Attachment 1 Purchase Description.pdf

PURCHASE DESCRIPTION

1. Statement of Need and Purpose: The CBBU at NINDS would like to acquire an XYZ Stage with Heidenhain encoders and 300µm travel z-piezo plate and required cables and connectors. This microscope stage will allow us to scan samples in three dimensions with extremely high repeatability. This is necessary in order to image samples that are created via expansion microscopy or to image cells over long periods of time. This stage has precision XY encoders that are tuned to the proper stage speed necessary for imaging expansion microscopy samples and will enable many new avenues of exploration.

2. Background Information and Objective: The CBBU at NINDS investigates microtubules in vitro and in vivo at spatial scales ranging from nm to µm. We have a long history of exploring the structure and function of tubulin and microtubules as well as microtubules associated proteins (MAPs) in vitro. We are now moving toward in vivo imaging and expansion microscopy. Microtubules in cells can be bundled together and via expansion microscopy we hope to resolve these bundles. In general a sample that is expanded 10 x can be up to 300µm in size so this stage is also equipped with a piezo z-top that will allow high precision repeatability measurement along the optical axis of the microscope.

3. Generic Name of Product:

XYZ Stage

4. Salient characteristics:

Vendor shall provide the following minimum salient characteristics:

o Heidenhain XY Linear encoders o Piezo Top plate with 300 µm range of travel and 4.2nm minimum step size o Stage for a Nikon Ti-2 Microscope with Stage up Kit o Two axis DC servo motor control card for piezo o required cables and connectors

5. Quantity:

1 XYZ Microscope Stage

6. Delivery Requirements:

The contractor shall deliver the equipment within 160 days after receipt of the order. The

Site location address is Building 35, Room 3B-205, 35 Convent Drive, Bethesda, MD 20892-3706.

7. Warranty Requirements:

Contractor shall warrant that the Equipment will be free from material defects for a period of twelve (12) months from the date of acceptance. All service performed on this equipment shall be provided by OEM certified and factory trained technicians.

Technicians shall have the ability to diagnose the systems on site via remote access.

Contractor shall provide any required replacement components due to malfunctions on-site. Warranty shall not include periodic maintenance services.