Attachment 1_Statement of Need (SON) _Cell Analyzers.pdf
PDF 129 KB Posted
- Attached to
- Cell Analyzers– Brand Name or Equal Federal contract opportunity
- Solicitation number
- RFQ-NIAID-24-2225539
About this file
This document is a Statement of Need (SON) and a related federal contract opportunity for the procurement of eight (8) cell analyzers for the National Institute of Allergy and Infectious Diseases (NIAID), Vaccine Research Center (VRC), Flow Cytometry Core Facility (FCC). The SON outlines the minimum technical requirements for the cell analyzers, including the need for at least 5 lasers, 50 simultaneous parameter detection, LED pulser technology, high throughput sampler, and compatibility with existing instruments and software.
The federal contract opportunity is a Request for Quotes (RFQ) with a brand name or equal purchase description. NIAID seeks to acquire the cell analyzers to maintain continuity of science, data integrity, and experimental compatibility with existing equipment at the VRC. The requirement is being competed with full and open competition, with a 12-month warranty period. Quotes are due by September 13, 2024, and NIAID will evaluate them based on technical acceptability, price, and past performance to determine the best value.
View the file
Other files for this federal contract opportunity
| File | Type | Posted |
|---|---|---|
| Attachment 2_BOM_Cell Analyzer.pdf | ||
| Attachment_3_Cell Analyzers - RFQ-NIAID-24-2225539 - Full Text Clauses.pdf |
On GovTribe
Work with this file on GovTribe
- Download the original file
- Contacts named in this file
- Similar government files
- Ask GovTribe AI about this file
Text version
Attachment #1
Statement of Need (SON)/Minimum Technical Requirements for Cell Analyzers
This statement of need is for the procurement of eight (8) cell analyzers that must have the following minimum salient capabilities/characteristics:
• Must include at least 5 lasers in the standard configuration with the potential of adding at least 4 additional lasers.
o This feature allows the advancement to more parameters, if necessary, without the purchase of new instrumentation, therefore providing a built-in future expansion as experiments expand. At the Vaccine Research Center (VRC), this allows for multidimensional cell sorting for cells involved in protective immunity.
• Must contain at least 50 simultaneous parameter detection of 48 fluorescence markers plus forward scatter (FSC) and side scatter (SSC) signals. Upgradable to at least 60 parameters with increased laser capability.
o This provides the ability to sort complex cell clusters in 50 parameter space and to match panels currently measured on existing 30 parameter cell analyzers and cell sorters that will be required to sort the same number of parameters (i.e., 48 fluorescence colors plus two physical parameters). At the VRC, this allows for multidimensional cluster cell sorting for cells involved in protective immunity.
• Must include a LED pulser technology or equivalent measurements for Quality Control (QC) by measuring relative background using automated voltage ranging.
o This feature allows for rapid instrument setup and inter and intra instrument comparison, which are required for large research or clinical experiments. This improves more accurate quality control for increased precision and reproducibility of data.
• Must include a high throughput sampler (HTS) robotics.
o This device will allow 96 well plates (and other plate formats) to be used and sampled directly into the cell analyzer to allow for faster data collection speeds.
o Plates used in processing can be directly used on the HTS and therefore can reduce potential transfer errors.
• Must use a high-quality photomultiplier tube (PMT), known as the GaASP and GaA. These detectors are a proven technology and are compatible with our existing instruments and are currently used research cell panels developed in prototype instrument used at the VRC.
Therefore, these detectors are compatible with our existing instruments and previous panel development, requiring minimal impact on existing panels and future panels.
• Requires the use of standard FACS-DIVA software, which is compatible with existing instruments, including the S6 cell sorter. This workflow pipeline is required for continuity of cellular and genetic experiments.
• Must process and be compatible with Flow Cytometry Standard files in version.3.1 or higher for use in the FlowJo data analysis software for standard and multidimensional data analysis.
• Must include a twelve (12) month warranty that will begin upon installation or thirty days after the date of shipment, whichever occurs first.
File details come from the government source that posted it. Updated .