Attachment_1_Statement_of_Work.pdf

PERFORMANCE-BASED WORK STATEMENT

I. GENERAL INFORMATION

A. Introduction: Work will involve chemical analyses of sediments to identify polycyclic aromatic hydrocarbons, total petroleum hydrocarbons, and trace metals in FY24 with an option for FY25

B. Background: The 2010 Deepwater Horizon (DWH) oil spill was an unprecedented event. Approximately 3.2 million barrels of oil were released into the deep ocean over nearly three months. The plume of oil moved throughout the water column, formed surface slicks that cumulatively covered an area the size of Virginia, and washed oil onto at least 1,300 miles of shoreline habitats. More than 770 square miles of deep benthic habitat surrounding the Deepwater Horizon wellhead and four-square miles of the Pinnacles mesophotic reef complex, located at the edge of the continental shelf, were injured by the oil spill.

As part of the deep benthic habitats, sediment environments were impacted by the DWH oil spill, with an estimated 4-31% of oil remaining trapped in the deep sea. The footprint of the DWH spill on soft-sediment communities in the norther Gulf of Mexico (GOM) estimated 263 km2 were affected by the spill, with macrofaunal abundance and diversity patterns driven by total hydrocarbons, PAHs, and barium concentrations. Severe impacts were found in an area of 58 km2 that also included reduced diversity and abundances of macrofauna. Similar to soft-sediment habitats, near-coral associated macrofauna communities were impacted, with high variance of density, diversity, and community structure associated with elevated hydrocarbon concentrations. Meiofaunal communities were also impacted by the DWH spill, with high densities and nematode: copepod ratios but low diversity a function of sediment TPH, PAH, and barium concentrations.

Recovery of deep sediment communities have been estimated to take decades or longer and documenting this recovery will be important for understanding the response of deep habitats to disturbance events like the DWH oil spill. Examining the sediment communities in soft-sediment and coral-associated habitats (work conducted at USGS WARC) combined with detailed geochemical analyses including hydrocarbons and trace metals (scope defined below) will allow for documentation of important baseline information to inform recovery trajectories. They will also improve our understanding of the spatial distribution of communities across the northern GOM and the influence of both natural and anthropogenic influences and changes.

C. Definitions/Applicable Documents:

Reuscher MG, Baguley JG, Montagna PA (2020) The expanded footprint of the Deepwater Horizon oil spill in the Gulf of Mexico deep-sea benthos. PLoS ONE 15(6): e0235167.

https://doi.org/10.1371/journal.pone.0235167

Demopoulos, A. W., Bourque, J. R., Cordes, E., & Stamler, K. M. (2016). Impacts of the

Deepwater Horizon oil spill on deep-sea coral-associated sediment communities. Marine Ecology Progress Series, 561, 51-68.

ANALYTICAL QUALITY ASSURANCE PLAN MISSISSIPPI CANYON 252 (DEEPWATER

HORIZON) NATURAL RESOURCE DAMAGE ASSESSMENT Version 4.0 Prepared for:

U.S. Department of Commerce National Oceanic and Atmospheric Administration, https://pub-data.diver.orr.noaa.gov/field-https://doi.org/10.1371/journal.pone.0235167 https://pub-data.diver.orr.noaa.gov/field-files/MC%20252%20Analytical%20QAP%20V4%200%20FINAL.pdf files/MC%20252%20Analytical%20QAP%20V4%200%20FINAL.pdf

D. Scope:

The USGS, NOAA, and partners must determine current baseline information on sediment communities and their geochemical environment to inform the Mesophotic and Deep Benthic Communities Habitat Assessment and Evaluation project as part of the Natural Resource Damage Assessment Open Ocean TIG. Analysis of sediment hydrocarbons, including total hydrocarbons and PAHs, and trace metals are a major factor in determining the long-term impacts from the DWH oil spill and critical to understanding overall recovery trajectories. Our objectives are to:

(1) Quantitatively assess deep GOM sediments for total hydrocarbons, PAHs, and trace metals. These results will be used to quantitatively assess deep GOM sediments for macrofauna, meiofauna, and microbial communities; assess sediment communities in relation to sediment geochemistry parameters; and compare new results with previous studies to determine the status and recovery trajectory of deep GOM sediment communities.

(2) Initial request for up to N=152 samples, with option for additional N=72 in FY25.

II. WORK REQUIREMENTS

A. Technical Requirements.

1. Specific work requirements and/or work products

a. Hydrocarbons analyses, including PAHs, saturated hydrocarbon and total extractable hydrocarbons. Sediment samples have been collected by USGS and will be shipped to the contractor.

i. PAHs including alkyl homologues by gas chromatography with low resolution mass spectrometry using selected ion monitoring (GC/MS-SIM). The analytical procedure is based on EPA Method 8270D with the GC and MS operating conditions optimized for separation and sensitivity of the target analytes.

Target PAH included in ToxPAH50 shown in Table 1 along with the Target Method Detection Limit.

ii. Saturated hydrocarbons by gas chromatography with flame ionization detection (GC/FID) based on EPA Method 8015. See Table 2 for compound details and detection limits.

iii. Total Extractable Hydrocarbons (TEH) representing the total aromatic and aliphatic hydrocarbon content of sample extracts after silica gel clean-up and analysis by GC/FID. The result is reported based on integration of the FID signal over the entire hydrocarbon range from n-C9 to n-C44 and calibrated against the average alkane hydrocarbon response factor. See Table 2 for details and detection limits.

iv. Wet sediment samples range in size from 32-36 mL (1-cm vertical fractions) and 160-180 mL (5-cm vertical fractions) and all hydrocarbon analyses need to come from an individual sample. Total number of samples is not to exceed N=152 in Y1 and an option for up to N=72 in Y2. Payment only provided for number of samples analyzed.

Table 1. List of required individual compounds of PAHs with notes about acceptable coelutions.

Target Method Detection Limit Range for Sediment/Soil = 0.1 – 0.5 ng/g dry weight.

Reference for Compound 1 chemname chemcode ToxPAH502 Alklyated PAH - Table 1.1a AQAP Naphthalene NAPTHALENE X Alklyated PAH - Table 1.1a AQAP C1-Naphthalenes C1NAPHTHS X Alklyated PAH - Table 1.1a AQAP C2-Naphthalenes C2NAPHTHS X Alklyated PAH - Table 1.1a AQAP C3-Naphthalenes C3NAPHTHS X Alklyated PAH - Table 1.1a AQAP C4-Naphthalenes C4NAPHTHS X Alklyated PAH - Table 1.1a AQAP Biphenyl BIPHENYL X Alklyated PAH - Table 1.1a AQAP Dibenzofuran DIBNZFURAN X Alklyated PAH - Table 1.1a AQAP Acenaphthylene ACENAPTYLE X Alklyated PAH - Table 1.1a AQAP Acenaphthene ACENAPTHEN X Alklyated PAH - Table 1.1a AQAP Fluorene FLUORENE X Alklyated PAH - Table 1.1a AQAP C1-Fluorenes C1FLUORENS X Alklyated PAH - Table 1.1a AQAP C2-Fluorenes C2FLUORENS X Alklyated PAH - Table 1.1a AQAP C3-Fluorenes C3FLUORENS X Alklyated PAH - Table 1.1a AQAP Anthracene ANTHRACENE X Alklyated PAH - Table 1.1a AQAP Phenanthrene PHENANTHRN X Alklyated PAH - Table 1.1a AQAP C1-Phenanthrenes/anthracenes C1PHENANCS X Alklyated PAH - Table 1.1a AQAP C2-Phenanthrenes/anthracenes C2PHENANCS X Alklyated PAH - Table 1.1a AQAP C3-Phenanthrenes/anthracenes C3PHENANCS X Alklyated PAH - Table 1.1a AQAP C4-Phenanthrenes/anthracenes C4PHENANCS X Alklyated PAH - Table 1.1a AQAP Dibenzothiophene DIBNZTHIO X Alklyated PAH - Table 1.1a AQAP C1-Dibenzothiophenes C1DBZTHIOP X Alklyated PAH - Table 1.1a AQAP C2-Dibenzothiophenes C2DBZTHIOP X Alklyated PAH - Table 1.1a AQAP C3-Dibenzothiophenes C3DBZTHIOP X Alklyated PAH - Table 1.1a AQAP C4-Dibenzothiophenes C4DBZTHIOP X Alklyated PAH - Table 1.1a AQAP Benzo(b)fluorene BNZFL_23 X Alklyated PAH - Table 1.1a AQAP Fluoranthene FLUORANTHN X Alklyated PAH - Table 1.1a AQAP Pyrene PYRENE X Alklyated PAH - Table 1.1a AQAP C1-Fluoranthenes/pyrenes C1FLUORPYR X Alklyated PAH - Table 1.1a AQAP C2-Fluoranthenes/pyrenes C2FLUORPYR X Alklyated PAH - Table 1.1a AQAP C3-Fluoranthenes/pyrenes C3FLUORPYR X Alklyated PAH - Table 1.1a AQAP C4-Fluoranthenes/pyrenes C4FLUORPYR X Alklyated PAH - Table 1.1a AQAP Naphthobenzothiophene NTHPHNE X Alklyated PAH - Table 1.1a AQAP C1-Naphthobenzothiophenes C1NTHPHNE X Alklyated PAH - Table 1.1a AQAP C2-Naphthobenzothiophenes C2NTHPHNE X Alklyated PAH - Table 1.1a AQAP C3-Naphthobenzothiophenes C3NTHPHNE X Alklyated PAH - Table 1.1a AQAP C4-Naphthobenzothiophenes C4NTHPHNE X Alklyated PAH - Table 1.1a AQAP Benzo(a)anthracene BAA X Alklyated PAH - Table 1.1a AQAP Chrysene + Triphenylene a CHRY3PHYL X alternative isomeric reference - see footnotes Chrysene a CHRYSENE

Alklyated PAH - Table 1.1a AQAP C1-Chrysenes C1CHRYSENS X Alklyated PAH - Table 1.1a AQAP C2-Chrysenes C2CHRYSENS X Alklyated PAH - Table 1.1a AQAP C3-Chrysenes C3CHRYSENS X Alklyated PAH - Table 1.1a AQAP C4-Chrysenes C4CHRYSENS X Alklyated PAH - Table 1.1a AQAP Benzo(b)fluoranthene b BBF X alternative isomeric reference - see footnotes Benzo(b+j)fluoranthene b BBJF

Alklyated PAH - Table 1.1a AQAP Benzo(j+k)fluoranthene c BJKF X alternative isomeric reference - see footnotes Benzo(k)fluoranthene c BKF Alklyated PAH - Table 1.1a AQAP Benzo(a)fluoranthene BAF X Alklyated PAH - Table 1.1a AQAP Benzo(e)pyrene BEP X Alklyated PAH - Table 1.1a AQAP Benzo(a)pyrene BAP X Alklyated PAH - Table 1.1a AQAP Indeno(1,2,3-c,d)pyrene ICDP X alternative isomeric reference - see footnotes Dibenzo(a,h+a,c)anthracene d BANTH_AHAC X Alklyated PAH - Table 1.1a AQAP Benzo(g,h,i)perylene BGHIP X

Footnotes a "Chrysene" and "Chrysene + Triphenylene" represent the same PAH. Most references, and some laboratories working under different analytical conditions, refer only to evaluation of Chrysene; however, as samples were analyzed under the DWH AQAP, the analytical laboratories found that Triphenylene coelutes with the it's Chrysene isomer; therefore, the labs, for accuracy in reporting, changed reporting "Chrysene" to "Chrysene + Triphenylene".

b "Benzo (b) fluoranthene" and "Benzo (b+j) fluoranthene" represent the same PAH. Most references, and some laboratories working under different analytical conditions, refer only to evaluation of Benzo (b) fluoranthene; however, as samples were analyzed under the DWH AQAP, some of the analytical laboratories found that the Benzo(j)fluoranthene isomer coelutes with the Benzo (b) fluoranthene isomer; therefore, some labs, for accuracy in reporting, changed reporting "Benzo (b) fluoranthene" to "Benzo (b+j) fluoranthene".

c "Benzo (j+k0) fluoranthene" and "Benzo (k) fluoranthene" represent the same PAH. Most references, and some laboratories working under different analytical conditions, refer only to evaluation of Benzo (k) fluoranthene; however, as samples were analyzed under the DWH AQAP, some of the analytical laboratories found that the Benzo (j) fluoranthene isomer coelutes with the Benzo (k) fluoranthene isomer; therefore, some labs, for accuracy in reporting, changed reporting "Benzo (k) fluoranthene" to "Benzo (j+k) fluoranthene".

d "Dibenzo (a,h) anthracene" and "Dibenzo (a,h+a,c) anthracene" represent the same PAH.

Most references, and some laboratories working under different analytical conditions, refer only to evaluation of Dibenzo (a,h) anthracene; however, as samples were analyzed under the DWH AQAP, some of the analytical laboratories found that the Dibenzo (a,c) anthracene isomer coelutes with the Dibenzo (a,h) anthracene isomer; therefore, some labs, for accuracy in reporting, changed reporting "Dibenzo (a,h) anthracene" to "Dibenzo (a,h+a,c) anthracene".

REFERENCES

1 Analytical Quality Assurance Plan (AQAP), Mississippi Canyon 252 (Deepwater Horizon) Natural Resource Damage Assessment, Version 3.1, Prepared for NOAA by EcoChem, Inc.

November 15, 2012

2 ToxPAH50 is a list of 50 PAHs which Stratus Consulting uses to evaluate the PAH content of samples (Memorandum, 12/7/12)

Table 2. Saturated Hydrocarbons (Alkanes/Isoprenoids Compounds) and Total Extractable Hydrocarbons.

Abbr. Analyte Abbr. Analyte nC9 n-Nonane nC23 n-Tricosane nC10 n-Decane nC24 n-Tetracosane nC11 n-Undecane nC25 n-Pentacosane nC12 n-Dodecane nC26 n-Hexacosane nC13 n-Tridecane nC27 n-Heptacosane 1380 2,6,10 Trimethyldodecane nC28 n-Octacosane nC14 n-Tetradecane nC29 n-Nonacosane 1470 2,6,10 Trimethyltridecane nC30 n-Triacontane nC15 n-Pentadecane nC31 n-Hentriacontane nC16 n-Hexadecane nC32 n-Dotriacontane nPr Norpristane nC33 n-Tritriacontane nC17 n-Heptadecane nC34 n-Tetratriacontane Pr Pristane nC35 n-Pentatriacontane nC18 n-Octadecane nC36 n-Hexatriacontane Ph Phytane nC37 n-Heptatriacontane nC19 n-Nonadecane nC38 n-Octatriacontane nC20 n-Eicosane nC39 n-Nonatriacontane nC21 n-Heneicosane nC40 n-Tetracontane nC22 n-Docosane

TEH

Σ(C9-C44) Integration of the FID signal over the entire hydrocarbon range from n-C9 to n-C44 after silica gel cleanup.

TEM

Σ(C9-C44) Integration of the FID signal over the entire hydrocarbon range from n-C9 to n-C44 no silica gel cleanup.

Target Method Detection Limit

Sediment (Alkanes) = 0.01 μg/g dry weight Sediment (TEH) = 1 μg/g dry weight Water (Alkanes) = 0.8 µg/L

Target Reporting Limit Oil (Alkanes) = 200 mg/kg

Oil (TEH) = 200 mg/kg Water (TEH/TEM) = 200 µg/L

TEH = Total Extractable Hydrocarbons with silica gel "clean-up" TEM = Total Extractable Matter with no extract "clean-up"

b. Trace Metals analyses. Sediment samples have been collected by USGS and will be provided to the contractor.

i. Total metals in sediments and tissues by inductively coupled plasma atomic emission spectrometry (ICP-AES) and inductively coupled plasma-mass spectrometry (ICP-MS) and total mercury in sediments and tissues by cold vapor atomic absorption (CVAA) or cold vapor atomic fluorescence spectrometry (CVAFS). The analytical procedures are based on EPA SW-846 Methods 6010C, 6020A, 7470A, 7471A, 7471B, 7474, and 7742. The target analyte list and target reporting limits (RLs) for each matrix are included in Table 3 and must include aluminum and barium.

ii. Original wet sediment samples range in size from 32-36 mL (1-cm vertical fractions) and 160-180 mL (5-cm vertical fractions) and all hydrocarbon analyses need to come from an individual sample. Total number of samples is not to exceed N=152 in Y1 and an option for up to N=72 in Y2. Payment only provided for number of samples analyzed.

Table 3. List of target metal analytes and reporting limits.

Metals Target Analyte List

Analyte

Method

Target Reporting Limits (RL)

Sediment (mg/Kg) dry weight

Tissues (mg/Kg) wet weight Aluminum ICP/ICP-MS 10 5 Antimony ICP/ICP-MS 0.05 NA Arsenic ICP-MS 0.5 0.5 Barium ICP/ICP-MS 0.1 0.05 Beryllium ICP/ICP-MS 0.05 NA Cadmium ICP/ICP-MS 0.05 0.02 Calcium ICP/ICP-MS 100 NA Chromium ICP/ICP-MS 0.2 0.2 Cobalt ICP/ICP-MS 0.05 0.05 Copper ICP/ICP-MS 0.1 0.1 Iron ICP/ICP-MS 10 2 Lead ICP/ICP-MS 0.05 0.02 Magnesium ICP/ICP-MS 50 NA Manganese ICP/ICP-MS 0.2 0.2 Mercury CVAA/CVAFS 0.01 0.01 Nickel ICP/ICP-MS 0.5 0.5 Potassium ICP/ICP-MS 100 NA Selenium ICP/ICP-MS 0.1 0.1 Silver ICP/ICP-MS 0.05 0.05 Sodium ICP/ICP-MS 100 NA Strontium ICP/ICP-MS 2.00 NA Thallium ICP/ICP-MS 0.1 NA Vanadium ICP/ICP-MS 0.5 0.5 Zinc ICP/ICP-MS 1 0.5

Method detection limits (MDL) should be at least 3 times lower than the target reporting limits.

c. Report of quantitative results of hydrocarbons and trace metals including quality assurance and quality control parameters.

2. Criteria for acceptance of Product or Service

a. All analyses must be consistent with the MC252 Analytical Quality Assurance

Plan

b. Report includes quantitative measures of hydrocarbons and trace metals including quality assurance and quality control parameters.

B. Deliverables.

1. Characteristics for work product described in II.A.1., identified by name and quantity.

a. Quantitative measures of identified PAHs, saturated hydrocarbons, and total hydrocarbons in excel spreadsheet or other machine-readable format (e.g. .csv)

b. Quantitative measures of trace metals in excel spreadsheet or other machine-readable format (e.g. .csv).

c. Data should be in a format suitable for a data release or online data repository.

2. Delivery dates for work/product to be completed and point of delivery.

a. Final data to be delivered within 120 of receipt of samples by vendor

b. Delivered to USGS Wetland and Aquatic Research Center, Gainesville, FL

3. Any unused portions of samples will be returned to USGS Wetland and Aquatic

Research Center, Gainesville, FL in case additional analyses are identified at some future time.

III. SUPPORTING INFORMATION

A. Place of Performance. Contractor laboratory

B. Government Furnished Property (GFP): USGS will provide sediment samples for chemical analyses

C. Special Considerations.

1. Key personnel qualifications: Contractor must have demonstrated ability to conduct the analyses required, as demonstrated by published reports of similar studies.

2. Data Rights, Safety, Environment: USGS Wetland and Aquatic Research Center will retain rights to the data resulting from these analyses and will follow established USGS protocols for releasing data when publishing results of this study.

3. Security/Privacy issues: N/A