Attachment 1 - Salient Characteristics.pdf

Compact Benchtop Mass Spectrometer (CMS) | Technical Specifications

Salient Characteristics & Requirement Specifications

Project: Compact Benchtop Mass Spectrometer (CMS)

Document Type Technical Specification & Requirements

Scope Procurement of research-grade compact mass spectrometer & accessories

Table of Contents Table of Contents

1. General System Description

2. Core Instrument Specifications

3. Control, Integration, and Data Processing Software

4. System Accessories and Interface Upgrades

5. Ancillary Hardware, Installation, and Service Support

1. General System Description The system must be a compact, benchtop single quadrupole mass spectrometer with a liquid introduction interface and manual flow injection valve, designed for rapid, real-time chemical and molecular analysis directly at a chemist's bench. The instrument must support versatile ionization techniques (ESI and APCI) and interface seamlessly with existing laboratory liquid chromatography infrastructure.

2. Core Instrument Specifications The instrument must meet or exceed the following core hardware and performance specifications:

Parameter Requirement Specification

Ion Sources Must include both Electrospray Ionization (ESI) and Atmospheric Pressure Chemical Ionization (APCI) ion sources.

Sample Introduction Must have direct injection operation and include a manual, dual-position rotary liquid injection valve installed directly inside the spectrometer frame/chassis.

Mass Analyzer Must be a single quadrupole mass filter covering a mass-to-charge (m/z) range of at least m/z 10 to m/z 2,000.

Flow Rate Range Must operate reliably across a flow rate range of 10 μL/min to 1,000 μL/min.

Polarity Switching Must be capable of performing positive and negative ion analyses in a single analytical run.

Acquisition Rate Must support high-speed scanning up to 10,000 m/z units/sec, ensuring compatibility with UPLC and UHPLC systems.

Resolution Must resolve peaks at 0.5 to 0.7 m/z units (Full Width at Half Maximum – FWHM) at a scan speed of 1,000 m/z units/sec over the entire mass range.

System Sensitivity Must detect 100 pg of reserpine injected on-column with a minimum Signal-to- Noise (S/N) ratio of 100:1 (full-scan acquisition from m/z 100 to m/z 1,200).

Must detect 10 pg of reserpine injected on-column with a minimum S/N ratio of 100:1 using Selected Ion Monitoring (SIM) of m/z 609.28.

Mass Accuracy Must be within ± 0.1 m/z units over the entire acquisition range.

Mass Stability Must vary by no more than ± 0.1 m/z units over a continuous 30-day operating period.

Physical Dimensions Must be a compact benchtop unit designed to fit inside a standard laboratory fume hood, with physical dimensions no larger than 11 inches W x 22 inches D x 26 inches H.

Parameter Requirement Specification

Electrical Requirements Must operate from a standard North American 110V, 15Amp electrical outlet.

Nitrogen Gas Consumption Must require no more than 60 PSI of N2 gas pressure for full operation.

3. Control, Integration, and Data Processing Software The system software suite must provide comprehensive instrument control, advanced data processing, and flexible integration capabilities as detailed below:

• Multi-System Instrument Control: System must include software capable of controlling both the mass spectrometer and the existing High-Performance Liquid Chromatography (HPLC) system from a single software interface.

• Background-Subtraction Peak Detection: Software must include an automated peak detection utility capable of filtering out chemical background noise to automatically identify small peaks without prior knowledge of the target m/z ratios.

• Cross-Vendor Software Integration: Must support integration with standard open-source or cross-vendor instrument control frameworks to facilitate third-party hardware integration.

• Deconvolution Utilities: Software must include both automated and manual charge deconvolution capabilities for interpreting multiply charged species.

• Analog/Digital Outputs: Must feature programmable analog outputs providing up to 4 simultaneous, real-time Extracted Ion Chromatogram (XIC) and Total Ion Chromatogram (TIC) channels.

• External Input/Output (I/O): Must support connection of up to 16 digital and analog I/O ports at the front and back of the instrument to facilitate custom chemistry workflow configurations.

4. System Accessories and Interface Upgrades To support diverse research workflows and future scalability, the system must offer the following optional or scalable interface kits:

• Planar Surface Analysis Interface: Must feature an option for a Thin-Layer Chromatography (TLC) plate reader or direct analysis interface that couples directly to the mass spectrometer for solvent-free analysis of planar surfaces, paper, or tissue samples.

• Direct Solids/Liquids Probe: Must feature an option for a direct, solvent-free solids and liquids analysis probe (such as an Atmospheric Solids Analysis Probe or equivalent).

• Headspace and Gas Analyzer: Must feature an option for direct identification of volatile/semi-volatile compounds in air, headspace, breath, or gas samples without requiring manual sample preparation.

• Continuous Flow Sampling: Must feature an option for a continuous-flow open port sampling interface or surface sampling system.

• Preparative and Liquid Chromatography Interfaces: Must feature options/kits for direct interface coupling to Flash Chromatography, Supercritical Fluid Chromatography (SFC), and Preparative HPLC systems.

5. Ancillary Hardware, Installation, and Service Support The procurement must include all necessary ancillary hardware, software licenses, and professional services required for a complete, turn-key installation:

• Vacuum Pumping System: Must include a rotary vane vacuum pump equipped with an oil mist filter and oil return mechanism, compatible with standard 110V electrical outlets.

• Exhaust System: Must include a diaphragm pump-based ion source exhaust kit and exhaust trap to allow safe laboratory venting.

• Dedicated Workstation: Must include a dedicated compatible laptop/computer pre-configured with all system control, acquisition, and data analysis software.

• Warranty: Must include a minimum of a 12-month hardware and software warranty upon completion of installation.

• Installation and Training: Must include professional, on-site installation and basic operator training covering instrument startup, sample introduction, routine maintenance, and software operation.