidin Biotin Complex Assay

Classical Swine Fever SOP-DS-0035 Complement Fixation Test

Vesicular Stomatitis SOP-DS-0011 Direct Fluorescent Antibody

African Swine Fever SOP-DS-0054 DNA and RNA Extraction SOP DS-0077 Electron Microscopy SOP-DS-0053

Enzyme Linked Immunosorbent Assay (ELISA)

African Swine Fever SOP-DS-0013 Classical Swine Fever SOP-DS-0020 Foot-and-Mouth Disease SOP-DS-0004, SOP-DS-0007 Rabbit Hemorrhagic Disease SOP-DS-0063, SOP-DS-0064 Swine Vesicular Disease SOP-DS-0004 Vesicular Stomatitis SOP-DS-0004, SOP-DS-0010

Immunohistochemistry (IHC) African Swine Fever SOP-DS-0065 Classical Swine Fever SOP-DS-0065

Immunoperoxidase (IP) African Swine Fever SOP-DS-0083 Classical Swine Fever SOP-DS-0037

Real-time PCR African Swine Fever SOP DS-0081 Classical Swine Fever SOP DS-0081 Foot-and-Mouth Disease SOP DS-0081 Parapox SOP DS-0081 Swine Vesicular Disease SOP DS-0081

Virus Isolation (VI) African Swine Fever SOP-DS-0045 Classical Swine Fever SOP-DS-0021 Bovine Papular Stomatitis SOP-DS-0033 Contagious Ecthyma SOP-DS-0033 Foot-and-Mouth Disease SOP-DS-0033

Swine Vesicular Disease SOP-DS-0033 Vesicular Exanthema of Swine SOP-DS-0033 Vesicular Stomatitis SOP-DS-0033

Virus Neutralization (VN) Foot-and-Mouth Disease SOP-DS-0006 Swine Vesicular Disease SOP-DS-0014

SCOPE OF ACCREDITATION TO ISO/IEC 17034:2016

NATIONAL VETERINARY SERVICES

LABORATORIES USDA-APHIS-VS-NVSL

DIAGNOSTIC BACTERIOLOGY AND PATHOLOGY

LABORATORY DIAGNOSTIC BIOANALYTICAL AND REAGENT

LABORATORY

1920 Dayton Avenue

Reference Material Producer Scope – 5

Type of Reference Material Test and Matrix Measurement Method

Virology Reference Antiserum – Avian Influenza (AI) Virus

Identify Avian Influenza antibody

Positive Control Sera Strong Positive Reference Sera Weak Positive Reference Sera Negative Reference Sera

Virology Reference Antigen – Avian Influenza (AI) Virus

Identify Avian Influenza antibody

Antigen

Brucellosis Agglutination Test Antigen – Brucella abortus

Screening or Confirmatory Test for Brucella spp. antibody

Card Test Rivanol Plate Test RAP Test Buffered Acidified Plate Antigen Plate Agglutination Test Tube Agglutination Test

Brucellosis Control Serum – Brucella abortus

Positive Control Sera

Negative Control Sera

Card Test Rivanol Plate Test RAP Test Buffered Acidified Plate Antigen Plate Agglutination Test Tube Agglutination Test Complement Fixation

FPA

Salmonella Microtiter Test Antigen Salmonella pullorum

Antigen

Microtiter Agglutination Test

SCOPE OF ACCREDITATION TO ISO/IEC 17043:2010

1920 Dayton Avenue

PROFICIENCY TESTING PROVIDER - 6

PT Panel

PT Type

PT Plan/Method

Technique Used to Determine Assigned Values/Uncertainties

Diagnostic Bacteriology and Pathology Laboratory – Bacterial Identification

Contagious Equine Metritis -Taylorella equigenitalis

Isolation from Culture

SOP-BI-0114

Known Values

Group D Salmonella

MALDI

Isolation

SOP-BI-0124

Leptospirosis -Serovar Canicola -Serovar Grippo -Serovar Hardjo -Serovar Copenhageni (Icterohaemorrhagiae) -Serovar Pomona

Microscopic Agglutination Test

(MAT)

SOP-BI-0112

SOP-BI-0111

Consensus Values from Participants

Diagnostic Bioanalytical and Reagent Laboratory – BMRT/HRU

Bovine Tuberculosis -Mycobacterium bovis

Bovigam™ Gamma- Interferon

SOP-BMRT-0041

Diagnostic Bioanalytical and Reagent Laboratory – Serology

Equine Piroplasmosis -Babesia caballi -Theileria equi cELISA

WI-SERO-0068

Diagnostic Virology Laboratory – Avian

Avian Influenza Swine Influenza Avian Paramyxovirus type-1 rRT-PCR

REF-AV-0001

SCOPE OF ACCREDITATION TO ISO/IEC 17043:2010

USDA APHIS VS NVSL

PLUM ISLAND ANIMAL DISEASE CENTER

NATIONAL VETERINARY SERVICES

LABORATORIES

FOREIGN ANIMAL DISEASE DIAGNOSTIC

LABORATORY

P.O. Box 848

Greenport, NY 11944

PROFICIENCY TESTING PROVIDER - 7

Test Panel Method Technique Used to Determine Assigned Values / Uncertainties

Foot and Mouth Disease rRT-PCR Consensus Values from Expert Participants

Classical Swine Fever rRT-PCR Consensus Values from Expert Participants