Attachment 1 Purchase Description CSS FINAL.pdf

PURCHASE DESCRIPTION

1. Statement of Need and Purpose: The Neurosurgical Oncology Unit uses several molecular biology and biochemistry techniques in our scientific mission to better understand pathogenesis of gliomas and develop better therapies. While we perform several assays such as cell cycle, a technology that provides rapid multi-parametric analysis of cells in soluti, etc. We do not currently have instruments within our group to efficiently and reliably do this work. The ultracentrifuge will allow us to perform several assays in a single unit and will expand the repertoire of biochemical and molecular biology techniques our group will be able to perform.

The procurement of the Beckman Optima XPN-90 Ultracentrifuge will provide intra-laboratory high content ultracentrifuge for our laboratory that will be compatible with current research protocols within the NIH and compatibility with data and software on the current ultracentrifuge research facility which provides basic research support to members as well as to investigators throughout NIH Support is provided using instruments manufactured by Beckman. Our laboratory requires this same type of instrument for seamless operation and data acquisition.

The research projects supported are complex and varied. As such, the laboratory is required to maintain equipment that provides for a wide range of applications and that includes the ability to modify and adapt capabilities as new applications are developed.

2. Project Background: Glioblastomas are the most common malignant CNS neoplasm. Despite aggressive surgery, chemotherapy and brain radiation, median survival is only 15 months and the disease remains uniformly fatal. The NOU performs mission critical research on the molecular and cellular biology unique to glioblastomas with an expressed goal of developing novel therapeutic strategies to help patients. We recently used a publicly available genetic glioma dataset to probe a novel hypothesis regarding how primary cilia may affect glioma signal transduction and cancer phenotype. The data was confirmed using a separate, unrelated, publicly dataset. The goal of this purchase through GeoMx is to use tissues from our patients available in the biobank within the Surgical Neurology Branch (SNB) to confirm that our findings are indeed relevant to the patient population here at NIH whose lives we seek to improve. This is a necessary and important step in order to publish our findings in a high-impact journal. Additionally, the information gained will allow us to leverage our limited resources to determine and develop the targets with highest success potential. The Neurosurgical Oncology Unit uses several molecular biology and biochemistry techniques in our scientific mission to better understand pathogenesis of gliomas and develop better therapies. While we perform several assays such as cell cycle, a technology that provides rapid multi-parametric analysis of single cells in solution , etc., we do not currently have instruments within our group to efficiently and reliably do this work. The Ultracentrifuge will allow us to perform several assays in a single unit and will expand the repertoire of biochemical and molecular biology techniques our group will be able to perform.

3. Generic Name of Product: Ultracentrifuge

4. Purchase Description:

• Qty 1, Optima XPN 90 – IVD Ultra Centrifuge

• Qty 1,Type 70,1Ti Fixed angle rotor, 12x 13.5 mL, 70,000 rpm 450,000 X g

• Qty. 3, 10.4 mL, Polycarbonate, Bottle and Cap Assembly (pack of 6) (GSA# 355603)

• Qty 1, Optima XPN 80 installation service

Trade Name: Beckman Optima XPN90

Products brand name: Beckman Coulter Optima XPN-90

Manufacturers name: Beckman Coulter

5. Salient Characteristics Our laboratory is in need of an ultracentrifuge with user-friendly, advanced intelligence software and large touch-screen display that is easy to operate and effortlessly manages security and electronic records, increasing productivity and ensuring confidence. In addition, it is imperative that we obtain a reliable, state-of-the-art ultracentrifuge with powerful simulation tools and networking capabilities to meet our present and future needs. The ultracentrifuge will help us perform our current separations including extra cellular vesicles and develop new procedures with its innovative, intuitive software. We are unable to find an instrument from another vendor with these necessary features.

• Must have 15” LCD display with ± 45° horizontal and vertical tilt for easy operation (even with gloved hands) and viewing from virtually anywhere in the lab.

Must have unique Beckman SW32Ti Rotor Package.

• The drive must be covered by a 10-year warranty.

• Must have heating/cooling system that does not use compressors or chlorofluorocarbons (CFCs). The thermoelectric heating/cooling unit provides fast chamber/rotor refrigeration or heating and does not require any compressors, reducing instrument down time and heat and noise levels.

• Must have an air-cooled direct drive system that requires no gears, brushes, oil vacuum seal, external oil reservoir, or continuously-operating damper. This provides efficient operation and extends the life of the instrument. Air cooling eliminates chlorofluorocarbons and compressors which results in less instru-ment down time and less output of heat and noise.

• Must operate at a noise level below 51 dBa.

• Must achieve vacuum level below 5 microns (0.7 Pa).

• Must consume no more than 60W of power while idling (powered on, but not running).

• Must have available Near Vertical Tube (NVT) rotors to maximize lipoprotein and plasmid DNA separa-tions for speed and purity.

• Must be compatible with all currently-produced Beckman Coulter floor preparative ultracentrifuge rotors.

• Must have tube options including: OptiSeal (for easy sealing without tools), Quick-Seal (fast heat-sealing), and g-Max (shortens separation times by up to 50 percent) and Ultra Clear Tubes.

• Must be designed and tested as a system with Beckman Coulter rotors, tubes and accessories.

• The Contractor shall provide an Original Equipment Manufacturer (OEM) certified technician to perform the setup and installation of the equipment.

SOFTWARE

• Must have an interactive touchscreen interface with eXPert operating software (without the need for an external computer), which includes:

• Software available in local language for easy, error-free operation, with the option to select other languages including: English, Spanish, French, German, Italian, Chinese (simplified), Japanese, Korean, and Russian.

• On-screen, context-sensitive help.

• User log-in function with up to 50 unique users and passwords.

• Rotor logging by serial number to enable tracking of rotor usage.

• Run logs for record-keeping or lab procedures purposes via user log-in functionality.

• Electronic signature and run comment capabilities.

• Up to 1,000 user-defined programs capable of 30-step programming.

• Multiple acceleration and deceleration profiles. Flexibility in acceleration and deceleration are essential if procedures are to be done correctly and reproducibly. Up to 10 acceleration and 11 deceleration rates are available for any run.

• Export run data in .xml and .csv format to Microsoft Excel or other spreadsheet formats through a standard USB port to a flash drive.

• Setting up a run by specifying RCF (relative centrifugal force), ω2t (accumulated centrifugal ef- fect), substitute rotor and tube combination, or sedimentation coefficient

Additional Salient Characteristics:

• Instrument specifications must be kept at ambient temperatures of up to 35°C

• The ultracentrifuge system must have two independent safety systems including a rotor inertia check to avoid over speeding of rotors

• Instrument must have regenerative breaking

• Instrument must be tolerant to power fluctuations

• Instrument must have a switching power supply to maintain the control of the instrument´s system

(without the drive) in case of power sags, at east down to 100VAC

• The user interface must provide the option to add pre- and post-run comments that will be included in the run report

• The user interface must provide the option to directly transfer calculated run parameters to the instrument control

• The user interface must provide the option for electronic signatures

• The ultracentrifuge must have an Application Programmable interface, API

• Labware: Supplier must offer ready to use sterile labware

• Labware: Supplier must offer labware that is certified to be free of endotoxins, DNAses, RNAses and

PCR inhibitors.

• Software: The method transfer and application development software must include k-factors for all available labware options

6. Quantity: 1

7. Delivery Date: 30 days ARO. Please specify in quote the earliest time frame for delivery after award.

8. Warranty: The Contractor shall warrant that all computer equipment (computer, monitor) will be free from defects for a period of at least three (3) years from the date of installation, inspection by the Government, and acceptance. The Contractor shall further warrant that all electronic, optical and mechanical components will be free from defects for a period of at least five (5) years from the date of installation, inspection and acceptance by the Government. Warranty service shall include trouble-shooting capabilities based on complete knowledge of the entire system. Warranty shall not include routine or periodic maintenance services.

9. Options(s): Provide pricing for extended additional service agreements for a period of up to three years that may be included in the award.