Attachment 1 - Purchase Description.pdf

Version 11-30-2016-1 FAR Part 11—Describing Agency Needs

PURCHASE DESCRIPTION

Brand-name or Equal: LightSpeed Mode Upgrade for Existing Miltenyi Biotec

UltraMicroscope Blaze System

1. Statement of Need and Purpose:

The purpose of this request is to purchase a manufacturer-recommended LightSpeed Mode hardware and software upgrade to the existing Miltenyi Biotec light sheet UltraMicroscope Blaze imaging system that would best meet the current and future Facility needs for 3D multispectral imaging applications of whole organ tissues, including whole mouse, rat and monkey brains, and large specimens of human brain tissue, after tissue clearing. This hardware and software upgrade includes the replacement of required electronic and optical parts in addition to the installation of new LightSpeed image acquisition and MACS iQ View 3D image processing software, and the acquisition of a custom configured scientific computer seamlessly integrated to control the upgraded functionalities of the UltraMicroscope Blaze imaging system, all of which are only available from Mitenyi Biotec. This upgrade will enable up to a 60-fold increase in imaging speed and continuous movement of the scanning stage with optimal camera synchronization to enable fast tiling 3D scans at high spatial resolution.

2. Background Information and Objective:

FCICF supports basic and clinical research within NINDS and across NIH and has been receiving increasing demand for multispectral 3D light-sheet scanning of wide variety of cleared tissue samples, which currently take from several hours to several days to scan per sample, depending on sample size, number of imaging channels, and imaging resolution acquired. The aforementioned light-sheet hardware and software upgrade will cut down the scanning time of these samples by up to 60-fold and thereby improve the bandwidth and productivity for supporting light-sheet imaging projects at this Facility.

3. Generic Name of Product:

LightSpeed Mode Upgrade to Existing Miltenyi Biotec UltraMicroscope Blaze

4. Purchase Description:

Table A:

Description Quantity LightSpeed mode Upgrade package for UltraMicroscope Blaze™.

The upgrade package contains:

- 1x Blaze PC

- 1x Lightspeed license

- Blaze electronics required for the upgrade

One (1)

UMB Blaze Slider holder with clamping 3D printed Slider holder for UltraMicroscope Blaze. Allows fitting of several specimen sizes via movable slider. With clamping system to

One (1) ensure tight adhesion to the stage arm. Chemically smoothed following 3D print. The package includes an additional spare slider.

UMB Sample holder base 19mm w. clamping 3D printed sample holder base 21 mm for UltraMicroscope Blaze. With clamping system to ensure tight adhesion to the stage arm. Chemically smoothed following 3D print.

One (1)

UMB Sample holder base 60mm w. clamping 3D printed sample holder base 52 mm for UltraMicroscope Blaze. With clamping system to ensure tight adhesion to the stage arm. Chemically smoothed following 3D print.

One (1)

UMB LightSpeed mode Upgrade service UMB LightSpeed mode Upgrade service. This service covers the costs of installing the 130-133-683 UMB Lightspeed mode Upgrade package for the Ultramicroscope Blaze.

The following is included in this service:

-all travel cost -all labor cost including: Installation of new PC, exchange motor control board, driver installation, functional control

One (1)

UMB knife-edge calibration tool Knife-edge tool for light sheet calibration of the UltraMicroscope Blaze.

Related to deconvolution in MACS iQ View - 3D Large Volume package.

One (1)

MACS iQ View 3D pkg Lic Perm Comm Software MACS iQ View – 3D Large Volume package. Permanent license commercial.

Includes 2 years of software updates. Image processing software for UltraMicroscope Blaze data. Includes: 3D cropping, destriping, denoising, deconvolution, stitching, contrast compression, and batch processing.

One (1)

5. Salient Characteristics:

The system must meet the following physical, and performance, functional characteristics:

I. Full compatibility of optical and electronic components with existing UltraMicroscope Blaze imaging system.

II. Full software compatibility for fast 3D multispectral image acquisition and post-acquisition big image data processing using the UltraMicroscope Blaze imaging system.

III. Up to 60-fold increase in acquisition speed performance for fast 3D multispectral imaging of large tissue specimens, including whole mouse, rat and monkey brains, and large specimens of human brain tissue, after tissue clearing.

IV. Custom slide holders with clamping to mount tissue specimens up to 60 mm in size.

V. Knife-edge calibration tool for light sheet calibration of the UltraMicroscope Blaze imaging system.

VI. A custom-configured scientific imaging computer fully compatible to seamlessly support the fast tile 3D big image data acquisition using the UltraMicroscope Blaze imaging system enabled by LightSpeed acquisition imaging hardware and software upgrade.

VII. Permanent license of MACS iQ View 3D imaging software for post-acquisition processing of extremely large image datasets ranging in the multi-TB image size range. The software must include seamless functionalities for 3D cropping, de-striping, denoising, deconvolution, image stitching, contrast compression, and batch processing.

6. Quantity: As stated in Table A (above).

7. Delivery and Installation Requirements: All components of the identified in the “Purchase Description” section above shall be delivered and installed within 30 days after receipt of order. Delivery shall be FOB Destination to the address below, between the hours of 8:30 am and 5:00 pm Eastern Time, Monday through Friday (except on Federal holidays). Installation must be performed by Miltenyi Biotec-authorized and certified personnel.

Attention: Dragan Maric Ph.D.

National Institutes of Health Flow & Imaging Cytometry Core 10 Center Drive Bldg. 10, Rm. 5S-241 Bethesda MD 20892

USA

8. Warranty: The Contractor shall warrant that all non-software items described in the “Purchase Description” section will be free from defects for a period of at least one (1) year after the date of installation, inspection, and acceptance by the Government. The Contractor shall further warrant that all software described in the “Purchase Description” section will be from defects for a period of at least two (2) years from the date of installation, inspection and acceptance by the Government. The Contractor shall also provide all OEM-recommended software updates for a period of at least two (2) years after the date of activation of the software. This Warranty shall include troubleshooting services based on complete knowledge of the entire UltraMicroscope Blaze imaging system.

PURCHASE DESCRIPTION