Attachment_1_SON_CytoFLEXUpgrade.pdf

STATEMENT OF NEED (SON)

1.0 TITLE

CytoFLEX S Flow Analyzer Upgrade from V2-B2-Y2-R0 to V2-B2-Y3-R2.

2.0 BACKGROUND

The LCMB studies Wnt signaling, which is an important signaling pathway involved in the development of tissue homeostasis and cancer. The output of the Wnt pathway is the transcriptional activation of target genes that execute diverse cellular programs. The main readout of Wnt pathway activity is used in the lab as a reporter system in which the fluorescent protein GFP is driven by a Wnt-responsive element. LCMB also uses fluorescent protein fusions and immunofluorescent staining to measure changes in Wnt pathway components. Therefore, the LCMB uses a CytoFLEX S Flow Analyzer to perform flow analysis on a daily basis to measure the effects of genetic or pharmacological perturbations on the Wnt pathway. Their current instrument uses three lasers – violet, blue and yellow/green – and their associated detectors to measure these parameters. However, LCMB’s emerging need is to be able to measure 3-4 fluorescent signals simultaneously, which requires a red laser and additional detectors to be added to the labs CytoFLEX S Flow Analyzer.

3.0 TYPE OF ORDER

This is a Firm Fixed-Price Purchase Order.

4.0 SPECIAL ORDER REQUIREMENTS

4.1 PRODUCT FEATURES/SALIENT CHARACTERISTICS

The following product features/characteristics are required for this requirement:

One (1) USB Dongle (Product No.:C04721) for CytoFLEX S Flow Analyzer Upgrade from a V2-B2-Y2-R0 to V2-B2-Y3-R2 configuration. Configuration of the instrument after upgrade shall be as follows:

Laser Wavelength Power #Detectors Violet 405 nm 80 mW 2 Blue 488 nm 50 mW 2 Yellow Green 561 nm 30 mW 3 Red 638 nm 50 mW 2

Configuration adds one (1) Red Laser and 3 additional detectors.

Parts must be fully compatible with the pre-existing Beckman Coulter CytoFLEX S Flow Analyzer (Serial No.:BD13108).

Upgraded must enable the system to measure 3-4 fluorescent signals simultaneously.

STATEMENT OF NEED (SON)

4.2 DELIVERY / INSTALLATION

Delivery and installation of specified items shall be within forty-five (45) days of award. All shipping/handling shall be F.O.B Destination, and pricing shall be included in the quote. The items shall be delivered to the Technical Point of Contact (TPOC), TBD at award, at the following address:

Laboratory of Cellular and Molecular Biology 9000 Rockville Pike Bldg.37, Room 2056 Bethesda, Maryland 20892

Upgrade shall be installed on the Government owned Beckman Coulter CytoFLEX S Flow Analyzer (Serial No.: BD13108). Installation of the required components must be performed by OEM trained and certified personnel. Upon completion the contractor will verify system is fully operational and scientifically accurate.

5.0 WARRANTY

In addition to the implied warranty contained in FAR Clause 52.212-4, quoters are encouraged to provide express warranties. At a minimum, the Contractor shall provide a 12-month warranty or longer from date of final installation on all equipment and associated items provided under, or as a part of this purchase order. The warranty shall include repair and or replacement of parts, including travel, labor, and materials, at no additional cost to the Government. The warranty shall not exclude or limit the Governments rights for an implied warranty as established in the terms and conditions of clause 52.212-4.

6.0 TECHNICAL POINT OF CONTACT (TPOC)

The Technical Point of Contact/s (TPOC) is responsible for inspection and acceptance of all work in relation to this requirement and will serve as the Governments primary point of contact throughout the duration of the requirement. All efforts must be coordinated with this individual/s. The TPOC for this requirement is:

TBD (upon award)

Note: The TPOC has limited authority as defined within this Statement of Need. They do not have the authority to commit or obligate Government funds or make changes to the terms and conditions of the purchase order. As such, any changes to the purchase order must be performed by a warranted Government Contracting Officer with appropriate delegated authority to do so.