Attachment 1 - Purchase Description.pdf

Version 11-30-2016-1 FAR Part 11—Describing Agency Needs

PURCHASE DESCRIPTION

1. Statement of Need and Purpose: Projects being pursued by Behavioral Neuroscience (SBN) require the acquisition of a ready to use (turnkey) wireless fiber photometry (FP) system. Fiber photometry allows measuring of neural activity via calcium and neurotransmitter-specific sensors. There are two wired FP systems already in use at SBN and two more wireless FP systems will complement the existing FP systems as well as expand SBN’s members ability of recording neuronal activity in a larger set of behavioral tests. Wireless FP system is especially suitable for work with non-human primates.

2. Background Information and Objective: The Section on Behavioral Neuroscience at the National Institute of Mental Health (NIMH) is focusing on current approaches into investigations of brain circuits and systems that require detailed analyses of neuronal circuit dynamics in freely moving animals with neurotransmitter specificity. Fiber photometry is a neuronal imaging technique used to monitor neuronal activity of specifically targeted cell populations. Fiber photometry uses excitation light from implanted fiber optics to record fluorescent activity of genetically encoded calcium or neurotransmitter-specific indicators in neuronal populations that fluoresce in the presence of calcium ions or neurotransmitter molecules. This imagining can be used at the same time that behavioral or electrophysiology experiments are being conducted giving a better understanding of the underpinning of brain function and its role in animal behavior in freely moving animals. Wireless approaches to data recording allow animals freedom of movement and are easier to combine with other testing equipment.

3. Generic Name of Product: Wireless Fiber Photometry System

4. Purchase Description:

TeleFiOpto-set (standard configuration) Product Code:E60.000.00

Includes: 1x TeleFiOpto Headstage, 1x TeleFi Charger, 1x TeleFi Cannula, 1x TeleFi Insertion Tool, 1x TeleFi Dummy Headstage, 1x TeleOpto Remote Controller, 1x Infrared Emitter, 1x Stimulator for Optogenetics.

TeleFiOpto-set (dual position configuration) Product Code: E60.000.00 Includes: 1x TeleFiOpto Headstage, 1x TeleFi Charger, 1x TeleFi Cannula, 1x TeleFi Insertion Tool, 1x TeleFi Dummy Headstage, 1x TeleOpto Remote Controller, 1x Infrared Emitter, 1x Stimulator for Optogenetics.

Version 11-30-2016-1 FAR Part 11—Describing Agency Needs

5. Salient Characteristics:

-This wireless fiber photometry system is a small size and has a wireless signal transmission distance that is 2 m in standard configuration and up to 5 m with an additional antenna. The wireless transmission means there are no cords to impede animal’s movement and also introduce artifacts in the recorded signal.

-The two new systems will complement two existing wired FP systems but allow neuronal data recording in experimental settings where animals are jumping and moving in the vertical direction, as non-human primates tend to do. In such conditions a wireless FP is needed to avoid large movement-related artifacts in the recorded data and to prevent damage of the FP equipment.

-Wireless transmission means that there are no cords that impede animal’s movement and also introduce movement artifacts in the recorded signal.

-Can be used for both rats and marmosets in the lab and contains a small headstage good for mice, rats, marmosets, etc.

6. Quantity: Two (2) TeleFiOpto-Sets (Standard Configuration) and Two (2) TeleFiOpto-

Sets (dual position configuration)

7. Delivery Date: 30 days after receipt of order

Delivery Address:

National Institute of Mental Health 35A Convent Drive Room: 2D-804A Bethesda, MD 20892 301-496-8815

8. Period of Performance: One year warranty period included with purchase.

9. Option(s): N/A