Attachment - 1 Statement of Work.pdf

Attachment 1

STATEMENT OF WORK (SERVICES)

(SOW)

GENERAL INFORMATION

Title of Project: Detection of global proteins and mRNA in postnatal heart targeted AdCy8 overexpression mouse strain.

Statement of Need and Purpose: The purpose of this project is to study postnatal protein expression and mRNA signals in AC8 models in order to illucidate their pathways and their mechanisms in heart cell survival, cell renewal and cell mitosis and its regulation.

Background Information and Objective: The overexpression of adenylyl cyclase in the mouse heart produces a strong beating heart with the upregulation of many pathways. Global analysis of proteins that emerge are central for the heart survival and regulation of the cell cycle and mitosis check points.

NIA plans to scan global proteins and mRNA by detecting its binding partners in the AdCy8 overexpression model. This analysis will be done through the use of a special method called immunoprecipitation (IP) followed by mass spectrometry analysis (MS), mRNA seq and comparison of the results with the wild type mouse model.

Period of Performance: The anticipated period of performance is for 6 months as follows:

September 23, 2024 - March 22, 2025

SCOPE OF WORK

General Requirements:

Independently and not as an agent of the Government, the Contractor shall furnish all the necessary services, qualified personnel, material, equipment, and facilities, not otherwise provided by the Government as needed to perform the Statement of Work.

Specific Requirements:

• Method development and testing of Immuno precipitation (IP) (24 samples), MS analysis (16 samples) and mRNA seq (24 samples). The requirement for IP-LC/MS is to provide biochemical identification, relative quantification, and statistical analysis with data visualization for each sample. mRNA seq requirement is to provide multiomics associations with numerous analytes that are distributed across the different biochemical classes including small molecules, lipids and proteins, using our small sample size, demonstrating high signal-to-noise ratios.

GOVERNMENT RESPONSIBILITIES: Deliver 24 AC8 tissue samples for analysis

DELIVERY OR DELIVERABLES

Global-proteomics analysis output including:

Attachment 1

• Raw LC-MS data

• Table of LC-MS features each with: Relative peak area quantification among samples

• Putative bioinformatic identifications

• Association statistics with provided sample variables

• Data visualizations Global data structure ▪ Dimensionality reduction

• Biological associations ▪ Volcano plots

• Individual analyte associations

• mRNA Sequencing Output:

• RNA extraction

• QC

• Library preparation

• RNA sequencing

• Data analysis

REPORTING REQUIREMENTS: Deliverables must be received within 90 days after receipt of samples. Results will include raw data, table of LC/MS features, bioinformatic identifications, Data visualizations Global data structure , Dimensionality reduction, Volcano plots, analyte associations.

OTHER CONSIDERATIONS

Travel: None

Key Personnel: None

Data Rights: All Data produced will be the sole property of NIH

Section 508 Electronic and Information Technology Standards: Not required.