Attachment 1 SOW revised.pdf

1 | P a g e

Statement of Work (SOW)

High Performance Liquid Chromatograph – High Resolution Mass Spectrometer

The Government has a requirement for a Liquid Chromatography – Hybrid Quadrupole High-resolution Mass Spectrometer (LC-HRMS) instrument for the New York U.S. Customs and Border

Protection (CBP) Field Laboratory (NY Lab) location.

Equipment shall comprise all components necessary for chromatographic separation of compounds and high-resolution MS and MS/MS. The contractor shall provide all equipment, installation, training, and technical support necessary to meet the requirements in this document. The vendor will also be responsible for removal of the current AB SciEX 4500 QTrap system. The installed instrument system, software, and database applications shall be new, commercially available, and in commercial production as of the closing date of this announcement. The complete, installed, instrument system in the New York Laboratory shall meet the entire main and sub-system manufacturer’s published performance specifications as installed in our laboratory on our site.

Instrument requirements:

The instrument system containing all listed sub-systems and major components shall be provided as part of the complete instrument system and each shall meet their individually described detailed technical and performance specifications. The instrument system and sub-systems will be purchased from and serviced by a single vendor.

The provided instrument shall consist of the following listed major sub-systems and their listed major components. All provided specifications are minimum acceptable specifications unless otherwise indicated.

The LC-HRMS system shall meet the following specifications:

Liquid Chromatography

Vacuum Degasser

Sufficient channels for mobile phase solvents and any wash solvents used by the system

Binary pumps

High pressure (binary) gradient configuration

Capable of at least 0.01 ml/min to 2.0 ml/min

Capable of maintaining pressures of NLT 650 Bar

Flow accuracy of ±1%

Flow precision of ≤ 0.1% RSD

Auto-sampler

Capacity of NLT 48 standard 1.8 ml vials

Capable of injecting 0.1 – 25 μl with a repeatability of ≤ 0.5% RSD for partial-loop injections AND ≤ 0.3% RSD for full-loop injections; OR ≤ 0.3% RSD at 10 µL injections thermostatically controllable within a range of 4 °C

– 40 °C

Column Oven

Temperature controlled column oven with a temperature range of ambient to 80 °C

Temperature stability of 0.5 °C

2 | P a g e

The oven shall be of sufficient size to accept 250 mm columns.

General All LC components in the solvent flow path shall tolerate a minimum pH range of 2 – 9

Solvent mixer shall be included

Interface

The high-resolution mass spectrometer shall interface directly to the liquid chromatograph and be controlled by the same software package.

No third-party interface shall be necessary. No third-party software shall be used for instrument control.

High-resolution

Mass Spectrometer

The HRMS shall be a hybrid instrument consisting of a quadrupole (or equivalent) coupled to a high-resolution mass spectrometer.

Available scan types

High-resolution, accurate mass MS

MS/MS

MRM

Ion source

ESI source capable of both positive and negative ionization modes with dynamic switching

Maximum flow rate of not less than 2.0 ml/min

Option of adding an APCI source (a single source with interchangeable probes is acceptable)

Ion Source must be able to be removed without venting the instrument.

Resolution NLT 40,000 FWHM

Scan speed NLT 20 Hz

Precursor ion selection range

NLT 40-2000 m/z

HRMS

mass range

NLT 40,000 Da

Mass Accuracy

≤ 0.8 ppm RMS with internal calibration

Drift Maximum drift of 1 ppm at ±2 °C ambient temperature variation

Dynamic Range

NLT four orders of magnitude

Vacuum system

The vacuum system is automated, consisting of an air-cooled turbomolecular pump(s) and one oil-free rough pump. Vacuum read backs and system vent/pump cycles are digitally monitored and controlled.

Mass calibration/tune shall be performed internal to the instrument and controlled through software; no separate syringe pump shall be used to introduce calibrant. Tune frequency during instrument use shall be user selectable (e.g., every five injections)

3 | P a g e

The system shall also include a gas generator, sufficient to supply all necessary gases for full instrument operation; no compressed gas cylinders shall be required. The laboratory has in-house compressed air at >120 psi. Therefore, a compressor-less nitrogen generator is preferred. The system shall include an uninterruptable power supply (UPS) sufficient to power the instrument and allow for an orderly shutdown. The system shall include a power/boost transformer to boost line voltage of nominal 208V (supplied) to nominal 230V, if necessary.

The total number of required laboratory outlets, including voltage, amperage, and plug type for each shall be included in the quote.

Size Constraints

The instrument, including the LC, tandem HRMS, computer and all other quoted components

(excepting a power conditioner/UPS, rough pump, and gas generator) must fit onto a fixed bench space. The maximum installed instrument dimensions are: 120” long, 25” deep, and 70” high (to include any necessary clearance between the instrument and ceiling). Neither the ceiling nor the bench can be moved or altered in any way. The instrument may overhang on both front and rear of the bench but must otherwise sit as per manufacturer’s specifications. If any bid proposes an instrument whose dimensions are greater than the above, explicit written documentation must be provided permitting the overhang or the bid will be considered non-responsive. The instrument may not be installed in a rotated configuration solely to fit space constraints.

Software and Libraries

The operating system included with the system shall be:

• Windows 10 Professional 64-bit or newer; or

• If a non-Windows OS is specified, the documented EOL date must be included with the bid documentation, which shall be no less than 7 years from the date of acceptance.

Supplied software shall include an instrument control and data collection software package.

The instrument control software must support both data independent acquisition workflows

(DIA) and data dependent workflows (DDA). In addition to the instrument control software, software designed to support statistical analysis of multiple data sets (e.g., PCA) to correlate spectral data and user defined characteristics shall be included. This software must be able to identify spectral differences correlated with user defined information (e.g., sample class). In addition, software for library searching/matching of HRMS spectra shall be included with libraries covering, at minimum, veterinary drugs (antimicrobials), drugs of abuse (forensics), pharmaceutical compounds, and natural products. The library/libraries shall contain high-resolution MS/MS data in both positive and negative modes (where appropriate). The size of the library and the number of high-resolution MS/MS spectra contained therein shall be specified in the quote. In addition, the software shall allow for the creation of custom searchable libraries by the user. The system control software shall support both data dependent acquisition (DDA) and data independent acquisition (DIA) methods. All libraries and software must be quoted with a perpetual license.

Setup/installation and training:

Inside delivery and set-up of all purchased equipment, supplies, hardware, and software is required. The vendor shall provide software and firmware upgrades free of charge for a

4 | P a g e minimum of three years from the date of installation of the instrument. Software and firmware updates shall be applied to the instrument without the need for an internet connection.

The installation shall include a pre-installation site visit to validate site of installation, if recommended by the vendor.

An in-house training session by a qualified trainer, directly employed by the vendor, is required for the instrument upon installation. The training session will occur at the

Laboratory in New Jersey at the address shown below. The training session shall provide familiarization training for at least three staff members with the software, basic operation, set-up of user accounts, interpretation of data, other software capabilities, basic hardware maintenance, and troubleshooting. Travel/per diem fees for installation/training specialist shall be included. Further, in depth training

Warranty and updates:

The vendor shall provide warranty on the instrument for a minimum of three years from the date of installation of the instrument on all hardware and software purchased. The warranty shall include all parts, labor, and travel/per diem expenses. The warranty shall include performance of preventative maintenance as recommended by the manufacturer. On-site repair and maintenance by a qualified service engineer will be available within five business days of a service call.

Since the laboratory intends to use the instruments for approximately ten years it is the expectation of the laboratory that manufacturer provided service and spare parts will be readily available for purchase for the next ten years. On site, proof of satisfactory operation within the manufacturer’s specifications shall be provided prior to acceptance of the instruments.

Instrument Removal / Trade-In Requirements:

Vendor shall be responsible for removal (and/or accept via trade-in) the current AB SciEX

4500 QTrap being replaced. Vendor is responsible for including a line item of applicable trade-in credits (if any) being deducted from the total instrument purchase cost on the bid.

Vendor shall provide all necessary packaging/crates, removal labor/shipping/insurance, and anything else needed to execute the trade-in of AB Sciex 4500 QTrap. No additional costs shall be incurred by CBP as the vendor shall cover all shipping costs associated with the return shipment of the AB Sciex 4500 QTrap.

Delivery:

Delivery shall be coordinated with the New York Laboratory to ensure that someone is present to accept the delivery. Delivery of equipment must occur between 9:00 AM and 4:00 PM Local Time (to accommodate loading dock availability). Delivery must take place at street level (liftgate delivery), as

5 | P a g e no elevated loading dock is available.

US Customs and Border Protection New York Laboratory

1100 Raymond Blvd., Suite 550

Newark, NJ 07102

Contractor shall provide copies of the documents listed below. One copy of the data shall be delivered in paper form and one copy in electronic form. The electronic form shall be in MS Word format or

Adobe Acrobat PDF. Point of contact information to be provided upon award.

Any associated software license/key documentation and (or) software download link(s).

The document(s) are deliverables under the contract and shall be delivered with equipment delivery and/or no later than thirty (30) days after shipping date.