Attachment 001 Performance Work Statement and QASP.pdf
PDF 196 KB Posted
- Attached to
- Untargeted Metabolomics Service Federal contract opportunity
- Solicitation number
- 1232SA25Q0586
About this file
This Performance Work Statement (PWS) details a mass spectrometry-based untargeted metabolomics research project for the ERRC (likely the Eastern Regional Research Center). The government will ship 56 samples (45 for primary metabolism/complex lipids/biogenic amines, 5 QC samples, and 6 lipid samples) to a contractor for analysis. The contractor must process the samples using three analytical panels capable of providing data for over 1,000 validated compounds, with metabolite annotations requiring a confidence rating from levels 1-5. The total performance period is 180 days, with 30 days allocated for government preliminary tasks and 150 days for sample analysis and data reporting. Deliverables include raw data files, retention times, quantification masses, biochemical database identifiers, and adduct information, with metabolites reported as relative peak heights and potential absolute quantitation available upon request.
View the file
Other files for this federal contract opportunity
| File | Type | Posted |
|---|---|---|
| RFQ 1232SA25Q0586 Clauses and Provisions.pdf | ||
| Attachment 002 Wage Determination 2015_5791_R29.pdf | ||
| Attachment 003 Transmittal Summary.pdf |
On GovTribe
Work with this file on GovTribe
- Download the original file
- Contacts named in this file
- Similar government files
- Ask GovTribe AI about this file
Text version
Attachment 001 Performance Work Statement and QASP Mass Spectrometry-Based Untargeted Metabolomics
1. Background Scientists from the ERRC are evaluating the effect of different cooking oils on the metabolism of the gut microbiome as well as identifying microbial transformation of lipids.
2. Scope ERRC will use this acquisition to obtain mass spectrometry-based untargeted metabolomics results to further research goals.
3. Objectives Scientists from the DFFRU at the ERRC expect to receive untargeted metabolomics results and raw data files from this analysis. Results will be analyzed within the context of the experimental design and shared within manuscripts and presentations.
4. Tasks or Requirements:
4.1. Preliminary task by Government: ERRC scientists will ship filtered supernatant from bacterial cultures overnight on dry ice for mass spectrometry-based untargeted metabolomics and data analysis. Contractor is not responsible for shipping cost from Government to Contractor.
4.2. Preliminary task by Government: Breakdown of samples shipped by ERRC shall be as follows:
4.2.1. 45 Samples for primary metabolism, complex lipids, and biogenic amines
4.2.2. 5 QC samples
4.2.3. 6 lipids samples
4.3. Upon delivery samples will be thawed and processed for analysis.
4.4. Processed supernatants will be analyzed using three panels: primary metabolism, complex lipids, and biogenic amines. Combined these panels must be capable of providing data for over 1000 validated compounds and others of unknown chemical structure.
4.5. Data shall be reported with retention times, quantification masses, biochemical database identifiers, and adduct information. Metabolites shall be reported as relative peak heights, but absolute quantitation shall be available if requested
4.6. Metabolite annotations must be assigned a level of confidence rating (minimum 4, but ideally 5 levels), as follows:
4.6.1. Confirmed structure match based on MS, MS/MS, and RT of chemical standard.
4.6.2. Probable structure based on MS/MS match in a spectral database.
4.6.3. Tentative structure based on MS1 m/z database match.
4.6.4. Molecular formula identification based on isotope abundance, adduct formation etc.
4.6.5. Exact mass m/z.
4.7. Raw data files and methods shall be available to share with ERRC.
4.8. The total period of performance is 180 days.
4.8.1. 30 days after receipt of order will be allowed for the Government to complete Preliminary tasks.
4.8.2. The remainder of the tasks shall be completed within 150 days of sample delivery receipt.
5. Quality Assurance Surveillance Plan (QASP). The Government shall establish a QASP as a means of evaluating whether the Contractor is meeting the performance standards/quality levels identified in the
PWS to ensure that the government pays only for the level of services received
Attachment 001 Performance Work Statement and QASP Mass Spectrometry-Based Untargeted Metabolomics
Tasks Standards Acceptable Quality Level
(AQL)
Technical Requirements and
Tasks identified in Section 4
Standards as established in tasks identified in Section 4.
100%
File details come from the government source that posted it. Updated .