Attachment 1 - Statement of Work.pdf

Statement of Work

Project: Soybean Elemental Analysis

Part I: General Information

A. Introduction –

We request elemental analysis of a diverse soybean population. Seeds were harvested from ambient and elevated carbon dioxide concentration in the Soybean Free Air CO2

Enrichment (SoyFACE) experiment, and we would like the concentrations of macro (N, P, K) and micronutrients (S, Ca, Mg, Fe, Mn, Ze, Cu, B, Al, Na) measured in the seeds.

B. Background –

GCPRU aims to improve the response of crops to global atmospheric change, including rising atmospheric carbon dioxide concentrations. Previous research has identified that rising atmospheric carbon dioxide concentration has a negative impact on seed quality, including micronutrient concentration. We have developed a recombinant inbred population to test how seed quality is related to yield response to elevated carbon dioxide in soybean. The proposed work would allow us to map quantitative trait loci associated with micronutrient content and response to elevated CO2 in soybean.

C. Scope -

The service that we request is analysis of the concentration of macro and micronutrients in soybean seeds. We have 705 samples that we would like to analyze and ~30 g of seed for each sample. We request measurement of N, P, K, S, Ca, Mg, Fe, Mn, Ze, Cu, B, Al, and Na.

Part II: Work Requirement

A. Technical Requirements –

We request analysis of N, P, K, S, Ca, Mg, Fe, Mn, Ze, Cu, B, Al, and Na content in soybean seeds. Analysis must be done on 30-40 g of seed and be done by equipment that is certified by the North American Proficiency Testing Program.

B. Deliverables

A report providing the results of elemental analysis for each of the 705 samples.

Part 3: Supporting Information

A. Place of Performance – Champaign, Illinois

B. Period of Performance – 31 March 2023 – 30 April 2023