Attachment 2_Specifications_1232SA26Q1261.pdf

Specifications

Project Title: ZEISS Axioscope 5 upright compound microscope for transmitted and fluorescent light or equivalent

1. Objective and Scope

The USDA-ARS requires the procurement of a high-performance upright compound microscope system equipped for both transmitted and fluorescent light microscopy. The system must meet or exceed the exact technical specifications listed below to support advanced research applications.

All components must be fully integrated, compatible, and form a complete, operational imaging system.

A new high-performance upright compound microscope system equipped for both transmitted and fluorescent light microscopy is required for the USDA-ARS research laboratory in Wapato, WA. Currently, the laboratory relies on an aging microscopy stand that has reached the end of its operational lifespan. The image quality, optical clarity, and mechanical reliability of this existing system have fallen significantly below the technical requirements necessary to conduct precise, high-resolution cellular and structural analyses. To maintain research continuity and ensure data integrity, a complete replacement of this system is urgently needed. To optimize government spending, the laboratory intends to pour over and transfer several high-value components from the existing setup to the new unit. Specifically, the laboratory already possesses a specialized suite of fluorescence filters—including Green Fluorescent Protein (GFP), Cherry, DAPI, and specific low-pass filters—which remain in excellent working condition. Therefore, the requested microscope stand must feature a highly compatible, standard modular design (such as an encoded

6-position reflector turret) capable of seamlessly accepting and utilizing these existing filter cubes without requiring modification

2. Technical Specifications and Required Parts List

Vendors must provide a quote for a system that includes the following components meeting or exceeding these precise specifications:

Item

Technical Description Qty

Microscope Stand for Transmitted & Fluorescent Light (TL/FL)

• 6-position encoded brightfield/darkfield/differential interference contrast (H DIC) nosepiece, M27 thread size.

• Lower stand section with a dovetail illumination interface.

Item

Technical Description Qty

• Integrated transmitted-light illumination featuring a white 10W LED

(with options to accommodate 100W halogen).

• Dovetail mount for an attachable stage, a 24 mm focus lift with an adjustable focus stop, and an automatic mechanical safety shutter.

• Integrated 6-position filter wheel for 36 mm diameter filters and a mount for a 2-position filter slider (14x40 mm, d=36 mm).

• Controller Area Network (CAN) port for advanced LED/metal halide light source integration.

• Built-in 24V DC 60W stabilized power unit (100...240V AC, 50...60Hz) with a country-specific power cable.

• Eco-mode functionality and an integrated light management control button.

• Hardware snap-button support for dedicated microscope camera capture via USB 2.0 connection to a PC.

• Included dust cover and a transmitted-light filter holder.

• Upper stand part with a dovetail interface for reflected illumination

(supporting 100W halogen, mercury, metal halide, or multi-channel LED sources).

• Reflected-light illuminator featuring a white 10W LED.

• Dovetail mount for coded changeable reflector turrets and sliders.

• Designated mounts for: a luminous-field diaphragm slider, an aperture stop slider (or fluorescence attenuator), an HBO/XBO adjusting aid, and a

14x40 mm (d=36mm) reflector filter slider.

Binocular Ergonomic Phototube

• $20^{\circ}/23$ viewing angle with selectable light paths (100:0 / 0:100 split).

• Generates a reversed image.

• Features continuous vertical height adjustment of up to 44 mm.

• Includes a standardized 60N interface camera port.

Mechanical Stage

• Travel range of 75x50 mm (Right-hand drive) with a smooth, hardcoat anodized surface.

• Approximate stage plate dimensions of 220 x 150 mm.

• Rackless X-guide positioned at the bottom with a hidden gear mechanism in the Y-guide.

• Right-hand drive length of 135 mm (extendable by an additional 15

mm) equipped with adjustable friction settings.

Specimen Holder

Technical Description Qty

• Spring-lever (left-side operation) designed to securely accommodate up to two standard 76x26 mm glass slides simultaneously.

Stage Carrier

• Attachable, vertically adjustable stage carrier compatible with the requested microscope stand to accommodate screw-on mechanical stages.

Stop Slider (Aperture/Luminous Field)

• 14x40 mm stop slider featuring an integrated aperture and luminous-field diaphragm mechanism.

Stop Slider (Reflected-Light Luminous Field)

• 14x40 mm stop slider with a luminous-field diaphragm optimized for use with high-definition reflected-light/DIC illuminators.

Reflector Turret

• 6-position encoded, interchangeable turret designed for standard plug-and-play optical modules.

Condenser Carrier

• Heavy-duty carrier featuring dual-sided vertical height adjustment, compatible with attachable stage carriers, and including an adjustable height stop.

Achromatic-Aplanatic Condenser

• Numerical Aperture (NA) of 0.9 H, featuring an integrated front lens that can be manually switched from the right or left side.

Technical Description Qty

• Optimized for objective magnifications ranging from 1.0x to 100x, with a working distance (WD) of 1.0 mm.

High-Performance Objective - 1.25x

• Plan-Neofluar class (or equivalent high-contrast, color-corrected optical standard) 1.25x magnification.

• Numerical Aperture: 0.03.

• Thread type: M27.

• Free Working Distance (FWD): 4.0 mm.

High-Performance Objective - 5x

• Plan-Neofluar class 5x magnification.

• Numerical Aperture: 0.16.

• Free Working Distance (FWD): 18.5 mm.

High-Performance Objective - 10x

• Plan-Neofluar class 10x magnification.

• Numerical Aperture: 0.3.

• Free Working Distance (FWD): 5.2 mm.

High-Performance Objective - 20x

• Plan-Neofluar class 20x magnification.

• Numerical Aperture: 0.50.

• Free Working Distance (FWD): 2.0 mm.

High-Performance Objective - 40x

• Plan-Neofluar class 40x magnification.

• Numerical Aperture: 0.75.

• Free Working Distance (FWD): 0.71 mm.

• Designed for a standard cover glass thickness of 0.17 mm.

• Must include a matching kit of high-performance cover glasses

(minimum 100 pc).

High-Performance Oil Immersion Objective - 100x

• Plan-Neofluar class 100x oil immersion magnification.

• Numerical Aperture: 1.30.

• Free Working Distance (FWD): 0.20 mm.

• Designed for a standard cover glass thickness of 0.17 mm.

• Must include a compatible low-fluorescence immersion oil kit

(minimum 20ml oiler) and matching high-performance cover glasses

(minimum 100 pc).

Camera Adapter

• 60N-C mount camera adapter, 1" field of view, 1.0x magnification.

3. Special Requirements & Terms

• Shipping: Shipping and handling must be included at no additional cost to the government (FOB Destination).

• Delivery Location: USDA-ARS, 5230 Konnowac Pass Rd, Wapato, WA 98951.

• Component Integration: Quoted components must be completely compatible with standard microscopy modular components to allow seamless, stable assembly and long-term operation.

• PO Box 6755