A04_SPECIFICATIONS_GC-MSD_06-23-2026 1232SA26Q0925.docx

SPECIFICATIONS

1.0 Specifications Description:

The USDA ARS Biosciences Research Laboratory, Food Animal Metabolism Research Unit and the Sugarbeet Research Unit, Fargo, North Dakota have need for a bench-top single quadrupole mass spectrometer configured with a gas chromatograph with autosampler for liquid injection, pyrolyzer unit with components, and solid phase micro-extraction capabilities. A satisfactory bid must meet the following requirements:

Single Quadrupole Mass Spectrometer with following; Quantity 1, Unit Each:

1. Inert electron ionization (EI) source

2. Electron multiplier detector

3. Ion gauge

4. Foreline vacuum pump - dry scroll pump or equivalent

5. Turbo High Vacuum Pump

6. Maximum column ID of 0.32 mm or greater

7. Setup for Helium Carrier gas

8. Acquisition: Full ion scan and selected ion monitoring (SIM) capability

9. Mass Range: At least 1.2 – 1000 atomic mass units or higher high mass limit

10. Scan speed of 20,000 u/second (u=unified atomic mass units)

11. All accessories and kits needed for install and operation

12. Uninterruptable Power supply, 6kV; Quantity 1, Unit Each Gas Chromatograph; Quantity 1, Unit Each:

1. Capillary flow technology

2. GC Diagnostics functionalities

3. 2 injector ports, specified below

4. Maximum temperature ramps of at least 90°C/min

5. Operating temperature range

a. At least 3-4°C above ambient temperature to 450°C

6. Oven Cool down

a. 450°C to 50°C in 4.0 minutes (22°C ambient)

7. Peak Area Repeatability <0.5% RSD

8. Retention Time Repeatability <0.01%

9. Typical Pressure Control <0.01 psi

10. Oven exhaust deflector

11. All accessories and kits needed for install and operation Autosampler and Injector Ports; Quantity 1 with components, Unit Each:

1. Standard liquid and headspace (gas) autosampler injector and vial trays (2mL and 10mL) with smart rail system for multipurpose use components below; quantity 1, unit each

a. Universal sample tray holder with trays for 2 mL vials and 10 mL vials

b. Universal syringe module for liquid injection 10 microliter or less

c. Standard syringe wash station

d. Sample vial tray Pyrolysis injection (see pyrolyzer specifications)

2. Split/Splitless injector modules; quantity 2, unit each

a. Install kits for 2 injector ports; quantity 2, unit each

3. Adaptor kit for gas injection for pyrolyzer on split/splitless injector; quantity 1, unit each Solid phase micro extractor system (SPME) ; Quantity 1 with components below, Unit Each:

1. SPME fiber conditioning station

2. SPME fiber kit with fibers and test mixture for autosampler and GC

3. SPME heater/agitator for 10 mL vials

a. Adapters for heater/agitator Pyrolyzer Unit and Components; Quantity 1 with components, Unit Each:

1. Multi Shot Pyrolyzer with install kit; quantity 1, unit each:

a. Must consist of a ceramic micro furnace design with temperature reproducibility of +/- 0.1ᵒ C or equivalent.

i. Thermocouple temperature measurement is designed into the micro furnace or equivalent for temperature calibration.

b. Unit mounts onto the GC inlet system with no external transfer lines.

c. Settable and programmable from ambient room temperature + 10ᵒ C up to 1050ᵒ C in 1ᵒ C increments with +/- 0.1ᵒ C stability.

d. GC Interface must be settable from 40ᵒ C to 450ᵒ C in 1ᵒ C increments with +/- 0.1ᵒ C stability.

e. Must meet the following guaranteed performance requirements:

i. Using flash pyrolysis of 25 µg of a polystyrene solution containing methyl stearate (5 µg/µl) at 550ᵒC, the relative standard deviation (RSD%) of the peak area ratios of the styrene trimer and methyl stearate must be ≤ 2%.

f. The cooling time of the furnace or equivalent from 800ᵒC to 50ᵒC must be ≤ 10 minutes to maximize sample throughput.

g. Must include a 2-year warranty.

2. Needs for Auto Sampler

a. A single sample tray must be capable of holding 48 samples; quantity 1, unit each

b. Sample cups must be made from stainless steel that has been deactivated.

c. Sample cup volume must be ≤ 80µl.

d. Must allow automated unattended analysis of 48 samples, including single-shot analysis and double-shot analysis.

3. Cryogenic Trapping (Cryo-Trap) ; quantity 1, unit each:

a. A Cryo-Trap device utilizing liquid nitrogen (LN2).

i. The Cryo-Trap must be able to reach temperatures as low as -196ᵒC so that CO2 may be effectively cryo-trapped.

ii. The Cryo-Trap must spray LN2 directly onto the analytical column in a very narrow band of < 5mm.

iii. The Cryo-Trap must utilize gaseous nitrogen flow through a thermal exchange coil (placed in LN2) with the ability to vary the flow rate from 3-7 L/min, allowing choice of desired cooling temperatures.

1. A optional thermal exchange coil must be available for use with a 30L LN2 Dewar (user-supplied) to allow unattended operation with the Auto Sampler.

b. Cryo-Trap must be software-controlled using the same software as the pyrolyzer.

4. Multi-Functional Splitless sampler (see above); quantity 1, unit each:

a. Used with the Multi-Shot Pyrolyzer, which allows for the F-Splitless injection method, backflush, solvent cut, and improves peak separation.

b. This device must allow the user to perform splitless analysis for Flash pyrolysis (Single-shot analysis) and Double-shot analysis

5. Software; quantity 1, unit each (see below in workstation section):

a. Full license

b. Must be capable of controlling all set points of the pyrolyzer and the autosampler or work seamlessly with GC-MSD software.

c. Must be capable of controlling the multiple modes of operation, including:

i. Evolved Gas Analysis (EGA)

a. EGA is defined as analyzing a sample using a direct connection from the S/SL GC injection port and the MS using a 2.5 meter, 0.15mm i.d. deactivated stainless steel capillary tube.

ii. Pyrolysis (PY)

iii. Reactive Pyrolysis (RxPY)

iv. Heart cuts based on the EGA Thermograms

v. High pressure, high temperature reaction chemistry Search Library; quantity 1, unit each:

a. Software for microplastic library searching must be available with the following specifications:

i. Incorporate search algorithms that allow spectral library searching of pyrograms based on C1-C10, C1-C20, and C1-C40 carbon groupings,

ii. Automatically create a calibration curve for the registered polymers (ISTD or ESTD) based on the analytical results from the reference polymer mixture

iii. Automatically performs quantification with the results instantly reported for the environmental microplastics.

6. Microplastics calibration kit (MPCS) with two different diluents (SiO2 or CaCO3); quantity 1, unit each:

a. Both MPCS contain a few µg each of commonly used 12 polymers, which are homogeneously dispersed with a solid diluent for easy weighing on semi-microbalances.

Workstation for Diagnostics, Instrument Control, Data Acquisition, Data Processing

1. Workstation system

a. Computer with monitor; quantity 1, unit each

b. Software license for equipment control; quantity 1, unit each

i. Instrument control for GC, MSD, Autosampler, SPME, Pyrolyzer

ii. System parameter checks including vacuum levels

iii. System Diagnostics

iv. System calibration or tuning equivalent

v. Acquisition and processing included for all components

1. Instrument and acquisition method building for collection of full scan and selected ion monitoring

c. Second software license for remote or offline data analysis and processing; quantity 1, unit each

d. NIST MS Library Bundle or equivalent; quantity 1, unit each

System Installation and Performance

1. Onsite full equipment setup with all components and install kits; quantity 1, unit each

2. Specification Testing/Compliance for all components; quantity 1, unit each

3. Onsite product familiarization training for all components of 2-3 days; quantity 1, unit each

4. Performance

a. Acquisition in full scan and selected ion monitoring

b. 100 fg, 1 ul OFN; injected: IDL 10 fg

c. Meets all compliance and specification testing during installation

a. Performance specification kit; quantity 1, unit each

5. Manufacturer’s extended warranty or service equivalent for gas chromatograph and single quadrupole mass spectrometer for 1 year; quantity 3, unit year

a. Onsite corrective maintenance visits including required factory certified replacement parts, labor, and travel with a target of three business days onsite response time

b. Annual standard preventive maintenance visit and a standard preventive maintenance kit

c. Priority status for technical support telephone inquiries Options:

1. 3, 1-year Extended Warranty or service equivalent for Autosampler Injector and Sample System

a. Onsite corrective maintenance visits including required factory certified replacement parts, labor, and travel with a target of three business days onsite response time

b. Annual standard preventive maintenance visit and a standard preventive maintenance kit

c. Priority status for technical support telephone inquiries

2. 3, 1-year Extended Warranty or service equivalent for solid phase microextractor (SPME) and components

a. Onsite corrective maintenance visits including required factory certified replacement parts, labor, and travel with a target of three business days onsite response time

b. Annual standard preventive maintenance visit and a standard preventive maintenance kit

c. Priority status for technical support telephone inquiries

3. 3, 1-year Extended Warranty or service equivalent for pyrolyzer and components

a. Onsite corrective maintenance visits including required factory certified replacement parts, labor, and travel with a target of three business days onsite response time

b. Annual standard preventive maintenance visit and a standard preventive maintenance kit

c. Priority status for technical support telephone inquiries

4. Spare electron ionization source

2.0 Place of Delivery

USDA-ARS Edward T. Schafer Agricultural Research Center 1616 Albrecht Blvd N Fargo, ND 58102 There is a receiving area and forklift available for moving items at delivery but there is not a loading dock. Items will remain in receiving area until contractor is onsite to check shipment and will move to installation site at time of install, pallet jacks will be available for this purpose.

3.0 Delivery Schedule

1616 Albrecht Blvd N Fargo, ND 58102

2-3 months

4.0 Additional Requirements

0. Are there any Security requirements? Is an escort needed for delivery? N/A

0. Is Installation required? Yes, in cost estimate and specified in specifications above

0. Is Assembly required? Yes, but part of installation

0. Is a warranty required? Yes, in cost estimate and specified in specifications including purchasing a 3-year extended warranty/service with other options

0. Is training required for use of this item? Yes, but part of installation cost and specified above. Typically training onsite after installation of 2-3 days.

0. Is maintenance required on this item? Yes, but only as part of warranty and included in cost estimate and specifications above.