Attachment 2 - Statement of Requirements.pdf
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- Solicitation number
- 1232SA26Q0960
About this file
This document is a technical specifications file for a federal procurement of laboratory imaging equipment and supplies by the Roman L. Hruska U.S. Meat Animal Research Center (USMARC), a USDA-ARS facility.
The primary deliverable is a blots and gels imaging system that must be compatible with Image Lab Software and capable of accessing and modifying ptl and scn file types. The imaging system must include a cooled CCD detector with 6 megapixels, dynamic range exceeding 4 orders of magnitude, and a chemiluminescence detector with 590/110 nm standard emission filter. Additional technical requirements include automatic tray recognition, pre-calibrated focus capabilities for any zoom setting, two user-defined auto-exposure modes, image flat fielding capability, 16-bit or 8-bit data output in SCN, TIFF, or JPEG formats, and physical dimensions not exceeding 61 cm length by 51 cm width by 53 cm height. The system must operate at 100-250V, with an operating temperature range of 10-28 degrees Celsius and humidity range of 10-85% relative humidity. Accompanying supplies required include one white sample tray, one blue sample tray (both for Coomassie Blue, Copper, Silver, or Zinc stains), Image Lab Software with FDA CFR 21 Part 11 module, hFAB Rhodamine Anti-Tubulin Primary Antibody (40 μl), Precision Plus Protein standards with three reference bands (25, 50, 75 kD) for 100 applications, Clarity Max Western ECL Substrate (100 ml), and a package of at least 10 4-20% Mini-Protean TGX Stain-Free Protein Gels with 12-month shelf life. All products must be delivered within 30 days of award to USMARC in Clay Center, Nebraska. The government is offering a Bio-Rad ChemiDoc XRS+ (Model Universal Hood II, Serial No. 721BR01140) as a trade-in, which will be exchanged upon delivery of the new equipment.
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Specifications Project: Blots and Gels Imaging System with Accompanying Supplies
1.0 Background and Purpose:
Scientists at the Roman L. Hruska U.S. Meat Animal Research Center (USMARC) are developing innovative technologies to address high-priority challenges within the U.S. beef, swine, and sheep industries. To support meat safety and quality research, a new blots and gels imaging system with accompanying supplies is required. This equipment is essential for the continuation of many research projects that require such technology and is a high priority item for USMARC.
CONTRACTOR REQUIREMENTS
2.0 Technical Requirements/Tasks:
• Blots and Gels Imaging System o Imaging System must be compatible with Image Lab Software o Capable to access and modify ptl and scn file types o Imaging system must have automatic recognition of application-specific tray and adjustment of imaging parameters and software options o Must have capabilities to preform pre-calibrated focus for any zoom setting or sample height o Must have two user-defined modes (rapid or optimal auto-exposure) for all blot and gel imaging applications o Must have capability of Image flat fielding o Must have Cooled CCD, 6 megapixels detector o Imaging system must have Dynamic Range of >4 orders magnitude o Imaging system must have a Chemiluminescence detector
590/110 nm standard emission filter for protein and DNA gel and blot imaging o Capable of data output, 16-bit or 8-bit: SCN, TIFF, JPEG files o Instrument size no larger the following to fit in the constraints of the laboratory Length, 61 cm Width, 51 cm Height, 53 cm o Operating Voltage must be 100-250 V to be compatible with electric of the building o Operating Temperature 10-28 degrees Celsius for research sample preservation o Operating Humidity 10-85% relative humidity for research sample preservation
• White Sample Tray o Quantity: 1 o Sample Tray for gels and blots stained with Coomassie Blue, Copper, Silver or Zinc Stains
• Blue Sample Tray o Quantity: 1 o Sample tray for gels and blots stained with Coomassie Blue, Copper, Silver or Zinc Stains
• Image Lab Software o Quantity: 1 o FDA CFR 21 Part 11 module for Image Lab Software
• hFAB Rhodamine Anti-Tubulin Primary Antibody 40 μl o Quantity: 1 o lyophilized rhodamine conjugated anti-tubulin human Fab fragment;
reconstitute to 40 μl
• Precision Plus Protein o Unstained protein standards, strep-tagged, recombinant proteins (10- 250kD) o Must include three reference bands (25,50, 75 kD) o 100 applications
• Clarity Max Western ECL Substrate o Quantity: 1 o 100 ml o Contain 50 ml clarity Max Western Peroxide Reagent o 50 ml Clarity Max Western Luminol/Enhancer Reagent
• 4-20% Mini-Protean TGX Stain-Free Protein Gels o Quantity: Package of at least 10 o 15 Well, 15 μl o 4--20% precast o polyacrylamide gel o 8.6 × 6.7 cm (W × L) o for use with Mini-o PROTEAN Electrophoresis Cells o 12-month shelf life
3.0 Government Furnished:
Trade- In: The USDA-ARS is offering as trade a Bio-Rad ChemiDoc XRS+ Model No: Universal Hood II Serial No: 721BR01140. Trades will not be released until the new Blots and Gels Imaging System is delivered. USDA will continue to use the existing equipment until the new unit is delivered. USDA has a loading doc and can assist with the unloading of new equipment and loading of trade in equipment.
4.0 Deliverables/ Schedules:
All products must be delivered within 30 days of award to:
USMARC
844 Road 313, Clay Center, NE 68933
Blots and Gels Imaging System
File details come from the government source that posted it. Updated .