Attachment_B-_WCIA_Analysis_Reports.pdf
PDF 356 KB Posted
- Attached to
- Surplus Seed Sale - Cream Gentian
- Solicitation number
- 2026-RFB-043-PR
- Issued by
- Dane County, Mattoon Village, Wisconsin
About this file
This document comprises seed analysis reports issued by the Wisconsin Crop Improvement Association (WCIA) laboratory in Middleton, Wisconsin, for cream gentian (Gentiana flavida) seed samples submitted by Wayne Pauly of Dane County Parks. Eight seed lots (numbered 1 through 8) were analyzed between February 5, 2026 (entry date) and March 30, 2026 (final completion date), with all results reported by April 1, 2026. Each sample underwent purity testing, warm germination testing, and noxious weed seed examination in accordance with AOSA rules. The seed lots were classified as "Service Only" status with no class specifically requested, and all testing was conducted at the USDA-accredited laboratory facility.
The analysis results demonstrate overall high seed quality across most lots, with pure seed percentages ranging from 61.46% to 71.62% and germination rates between 89% and 97%. Lot 3 exhibited the lowest purity at 61.46% with the highest inert matter content at 38.54%, while Lot 4 achieved the highest purity at 70.26% with excellent germination at 97%. Lot 6 contained one prohibited amaranth seed (Amaranthus spp.) per pound, the only noxious weed seed detected across all samples. Lots 1, 3, 4, 5, and 8 were free of noxious weeds and other crop contaminants. Lots 7, 8, and others contained trace other crop seeds including St. John's Wort, Blue Vervain, Yellow Coneflower, Hoary Vervain, Great Blue Lobelia, and Tall Cinquefoil. No hard seed dormancy was recorded across any lots, with dormancy determination performed using tetrazolium (TZ) testing. All analysis results are held confidential per USDA, OECD, and DATCP regulations.
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WISCONSIN CROP IMPROVEMENT ASSOCIATION
8520 UNIVERSITY GREEN • MIDDLETON, WI 53562
REPORT OF SEED ANALYSIS
PHONE: 608-262-0167
800-892-1341
FAX: 608-262-0210
EMAIL: wcia@mailplus.wisc.edu
Sample: 93044 Account: 5579
Entry date: 2/5/2026 Germination: 3/3/2026 Completed: 4/1/2026 Reported: 4/1/2026
Wayne Pauly Dane County Parks 4318 Robertson Rd Madison, WI 53714
Sender's Information—provided by sender and not laboratory
Variety: Variety Not Stated Kind: Cream Gentian (Gentiana flavida) Production Year:
Field Number: n/a Producer Lot Num: 1 Class Requested: Service Only Origin: n/a
Status: Service Only
Completed Tests: Purity, Warm Germination, Noxious
Pure Seed
Warm Germination
PLS
Germ
Cream Gentian (Gentiana flavida)† 67.89 0 x 94§ 94 21 400 20-30° 63.82
Total Pure Seed 67.89 0.3912 grams analyzed
Other Crop Seed 0.26 Noxious Exam Weights (g): Weight Analyzed: 2.6000 Inert Matter 31.85
Weed Seed 0.00
Noxious Weed Seeds in 2.6000 g # Found Per Lb
None found
Totals 0 0
Other Crop Seeds in 0.3912 g # Found Per Lb
Hoary Vervain (Verbena stricta) 1 1,159
Great Blue Lobelia (Lobelia siphilitica) 1 1,159
Tall Cinquefoil (Drymocallis arguta) 1 1,159
Totals 3 3,477
Weed Seeds in 0.3912 g # Found Per Lb
Inert Matter in 0.3912 g
Chaff
Additional Comments † AOSA has no defined rules for this test and species.
§ Dormancy was determined using TZ testing.
Shaminder Miranpuri, General Manager
RST Seal No. 26 RGT Seal No. 1016
All documentation and information generated by the services of the Association shall be kept confidential between the Association and its individual members/customers, except as necessary under USDA, OECD and DATCP agencies' regulations. The purity, noxious and germination tests reported on this form have been carried out in accordance with AOSA rules unless otherwise specified.
Test results reflect the condition of the submitted seed sample.
‡WCIA is a USDA Accredited Seed Laboratory approved for Seed Germination and Purity for Cereals and other Crops; Grasses; Legumes; Vegetable, Flowers and Herbs.
Purity
Hard
Dormant
Total Viable %
Days Tested
Seeds Temp mailto:wcia@mailplus.wisc.edu
REPORT OF SEED ANALYSIS
PHONE: 608-262-0167
800-892-1341
FAX: 608-262-0210
EMAIL: wcia@mailplus.wisc.edu
Sample: 93045 Account: 5579
Entry date: 2/5/2026 Germination: 3/3/2026 Completed: 4/1/2026 Reported: 4/1/2026
Wayne Pauly Dane County Parks 4318 Robertson Rd Madison, WI 53714
Sender's Information—provided by sender and not laboratory
Variety: Variety Not Stated Kind: Cream Gentian (Gentiana flavida) Production Year:
Field Number: n/a Producer Lot Num: 2 Class Requested: Service Only Origin: n/a
Status: Service Only
Completed Tests: Purity, Warm Germination, Noxious
Pure Seed
Warm Germination
PLS
Germ
Cream Gentian (Gentiana flavida)† 68.15 0 x 91§ 91 21 400 20-30° 62.02
Total Pure Seed 68.15 0.3856 grams analyzed
Other Crop Seed 0.00 Noxious Exam Weights (g): Weight Analyzed: 2.6000 Inert Matter 31.85
Weed Seed 0.00
Noxious Weed Seeds in 2.6000 g # Found Per Lb
Other Crop Seeds in 0.3856 g # Found Per Lb
Totals 0 0
Weed Seeds in 0.3856 g # Found Per Lb
Inert Matter in 0.3856 g
Additional Comments † AOSA has no defined rules for this test and species.
§ Dormancy was determined using TZ testing.
Shaminder Miranpuri, General Manager
RST Seal No. 26 RGT Seal No. 1016
All documentation and information generated by the services of the Association shall be kept confidential between the Association and its individual members/customers, except as necessary under USDA, OECD and DATCP agencies' regulations. The purity, noxious and germination tests reported on this form have been carried out in accordance with AOSA rules unless otherwise specified.
Test results reflect the condition of the submitted seed sample.
‡WCIA is a USDA Accredited Seed Laboratory approved for Seed Germination and Purity for Cereals and other Crops; Grasses; Legumes; Vegetable, Flowers and Herbs.
Purity
Hard
Dormant
Total Viable %
Days Tested
REPORT OF SEED ANALYSIS
PHONE: 608-262-0167
800-892-1341
FAX: 608-262-0210
EMAIL: wcia@mailplus.wisc.edu
Sample: 93046 Account: 5579
Entry date: 2/5/2026 Germination: 3/3/2026 Completed: 3/26/2026 Reported: 3/26/2026
Wayne Pauly Dane County Parks 4318 Robertson Rd Madison, WI 53714
Sender's Information—provided by sender and not laboratory
Variety: Variety Not Stated Kind: Cream Gentian (Gentiana flavida) Production Year:
Field Number: n/a Producer Lot Num: 3 Class Requested: Service Only Origin: n/a
Status: Service Only
Completed Tests: Purity, Warm Germination, Noxious
Pure Seed
Warm Germination
PLS
Germ
Cream Gentian (Gentiana flavida)† 61.46 0 x 95§ 95 21 400 20-30° 58.39
Total Pure Seed 61.46 0.2592 grams analyzed
Other Crop Seed 0.00 Noxious Exam Weights (g): Weight Analyzed: 2.6000 Inert Matter 38.54
Weed Seed 0.00
Noxious Weed Seeds in 2.6000 g # Found Per Lb
Other Crop Seeds in 0.2592 g # Found Per Lb
Totals 0 0
Weed Seeds in 0.2592 g # Found Per Lb
Inert Matter in 0.2592 g
Broken Seed
Additional Comments † AOSA has no defined rules for this test and species.
§ Dormancy was determined using TZ testing.
Shaminder Miranpuri, General Manager
RST Seal No. 26 RGT Seal No. 1016
All documentation and information generated by the services of the Association shall be kept confidential between the Association and its individual members/customers, except as necessary under USDA, OECD and DATCP agencies' regulations. The purity, noxious and germination tests reported on this form have been carried out in accordance with AOSA rules unless otherwise specified.
Test results reflect the condition of the submitted seed sample.
‡WCIA is a USDA Accredited Seed Laboratory approved for Seed Germination and Purity for Cereals and other Crops; Grasses; Legumes; Vegetable, Flowers and Herbs.
Purity
Hard
Dormant
Total Viable %
Days Tested
REPORT OF SEED ANALYSIS
PHONE: 608-262-0167
800-892-1341
FAX: 608-262-0210
EMAIL: wcia@mailplus.wisc.edu
Sample: 93047 Account: 5579
Entry date: 2/5/2026 Germination: 3/3/2026 Completed: 3/27/2026 Reported: 3/27/2026
Wayne Pauly Dane County Parks 4318 Robertson Rd Madison, WI 53714
Sender's Information—provided by sender and not laboratory
Variety: Variety Not Stated Kind: Cream Gentian (Gentiana flavida) Production Year:
Field Number: n/a Producer Lot Num: 4 Class Requested: Service Only Origin: n/a
Status: Service Only
Completed Tests: Purity, Warm Germination, Noxious
Pure Seed
Warm Germination
PLS
Germ
Cream Gentian (Gentiana flavida)† 70.26 0 x 97§ 97 21 400 20-30° 68.15
Total Pure Seed 70.26 0.3756 grams analyzed
Other Crop Seed 0.00 Noxious Exam Weights (g): Weight Analyzed: 2.6000 Inert Matter 29.74
Weed Seed 0.00
Noxious Weed Seeds in 2.6000 g # Found Per Lb
Other Crop Seeds in 0.3756 g # Found Per Lb
Totals 0 0
Weed Seeds in 0.3756 g # Found Per Lb
Inert Matter in 0.3756 g
Additional Comments † AOSA has no defined rules for this test and species.
§ Dormancy was determined using TZ testing.
Shaminder Miranpuri, General Manager
RST Seal No. 26 RGT Seal No. 1016
All documentation and information generated by the services of the Association shall be kept confidential between the Association and its individual members/customers, except as necessary under USDA, OECD and DATCP agencies' regulations. The purity, noxious and germination tests reported on this form have been carried out in accordance with AOSA rules unless otherwise specified.
Test results reflect the condition of the submitted seed sample.
‡WCIA is a USDA Accredited Seed Laboratory approved for Seed Germination and Purity for Cereals and other Crops; Grasses; Legumes; Vegetable, Flowers and Herbs.
Purity
Hard
Dormant
Total Viable %
Days Tested
REPORT OF SEED ANALYSIS
PHONE: 608-262-0167
800-892-1341
FAX: 608-262-0210
EMAIL: wcia@mailplus.wisc.edu
Sample: 93048 Account: 5579
Entry date: 2/5/2026 Germination: 3/3/2026 Completed: 3/27/2026 Reported: 3/27/2026
Wayne Pauly Dane County Parks 4318 Robertson Rd Madison, WI 53714
Sender's Information—provided by sender and not laboratory
Variety: Variety Not Stated Kind: Cream Gentian (Gentiana flavida) Production Year:
Field Number: n/a Producer Lot Num: 5 Class Requested: Service Only Origin: n/a
Status: Service Only
Completed Tests: Purity, Warm Germination, Noxious
Pure Seed
Warm Germination
PLS
Germ
Cream Gentian (Gentiana flavida)† 68.19 0 x 89§ 89 21 400 20-30° 60.69
Total Pure Seed 68.19 0.3942 grams analyzed
Other Crop Seed 0.00 Noxious Exam Weights (g): Weight Analyzed: 2.6000 Inert Matter 31.81
Weed Seed 0.00
Noxious Weed Seeds in 2.6000 g # Found Per Lb
Other Crop Seeds in 0.3942 g # Found Per Lb
Totals 0 0
Weed Seeds in 0.3942 g # Found Per Lb
Inert Matter in 0.3942 g
Additional Comments † AOSA has no defined rules for this test and species.
§ Dormancy was determined using TZ testing.
Shaminder Miranpuri, General Manager
RST Seal No. 26 RGT Seal No. 1016
All documentation and information generated by the services of the Association shall be kept confidential between the Association and its individual members/customers, except as necessary under USDA, OECD and DATCP agencies' regulations. The purity, noxious and germination tests reported on this form have been carried out in accordance with AOSA rules unless otherwise specified.
Test results reflect the condition of the submitted seed sample.
‡WCIA is a USDA Accredited Seed Laboratory approved for Seed Germination and Purity for Cereals and other Crops; Grasses; Legumes; Vegetable, Flowers and Herbs.
Purity
Hard
Dormant
Total Viable %
Days Tested
REPORT OF SEED ANALYSIS
PHONE: 608-262-0167
800-892-1341
FAX: 608-262-0210
EMAIL: wcia@mailplus.wisc.edu
Sample: 93049 Account: 5579
Entry date: 2/5/2026 Germination: 3/3/2026 Completed: 3/27/2026 Reported: 3/27/2026
Wayne Pauly Dane County Parks 4318 Robertson Rd Madison, WI 53714
Sender's Information—provided by sender and not laboratory
Variety: Variety Not Stated Kind: Cream Gentian (Gentiana flavida) Production Year:
Field Number: n/a Producer Lot Num: 6 Class Requested: Service Only Origin: n/a
Status: Service Only
Completed Tests: Purity, Warm Germination, Noxious
Pure Seed
Warm Germination
PLS
Germ
Cream Gentian (Gentiana flavida)† 71.37 0 x 92§ 92 21 400 20-30° 65.66
Total Pure Seed 71.37 0.3675 grams analyzed
Other Crop Seed 0.00 Noxious Exam Weights (g): Weight Analyzed: 2.600 Inert Matter 28.41
Weed Seed 0.22
Noxious Weed Seeds in 2.600 g # Found Per Lb
Amaranth (Amaranthus spp.), Prohibited [GA & IN] 1 174
Totals 1 175
Other Crop Seeds in 0.3675 g # Found Per Lb
Totals 0 0
Weed Seeds in 0.3675 g # Found Per Lb
1 1,234
Totals 1 1,234
Inert Matter in 0.3675 g
Additional Comments † AOSA has no defined rules for this test and species.
§ Dormancy was determined using TZ testing.
Shaminder Miranpuri, General Manager
RST Seal No. 26 RGT Seal No. 1016
All documentation and information generated by the services of the Association shall be kept confidential between the Association and its individual members/customers, except as necessary under USDA, OECD and DATCP agencies' regulations. The purity, noxious and germination tests reported on this form have been carried out in accordance with AOSA rules unless otherwise specified.
Test results reflect the condition of the submitted seed sample.
‡WCIA is a USDA Accredited Seed Laboratory approved for Seed Germination and Purity for Cereals and other Crops; Grasses; Legumes; Vegetable, Flowers and Herbs.
Purity
Hard
Dormant
Total Viable %
Days Tested
REPORT OF SEED ANALYSIS
PHONE: 608-262-0167
800-892-1341
FAX: 608-262-0210
EMAIL: wcia@mailplus.wisc.edu
Sample: 93050 Account: 5579
Entry date: 2/5/2026 Germination: 3/3/2026 Completed: 3/30/2026 Reported: 3/30/2026
Wayne Pauly Dane County Parks 4318 Robertson Rd Madison, WI 53714
Sender's Information—provided by sender and not laboratory
Variety: Variety Not Stated Kind: Cream Gentian (Gentiana flavida) Production Year:
Field Number: n/a Producer Lot Num: 7 Class Requested: Service Only Origin: n/a
Status: Service Only
Completed Tests: Purity, Warm Germination, Noxious
Pure Seed
Warm Germination
PLS
Germ
Cream Gentian (Gentiana flavida)† 71.62 0 x 95§ 95 21 400 20-30° 68.04
Total Pure Seed 71.62 0.3683 grams analyzed
Other Crop Seed 0.22 Noxious Exam Weights (g): Weight Analyzed: 2.6000 Inert Matter 28.16
Weed Seed 0.00
Noxious Weed Seeds in 2.6000 g # Found Per Lb
Other Crop Seeds in 0.3683 g # Found Per Lb
St John's Wort (Hypericum perforatum) 2 2,463
Totals 2 2,463
Weed Seeds in 0.3683 g # Found Per Lb
Inert Matter in 0.3683 g
Additional Comments † AOSA has no defined rules for this test and species.
§ Dormancy was determined using TZ testing.
Shaminder Miranpuri, General Manager
RST Seal No. 26 RGT Seal No. 1016
All documentation and information generated by the services of the Association shall be kept confidential between the Association and its individual members/customers, except as necessary under USDA, OECD and DATCP agencies' regulations. The purity, noxious and germination tests reported on this form have been carried out in accordance with AOSA rules unless otherwise specified.
Test results reflect the condition of the submitted seed sample.
‡WCIA is a USDA Accredited Seed Laboratory approved for Seed Germination and Purity for Cereals and other Crops; Grasses; Legumes; Vegetable, Flowers and Herbs.
Purity
Hard
Dormant
Total Viable %
Days Tested
REPORT OF SEED ANALYSIS
PHONE: 608-262-0167
800-892-1341
FAX: 608-262-0210
EMAIL: wcia@mailplus.wisc.edu
Sample: 93051 Account: 5579
Entry date: 2/5/2026 Germination: 3/3/2026 Completed: 3/30/2026 Reported: 3/30/2026
Wayne Pauly Dane County Parks 4318 Robertson Rd Madison, WI 53714
Sender's Information—provided by sender and not laboratory
Variety: Variety Not Stated Kind: Cream Gentian (Gentiana flavida) Production Year:
Field Number: n/a Producer Lot Num: 8 Class Requested: Service Only Origin: n/a
Status: Service Only
Completed Tests: Purity, Warm Germination, Noxious
Pure Seed
Warm Germination
PLS
Germ
Cream Gentian (Gentiana flavida)† 70.13 0 x 97§ 97 21 400 20-30° 68.03
Total Pure Seed 70.13 0.3754 grams analyzed
Other Crop Seed 0.19 Noxious Exam Weights (g): Weight Analyzed: 2.6000 Inert Matter 29.68
Weed Seed 0.00
Noxious Weed Seeds in 2.6000 g # Found Per Lb
Other Crop Seeds in 0.3754 g # Found Per Lb
Blue Vervain (Verbena hastata) 1 1,208
Yellow Coneflower (Ratibida pinnata) 1 1,208
Totals 2 2,416
Weed Seeds in 0.3754 g # Found Per Lb
Inert Matter in 0.3754 g
Additional Comments † AOSA has no defined rules for this test and species.
§ Dormancy was determined using TZ testing.
Shaminder Miranpuri, General Manager
RST Seal No. 26 RGT Seal No. 1016
All documentation and information generated by the services of the Association shall be kept confidential between the Association and its individual members/customers, except as necessary under USDA, OECD and DATCP agencies' regulations. The purity, noxious and germination tests reported on this form have been carried out in accordance with AOSA rules unless otherwise specified.
Test results reflect the condition of the submitted seed sample.
‡WCIA is a USDA Accredited Seed Laboratory approved for Seed Germination and Purity for Cereals and other Crops; Grasses; Legumes; Vegetable, Flowers and Herbs.
Purity
Hard
Dormant
Total Viable %
Days Tested
Seeds Temp mailto:wcia@mailplus.wisc.edu
| WCIAAnalysisReport 1 and 2.pdf |
| WCIAAnalysisReport 3.pdf |
| WCIAAnalysisReport 4.pdf |
| WCIAAnalysisReport 5 and 6.pdf |
| WCIAAnalysisReport 7 and 8.pdf |
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