Att-Purchase_Description_75N95024Q00275.pdf

Version 11-30-2016-1 FAR Part 11—Describing Agency Needs

PURCHASE DESCRIPTION

Deep Proteomic Profiling of NIA Samples with the Proteograph Proteomics Platform

1. Statement of Need and Purpose:

The purpose of this requirement is to acquire an automated proteomic platform for quantitative and deep profiling of thousands of proteins in clinical and preclinical biofluids such as serum and plasma. This request is for the purchase of the Seer Proteograph Product Suite or equivalent and sample kits (Proteograph Assay Sample Kits and HT Sample Kits) which couples with mass spectrometry for unparalleled, unbiased, and deep protein biomarker discovery in clinical and pre-clinical biofluids.

2. Background Information and Objective:

The National Institute on Aging (NIA) is in need of an automated, comprehensive, unbiased, and quantitative proteomics platform for the discovery proteomic biomarkers in biofluids acquired from multiple research laboratories. The SEER Proteograph Suite is designed to overcome the immense dynamic range problem of biofluids such as serum and plasma, which spans over 10 orders of magnitude and masks the ability of mass spectrometry-based technologies to detect low-abundance proteins, including the vast majority of proteins leaked from tissues, which are the most promising source of biomarkers of blood biomarkers. Secondly, the Seer Proteograph Analysis Software (PAS), included in the suite, tackles the issue of the scalability of large-scale proteomic studies, which are limited by the immense computational power required for analysis of large omic datasets. The PAS software enables determination of significant biomarkers, pathway analysis, visualizations, and other features for hundreds of proteomic datafiles within a matter of hours within the cloud.

3. Generic Name of Product:

Automated Nanoparticle-Based Proteomic Sample Preparation Robot and Sample Kits

4. Purchase Description:

Seer Proteograph Product Suite, including the SP100 Automation Instrument with XT Upgrade (S55R4008), Proteograph™ XT Installation Bundle (S55R4009), Proteograph Analysis Suite Base Package (S55R1204), SP100 12 Month Warranty (S55R1304), Proteograph™ XT Assay Kits 160 Samples (S55R4002), Proteograph™ XT PQR Kit (S55R4007), and freight (FRT- 1000010).

5. Salient characteristics:

• Two-nanoparticle panel

• Automation instrument

• Software to perform unbiased, deep proteomic analysis at scale in a matter of hours.

• Ability to provide the combination of unbiased, deep, rapid, and large-scale access to the proteome.

Version 11-30-2016-1 FAR Part 11—Describing Agency Needs

• Ability to provide insight into protein variation at the peptide level and protein-to-protein interactions at depth and scale for unbiased and deep proteomics which is otherwise unattainable with existing approaches.

• Designed to enable broad adoption across a wide variety of customers in both decentralized and centralized settings.

• Identify and distinguish protein variants at the peptide level.

• Identify and quantify post-translational modifications and protein-to-protein interactions.

• Be compatible across a wide range of laboratory workflows, sample processing and detection methods.

6. Quantity:

One protegraph platform and associated items are requested, as well as 10 sample kits (160 samples each).

7. Delivery Date:

Seer, Inc. shall supply all deliverables. The instrument shall be installed and onsite training shall be completed within 15 weeks after receipt of order.

8. Government Responsibilities:

Dr. Basisty will examine and inspect all upon completion of installation. Contractor will require access to BRC, installation site (to be determined), to install the instrument and provide on-site training for laboratory staff. Contractor’s installers will be required to coordinate with Dr. Basisty in advance of arrival so that access may be granted. Contractor will not require access during federal holidays or weekends.

9. Other Considerations:

Contractor shall install and provide onsite training by Seer Inc. installers and training instrucators.