Attachment 1 - Specs and Requirements - Seq Svcs Honeybees.pdf
PDF 387 KB Posted
- Solicitation number
- 1159732
About this file
This document is a Specifications and Requirements document for Sequencing Services focused on Stressors in Honeybee Colonies. The service involves processing 357 total RNA samples from honey bees, with sequencing specifications including Illumina 2x150bp configuration, ~20 million reads per sample, and a quality guarantee of ≥85% of bases at ≥Q30. The comprehensive service covers sample QC, library preparation, sequencing, and data analysis, with deliverables including quality reports, FASTQ files, data QC, trimming, mapping, differential gene expression, alternative splicing, and gene ontology analysis.
Sample requirements specify total RNA must be submitted in nuclease-free water on dry ice, with a requested submission of ≥500 ng in ≥25µl at a concentration of ≥20 ng/µl. Samples must meet quality standards including RIN >6.0, A260/A280 ratio of 1.8-2.2, and be DNA-free. For projects with 16 or more samples, samples should be prepared in 96-well full-skirted PCR v-bottom plates, with delivery scheduled 45 days after receipt of order. The service is designated for research use only, with additional benefits including updates at completed project milestones.
View the file
Other files
| File | Type | Posted |
|---|---|---|
| Single Source Justification Less than SAT - Seq Svc Bees_Redacted.pdf |
On GovTribe
Work with this file
- Download the original file
- Contacts named in this file
- Similar government files
- Ask GovTribe AI about this file
Text version
Specifications and Requirements for Sequencing Services – Stressors in Honeybee Colonies
Service Quantity Standard RNA-seq bundle, no enrichment, 20M paired-end reads per sample 263
Standard RNA-Seq analysis, value package 263 Standard RNA-seq bundle with PolyA selection, 30M paired-end reads per sample (project #30-1230782813) 263
Standard RNA-Seq analysis, value package (project #30-1230782813) 263
Non-human whole genome sequencing bundle, 2x150bp, 10 Gb per sample, value package (project #30-1230782113) 263
Whole Genome Metagenomics analysis, value package (project #30-1230782113) 263
Data delivery, customer cloud -
Description
Service Type Standard RNA-Seq Species Honey Bee Sample Type Total RNA (not derived from blood) Sample Number 357 sample(s) Sequencing Configuration Illumina, 2x150bp Data Output ~20 million reads per sample Phi-X Spike-in n/a Quality Guarantee ≥85% of bases ≥Q30
Scope of Work
Sample QC, Library Preparation, Sequencing, and Data Analysis
For information on processing workflows, guarantees, sample or data retention policies, or recommendations, please refer to the NGS FAQ.
Deliverables
Quality report, FASTQ files, data QC, trimming, mapping, differential gene expression, alternative splicing, and gene ontology analysis
Gene ontology analysis is only available for species in the following link. If species is not listed, Azenta reserves the right to exclude gene ontology analysis from the final report.
Level of Service Research Use Only
Additional Benefits Updates at completed project milestones (Extraction, QC, Library Preparation, Sequencing, and Data Analysis, if applicable)
357 total RNA samples (not derived from blood) meeting requirements below.
II. Sample Requirements
Sample Type Remarks Quantity and Concentration Quality
Total RNA (in nuclease-free water), on dry ice
Requested Submission
≥500 ng in ≥25µl Concentration: ≥20 ng/µl RIN >6.0, A260/A280=1.8-2.2, DNA-free
Absolute Minimum Submission
≥150 ng in ≥25µl Concentration: ≥6 ng/µL
Tubes: Prepare samples in clearly labeled and well-organized 1.5 mL flip-cap microcentrifuge tubes.
Please avoid using Parafilm to seal the tubes.
Plates: For projects with 16 or more samples, prepare samples in clearly labeled, securely sealed 96-well full-skirted PCR v-bottom plates. Arrange the samples vertically by column (i.e. A1, B1, C1, etc.)
Delivery: 45 days after receipt of order (ARO)
(End)
File details come from the government source that posted it. Updated .