Attachment 1 FINAL Purchase Description.pdf

Version 11-30-2016-1 FAR Part 11—Describing Agency Needs

PURCHASE DESCRIPTION

1. Statement of Need and Purpose: Acquisition of one (1x) Nikon Ts2-Fl Inverted Microscope System w/ DS10 Camera to allow for better observation of mammalian cells and model organisms such as C. elegans via Brightfield, Phase Contrast, Emboss Contrast, and Fluorescence. This will support our increased efforts in cell line development as the system has increased quality and clarity of view. The system will also allow for imaging of cells within dishes and flasks during their growth stages, including cells with fluorescently-labeled proteins. This will decrease the chances of cell culture contamination as we will no longer need to transport flasks outside of the cell culture room for observation and imaging of fluorescence.

2. Background Information and Objective: The National Institutes of Health (NIH) is the nation’s leading medical research agency and the primary Federal agency whose mission is to seek fundamental knowledge about the nature and behavior of living systems and the application of that knowledge to enhance health, lengthen life, and reduce illness and disability, conducting, supporting and making medical discoveries that improve people’s health and save lives.

The National Center for Advancing Translational Sciences (NCATS) is a part of the National Institutes of Health (NIH), which mission it is to catalyze the generation of innovative methods and technologies that will enhance the development, testing and implementation of diagnostics and therapeutics across a wide range of human diseases and conditions.

NCATS is tasked with identifying and developing novel small molecule, peptide and biologic leads against a wide spectrum of human disease targets, which it accomplishes by screening thousands of compounds in collaboration with both intra-and extramural researchers. In pursuit of this goal, the Center has a strong internal transdisciplinary team of scientists who continually develop and optimize biological assays and discover novel small molecules for application against a wide range of human diseases.

The Assay Development and Screening Technologies (ADST) laboratory focuses on the advancement of therapeutic development through research and implementation of innovative assay designs and chemical library screening methods related to disease biology. Towards this effort there is a large emphasis on mammalian cell culture and model organism, including C. elegans, assays to model human pathophysiologies. These systems require continuous maintenance and visual validation of cell/organism health and proliferation for both assay development and high-throughput screening efforts. As such it is necessary to have fully operational and equipment cell culture and nematode culture areas for the growth and use of these model systems.

3. Generic Name of Product: Inverted Microscope System

4. Purchase Description: Nikon Ts2-Fl Inverted Microscope System w/ DS10 Camera. Includes the Eclipse Ts2-FL Inverted Fluorescence Microscope, Epi Fluorescence LED and Filter Sets, Nikon DS10 Color Digital Camera Head, and NIS-Elements, Documentation [D] (Multi License) software

5. Salient characteristics: The (1x) Nikon Ts2-Fl Inverted Microscope System w/ DS10 Camera will allow for better observation of mammalian cells and model organisms such as C. elegans via Brightfield, Phase Contrast, Emboss Contrast, and Fluorescence. This will support increased efforts in cell line development as the system has increased quality and clarity of view. The system will also allow for imaging of cells within dishes and flasks during their growth stages, including cells with fluorescently-labeled proteins.

This will decrease the chances of cell culture contamination as we will no longer need to transport flasks outside of the cell culture room for observation and imaging of fluorescence.

The system will include:

Eclipse Ts2-FL Inverted Fluorescence Microscope Epi Fluorescence LED and Filter Sets Nikon DS10 Color Digital Camera Head NIS-Elements Documentation [D] (Multi License) Software

Detailed Specifications to include the following:

1. Eclipse Ts2-FL Inverted Fluorescence Microscope High Luminescent LED Illuminator (60K+hrs) w/ Fly-Eye Lens 100v-240v ELWD Condenser (na O.3) W.D.75mm, 5-Place Nosepiesce Plain Stage with Acrylic Insert, & Dust Cover

i. 2 MMK30105 - CFI Widefield Eyepiece 10x / 22 (TS2W)

ii. 1 MFV51000 - Light Shield (Blocks Ambient Light) (TS2LS)

iii. 1 92080 - Eclipse Microscope Pad

iv. 1 79035 - A.C. Power Cord BU [100-120v]

v. 1 MFB36100 - Camera Port 100 Eye/100 Camera (TS2PCF) (ISO)

vi. 1 MFV30010 - Phase Slider PhL, Ph1, BF (T1SNCP)

vii. 1 MRP00042 - CFI Achro Flat Field 4x / 0.10 / 30.0

viii. 1 MRP40102 - CFI Achro ADL 10x / 0.25 / 6.20 Phase 1

ix. 1 MRP46202 - CFI LWD Achro ADL 20x / 0.40 / 3.10 Phase 1

x. 1 MRP46402 - CFI LWD Achro ADL 40x / 0.55 / 2.10 Phase 1

b. Epi Fluorescence LED and Filter Sets

i. 1 96240 - C-FL LED-385nm Ex390/38, Dm420, Em475/90 (DAPI)

ii. 1 MBF84715 - 385nm LED Light Engine

iii. 1 96242 - C-FL LED-470nm Ex470/40, Dm500, Em534/55 (FITC / GFP)

iv. 1 MBF84735 - 470nm LED Light Engine

v. 1 96231 * - AT Sputter Ex560/40, Dm600, Em635/60 (TxRd, mCherry, Alex594)

vi. 1 MBF84785 - 560nm LED Light Engine

c. Nikon DS10 Color Digital Camera Head

6000 x 3984 (23.9 MP)(Nikon F-Mount) USB-C

i. 1 77013253 - Camera Cable USB 3.2 2x1 Locking C to C (2-Meter)

ii. 1 MQF52057 - AC Adapter 12v

iii. 1 DSUSB3PCIE - High Speed USB-3.2 Gen- 2x1 PCI Express Card

iv. 1 03158 - 6-Outlet Surge Suppressing Strip

v. 1 MQD43050 * - 1.8x Nikon F-Mount Adapter (DSF1.8) (Requires

MQD43000)

vi. 1 MQD43000 * - 1.0x Nikon F-Mount Adapter (DSF) (Sub-ISO)

d. NIS-Elements, Documentation [D] (Multi License)

i. Acquisition and Manual Analysis including Nikon Microscope drivers

e. Miscellaneous

i. 1 2800-4 - On Site Installation by our Factory Authorized Representative

ii. 1 2800-5 - Initial On Site Training Session

iii. 1 INST - Instruction Manuals

iv. 1 M2700KT - Lens Cleaning Kit

6. Quantity: One (1x) Nikon Ts2-Fl Inverted Microscope System w/ DS10 Camera.

7. Delivery Date: To be delivered after PO award at time agreed upon by Government and Vendor.

8. Period of Performance: N/A

9. Option(s): None