Attachment 1 - MECs - Shank 2 Cryorecovery Support Services.pdf
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- Attached to
- Shank 2 Cryorecovery Support Services for the Medical Readiness System Biology (MRSB) at WRAIR
- Solicitation number
- PANMRA24P0000-004380
- Issued by
-
Defense Health Agency
About this file
This document is a Minimum Essential Characteristics (MEC) requirement for a federal contract opportunity titled "Shank 2 Cryorecovery Support Services for the Medical Readiness System Biology (MRSB) at WRAIR".
The key requirements are for the contractor to develop a mouse colony from cryopreserved sperm of 2 male Shank2 homozygous Nestin-Cre hemizygous mice provided by WRAIR. The contractor must provide WRAIR with two shipments of 10 and then 30 male mice of this genetic line at 6-8 weeks of age. The contractor must also provide protocols for tamoxifen injection, gene editing testing, and maintaining the mouse colony. Additionally, the contractor must isolate and ship cryopreserved sperm from the 2 male mice to WRAIR for long-term storage.
This is a pre-solicitation notice, not a request for proposals or quotes. Responses will not be considered as offers, and no contract will be awarded. The government is only seeking information on contractor capabilities to support this requirement.
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Text version
Minimum Essential Characteristics (MEC)
Requirement Title
Contractor will develop a mouse colony from cryopreserved sperm from 2 male
Shank2 homozygous Nestin-Cre hemizygous mice provided by WRAIR.
Supply WRAIR with the first shipment of 10 male Shank2 homozygous Nestin-
Cre hemizygous mice at 6-8 weeks of age.
After a minimum of two months after delivering shipment one, supply WRAIR with a second shipment of 30 male Shank2 homozygous Nestin-Cre hemizygous mice at 6-8 weeks of age.
Provide a protocol about how to inject tamoxifen (dose etc.) to mice for inducible-
CRE activation.
Deliver the protocol to test the success of gene editing, e.g. PCR protocol, primer and kit information
Provide a protocol outlining the methodology necessary for developing and maintaining a mouse colony.
Isolated and ship cryopreserved sperm from 2 male Shank2 homozygous Nestin-
Cre hemizygous mice to WRAIR for prolonged cryopreservation.
File details come from the government source that posted it. Updated .