Attachment 1 - SPT Labtech Purchase Description_Final.pdf
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- Attached to
- Automated Cryo-EM Sample Preparation Instrument
- Solicitation number
- 75P00125Q00071
About this file
This document is a Purchase Description for an Automated Cryo-EM Sample Preparation Instrument, issued by the Structural Biology Core (SBC) of the Vaccine Research Center at the National Institute of Allergy and Infectious Diseases (NIAID), National Institutes of Health (NIH). The procurement includes one Chameleon Cryo-EM Sample Preparation Instrument with specific accessories, such as two Cryogen Bowl Assemblies, an Ethane Condensing Kit, a Desktop PC Controller, and a Consumables Starter Pack. The instrument is designed for high-resolution cryo-electron microscopy sample preparation, with capabilities including sample temperature control (4°C to 40°C), batch capacity of 8 grids, and integrated features like liquid nitrogen level monitoring and a closed-loop glow discharge module.
The purchase description specifies delivery within 30 days after receipt of order and includes a 12-month warranty covering replacement of defective components due to manufacturing or design defects. The instrument will support the SBC's research in developing antibody therapeutics and vaccines for infectious diseases, including COVID-19, HIV-1, malaria, flu, and RSV, by enabling more consistent and higher-quality sample preparation compared to manual plunging techniques. The total quantity requested is one instrument with associated accessories and consumables, tailored to meet the specialized needs of the Vaccine Research Center's structural biology research.
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Attachment 1
PURCHASE DESCRIPTION
1. Statement of Need and Purpose: Automated Cryo-EM Sample Preparation Instrument
2. Background Information and Objective: The Structural Biology Core (SBC), Virology Laboratory (VL), Vaccine Research Center (VRC), National Institute of Allergy and Infectious Diseases (NIAID), National Institutes of Health (NIH) uses a structure-based approach for developing antibody therapeutics and vaccines for infectious diseases, including but not limited to COVID-19, HIV-1, malaria, flu, and RSV. The structural analysis of pathogen proteins, antibodies, and vaccines is essential to current and future SBC research. It requires high-resolution cryo-EM data attainable only with high-quality samples. Currently, SBC relies on manual plungers for the preparation of cryo-EM grids, which can yield suboptimal results, particularly with challenging samples. The adoption of automated, non-blotting technology offers a promising solution to this issue. To meet the specialized needs of SBC VRC, SBC needs to acquire the Chameleon Cryo- EM Sample Preparation Instrument.
3. Generic Name of Product: Automated Cryo-EM Sample Preparation Instrument
4. Purchase Description:
• Qty(1) - Chameleon Cryo-EM Sample Preparation Instrument
• Qty(2) - Chameleon Cryogen Bowl Assembly
• Qty(1) - Ethane Condensing Kit
• Qty(1) - Chameleon in-built Desktop PC Controller (Win10) with Chameleon Software
License & Comms Installed
• Qty(1) - Chameleon Consumables Starter Pack Includes:
- 4150-40001 - pack of 50
- 4150-40003
- 4150-40004
- 4150-40005 - pack of 50
- 4150-40010
- Types Each
- 4150-40011
- NPN Source Bottles (Working Fluid Bottle and Waste)
• Qty(1) - Chameleon Installation
• Qty(1) - Vision lite - Chameleon Applications Support Package
5. Salient characteristics:
- Minimum Sample Volume: 5μL Minimum Dispense Volume: 6nL
- Batch Capacity: 8 Grids
- Sample Temperature Control: 4°C to 40°C Minimum Dispense-to-Plunge Time: 54 msec
Liquid Nitrogen Level Monitoring
- Liquid Ethane Temperature Control
- Integrated Semi-Automated Characterization and Plunge Speed Tuning
- Integrated Closed Loop Glow Discharge Module Integrated Post-Dispense QC System
- Session Tracking and Reporting Datapack
6. Delivery: Delivery shall occur within thirty (30) days after receipt of order (ARO).
Attachment 1
7. Quantity: One (1) instrument with associated accessories and consumables as listed above,
8. Warranty: Warranty coverage shall extend for 12 months from Government acceptance and include replacement of defective components due to faulty manufacture or design defects.
File details come from the government source that posted it. Updated .