Attachment 1_Specification Requirements for Capillary Gel Electrophoresis Unit for Protein Analysis.pdf

Item Description:

Capillary Gel Electrophoresis Unit for Protein Analysis (Instrument & Software) – Abby System - Brand

Name Or Equal Product.

Product Specifications:

• Bench-top footprint with dimensions not exceeding 36 cm H x 36 cm W x 57 cm D

• Weight not to exceed 23 kg

• Fully operable requiring only a single universal voltage (100VAC-240VAC, 50/60 Hz) power output connection

• Fully operable in a humidity range of 20-60% relative, non-condensing

• Fully operable in a temperature range of 18-24oC

• Processes a maximum of 25 samples per run

• Run time <3 hours, <5 with RePlex

• Uses capillaries with a length of 5 cm

• Uses capillaries with a volume capacity of 400 nL

• Uses capillaries with a 100 μM inner diameter

Sample Input Specifications

• Process a maximum of 25 samples per run

• Store samples at room temperature

• Analyze the following samples: protein lysates from cultured mammalian cell lines, white blood cells, FACS-sorted cells, plasma, and crude cell culture supernatants

Reaction Vessel Specifications

• Fused silica capillaries that are 5 cm in length, 100 μM inner diameter and 400 nL volume capacity

• Perform protein capture using proprietary inner capillary chemistry coating that is UV light activated to cause covalent immobilization of all proteins to the inner capillary wall

Throughput Specifications

• Process up to 25 individual samples in a microtiter plate with space for separation and stacking matrices, primary and secondary antibodies, and peroxide and luminol chemiluminescent substrate which are required to carry out 25 individual immunoassays.

• Complete 25 nano-immunoassays within a cycle time in three hours

• Complete 2 rounds of Immunoprobing and detection in a single capillary (2 serial immunoassays or immunoassay + total protein assay) in five hours using RePlex.

• Robotic automated system which prepares capillaries for sample uptake

• Robotic automated system which volumetrically loads 40 nL sample and ~360 nL of buffer

• Perform automated size-based separation

• Wash capillaries, remove matrices, and perform automated immunoassay steps incubating of primary and secondary antibodies and protein blocking steps

• Automatically introduce chemiluminescent/fluorescent reagents for immunoassay detection results

Separation Specifications

• Molecular weight size separation power supply able to produce 0-3,000 volts

• Constant voltage output

Immobilization Specifications

• Software to control the UV lamp

• Contains a low-pressure mercury lamp

Detection Specifications

• Automated detection of fluorescently labeled protein size standards

• Molecular weight ladder ranges from 2 to 440 kDa

• Ability to distinctly resolve biotinylated protein standards

• Chemiluminescent detector

• CCD Camera that is cooled and with 1.4 megapixel and 16-bit resolution

Software Specifications

• Compass software to set-up assay protocols and analyze run data

• Specify up to 25 samples per run

• Specify primary and secondary antibodies per sample

• Specify the molecular weight per sample

• Specify chemiluminescence collection time

• Perform relative quantitation of identified peaks by total area or peak height