Attachment 1 - Purchase Description.pdf

Version 11-30-2016-1

FAR Part 11—Describing Agency Needs

PURCHASE DESCRIPTION

1. Statement of Need and Purpose: The cortex-cerebellum circuitry lab needs fixed wavelength, femtosecond pulsed lasers at both 920 nm and 1064 nm to perform multi -site and multi-color in vivo two photon brain imaging and two photon optogenetics between the cortex and cerebellum. Specifically, a high-power femtosecond 920 nm laser is critical to powering a dual-site imaging strategy for green calcium indicators (like

GCaMP) while a high-power femtosecond 1064 nm laser is critical to simultaneously powering a dual-site imaging strategy using red indicators (like RCaMP) as well as activating two-photon optogenetic proteins.

2. Background Information and Objective: A major goal of the research performed by the lab is to understand communication between the neocortex and cerebellum while animals learn novel behavioral skills over weeks. The lab measures activity in both cortex and cerebellum simultaneously using a custom dual-site two-photon microscope, across multiple genetically defined populations using dual-wavelength two-color imaging, and by combining imaging of one neural population with stimulation of another. An essential component of this is fixed wavelength femtosecond lasers at 920 nm and 1064 nm with >1.5 W of power output per channel.

3. Generic Name of Product: Dual output femto-second pulsed fiber lasers with dispersion precompensation.

4. Product Description:

This requirement is issued on a brand name or equal basis to:

Spark Lasers Alcor 920nm + 1064nm dual output 2W fiber laser

5. Salient characteristics

• Wavelengths: 920 nm and 1064 nm, separate outputs

• Average power: 2,000 mW per wavelength output

• Pulse duration (fs): 100

• Repetition rate: 80 MHz

• Beam diameter: 1.4 ±0.2 mm for the 920 nm beam; 1.5 ±0.2 mm for the 1064 nm beam

• Power stability (%): 1

• Dispersion precompensation (fs2): 0 to - 60,000

• Control interface: RS-232 or USB

6. Quantity: Two (2)

Version 11-30-2016-1

FAR Part 11—Describing Agency Needs

7. Delivery Date: The Contractor shall deliver and install the equipment described above as soon as available but no later than 8-12 weeks after receipt of order. The Equipment shall be delivered to the National Institutes of Health, 49 Convent Drive, Building 49, Room 2A-35 Bethesda, Maryland 20892 between the hours of 8:00 am and 5:00 pm, Eastern Daylight Time (EDT), Monday through Friday. Delivery shall be freight on board

(FOB) destination and include inside delivery.

8. Warranty Requirements: 2 year mfr warranty.

9. Option(s): N/A