Attachment 1 Statement of Work.pdf
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- Attached to
- Metagenomic Bioinformatics Analysis Federal contract opportunity
- Solicitation number
- 12905B25Q0022
About this file
This Statement of Work (SOW) outlines requirements for Metagenomic Bioinformatics Analysis services required by the USDA-ARS Salinity Lab in Riverside, CA. The contractor must analyze 1,275 soil and water DNA samples, consisting of 475 samples for Shotgun Metagenomics (WBI) and 800 samples for Amplicon Metagenomics Sequencing (WOBI).
The technical requirements include measuring DNA quantity and quality, ensuring samples pass quality control, constructing specialized DNA libraries, and performing DNA library sequencing using next-generation sequencing instruments. For deliverables, the contractor must generate 50-100k reads per sample for the 800 amplicon sequencing samples with standard data analysis, and 6Gb shotgun metagenomics with assembly-based analysis for the 475 DNA samples. All results must be uploaded to a hard disk and delivered to the USDA facility within 160 days after receipt of order (ARO). The government will furnish all 1,275 DNA samples for analysis.
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Other files for this federal contract opportunity
| File | Type | Posted |
|---|---|---|
| RFQ 12905B25Q022 Combined SynSOL Terms and Conditions.pdf | ||
| Attachment II Wage Determinations.pdf |
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Text version
Statement of Work (SOW) Metagenomic Bioinformatics Analysis
SERVICE
GENERAL INFORMATION
1.0 Scope of Work:
Contractor will provide Metagenomic Bioinformatics Analysis of 1275 soil and water DNA samples. The results are to be uploaded to a hard disk and sent to USDA-ARS Salinity Lab 450 W Big Springs Rd Riverside CA 92507
Samples will include:
1) 475 samples various microbial samples from water for Shotgun Metagenomics (WBI)
2) 800 samples various microbial samples from water for Amplicon Metagenomics Sequencing (WOBI)
2.0 Background:
USDA ARS Salinity Lab, Riverside CA has a need for Metagenomic Bioinformatics Analysis on Shotgun Metagenomics (WBI) and Amplicon Metagenomics Sequencing
(WOBI).
CONTRACTOR REQUIREMENTS
3.0 Technical Requirements/Tasks:
Service tasks include, but are not limited to:
1. Measure the DNA quantity and check the quality to determine whether the
DNA sample is good for library construction.
2. Ensure the samples pass quality control
3. Construct a specialized library using the DNA sample.
4. DNA library sequencing using a next-generation sequencing instrument.
4.0 Government Furnished:
1275 soil and water DNA samples
5.0 Deliverables / Schedule:
Item No Deliverable Objective Due 0001 Metagenomic
Bioinformatics Analysis
For each sample, the vendor will generate 50-100k reads per sample for amplicon sequencing of 800 DNA samples with standard data analysis and 6Gb shotgun metagenomics with assembly-based analysis of short sequence read of 475 DNA samples, and upload it into a hard disk, and ship to the customer.
160 Days ARO
6.0 Delivery Address:
450 W. Big Springs Rd., Riverside, CA 92507
16.0 Technical Point of Contract:
Dr. A. Mark Ibekwe mark.ibekwe@usda.gov 909-845-2809 mailto:mark.ibekwe@usda.gov
File details come from the government source that posted it. Updated .