Attachment 2 Specifications Solicitation 1232SA26Q0707.xlsx

This is a specifications document for a Request for Quotation (RFQ) issued by the Department of Agriculture Agricultural Research Service for the procurement of one Cytation 5 Cell Imaging Multi-Mode Reader. The instrument must meet specific technical requirements including capability for brightfield and fluorescence microscopy imaging of bacteria, incubation temperatures of 60°C or higher with shaking capability, monochromator-based fluorescence intensity measurement, and time-resolved fluorescence assays across wavelengths of 200-700 nm. Additional mandatory specifications include a minimum 6-log dynamic range for fluorescence measurement, user-changeable objectives and filters for imaging, and compatibility with microplates, slides, and dishes for tissue culture and bacterial applications. Optional but desirable features include gas control and reagent injector capabilities for future experimental expansion.

The procurement is justified as a sole source acquisition based on the agency's established protocol for time-lapse imaging of bacterial morphology using the Cytation 5 instrument, as documented in published research (Canadian Journal of Microbiology 66:303-312, 2020). The agency determined that only this specific instrument can simultaneously meet all stated requirements while providing flexibility for future addition of reagent injection and gas control systems. The solicitation is unrestricted and open to all responsible sources. The applicable NAICS code is 334516 with a small business size standard of 1,000 employees. This acquisition is not set-aside for small business concerns.