Attachment 1 - Specifications (1232SA26Q0353).pdf

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USDA-SEA Long-Read Large Genome Sequencer Federal contract opportunity
Solicitation number
1232SA26Q0353
Issued by
Department of Agriculture Agricultural Research Service

About this file

This document is a technical specifications attachment for a long-read large genome sequencer procurement. The government seeks a long-read sequencing instrument capable of sequencing larger genomes (animal genomes) with the capacity to produce 90 GB of sequencing data. The instrument must meet specific technical performance standards including the ability to sequence reads up to a minimum of 20 kilobase fragments, sequence smaller fragments for transcriptome studies, and detect 5-methylcytosine (5mC) and 5-hydroxymethylcytosine (5hmC) modifications in DNA sequences. Data output must be generated in FASTQ and/or BAM formats.

The procurement includes mandatory training and reagents for training as part of the purchase. The instrument does not require its own computer infrastructure due to existing on-site computing resources. Delivery must be completed no later than 60 days after contract award to Nick Chaplinski/Tim Satterlee at 950 College Station Road, Athens, GA 30605. No pricing terms, response dates, award dates, federal agency information, or contract vehicle type are specified in this specifications document.

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Attachment 1 - Specifications Long-Read Large Genome Sequencer

Technical Requirements: Long-read sequencing instrument that can sequence larger genomes (i.e. animal) and produce 90 GB of sequencing data.

• The sequencer must be capable of sequencing reads up to a minimum of 20kb fragments.

• The sequencer needs to be able to sequence smaller fragments for other types of analysis like transcriptome studies.

• Instruments must be able to detect 5-methylcytosine (5mC) and 5-hydroxymethylcytosine

(5hmC) modifications in DNA sequences.

• Due to high computing power already available on-site, the instrument does not need its own computer infrastructure.

• Data generated must be produced in either FASTQ and/or BAM formats.

• Training in the use of the instrument must be included with its purchase and provide reagents for the training.

• Period of performance is not later than 60 days after award.

Delivery address:

Nick Chaplinski/Tim Satterlee 950 College Station Road Athens, GA 30605

File details come from the government source that posted it. Updated .