Attachment 1 - Specifications - 1232SA26Q1362.pdf
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- 1232SA26Q1362
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This document contains technical specifications for ThermoFisher Scientific consumables compatible with the SeqStudio Genetic Analyzer instrument platform.
The SeqStudio™ Cartridge v2 is a 96-well plate or 8-tube strip format cartridge with a 28 cm capillary length designed for multiple sequencing applications including rare variant sequencing, resequencing, comparative sequencing for somatic mutations, fragment analysis, short read sequencing, de novo sequencing, and short PCR product sequencing. Each cartridge measures 49.5 cm (length) × 64.8 cm (width) × 44.2 cm (height) and provides sufficient material for up to 250 injections or 1000 reactions on a 4-capillary instrument. The cartridge must be shipped on wet ice and stored in a refrigerator at 2–8°C. The BigDye™ Terminator v3.1 Cycle Sequencing Kit contains 1000 reactions and is suitable for de novo sequencing, resequencing, and finishing applications using PCR products, plasmids, fosmids, and BAC templates. The kit is compatible with multiple sequencing instruments (SeqStudio™, SeqStudio™ Flex Series, 3500/3500xL, and 3730/3730xl analyzers) and thermal cyclers (GeneAmp™ PCR System 9700 Dual Series and Veriti™ Fast thermal cyclers). Kit contents include 10 tubes of BigDye™ Terminator v3.1 Ready Reaction Mix, M13 (-21) Primer, pREF-BDT™ Control DNA, and 12 mL of 5X Sequencing Buffer. The kit must be stored at -15°C to -25°C and protected from repeated freeze-thaw cycles.
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Specifications ThermoFisher Scientific consumables
SeqStudio Genetic Analyzer instrument consumables
Specifications
SeqStudio™ Cartridge v2
• Capillary Length: 28 cm
• For Use With (Application): Rare Variant Sequencing, Resequencing, Comparative Sequencing
(Somatic Mutations - 10:50), Fragment Analysis (Denaturing), Short Read Sequencing, De Novo Sequencing - Low to Mid Throughput, Short PCR Product Sequencing
• Format: 96-well Plate, 8-tube Strip
• Height (Metric): 44.2 cm
• Length (Metric): 49.5 cm
• Product Type: Cartridge V2
• Quantity: Up to 250 Injections or 1000 Reactions
• Shipping Condition: Wet Ice
• Throughput: 4 Capillary Instrument
• Width (Metric): 64.8 cm
• Product Line: SeqStudio
• Unit Size: Each
• Contents & Storage: Sufficient for 250 injections/1000 reactions. Store in refrigerator (2–8°C).
BigDye™ Terminator v3.1 Cycle Sequencing Kit
• For Use With (Application): Ideal for De Novo Sequencing, Resequencing and Finishing with PCR Product, Plasmid, Fosmid and BAC templates
• For Use With (Equipment): SeqStudio™ Genetic Analyzer, SeqStudio™ Flex Series Genetic Analyzer, 3500/3500xL Genetic Analyzer, 3730/3730xl DNA Analyzer Thermal Cyclers:
GeneAmp™ PCR System 9700 Dual Series, Veriti™ Fast 96‐well and 384‐well Thermal Cyclers
• Product Line: BigDye™ Terminator
• Product Type: Sequencing Buffer
• Quantity: 1000 Reactions
• Technique: Fluorescent Dye Terminator Sequencing
• Template Compatibility: BAC DNA, Lambda DNA, Genomic DNA (Bacterial), Rolling Circle
Amplified Products, Fosmids, PCR Amplicons (50:50 Heterozygotes), Plasmid DNA (≤15 Kb), Single Stranded DNA
• Format: 12-well Strips, 8-well Strip, 96- or 384-well Plate
• Unit Size: Each
• Contents & Storage: 10 tubes of BigDye™ Terminator v3.1 Ready Reaction Mix, M13 (-21)
Primer, pREF-BDT™ Control DNA, 1 x 12 mL tube of 5X Sequencing Buffer (may be stored at 4°C).
Store kit at -15°C to -25°C and avoid repeated freeze-thaw cycles.
--End Specifications--
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