A04_REQ 1174008 - SPECS - LEO SYSTEM AUTO WESTERN BLOTTING 1232SA26Q1092.docx

SPECIFICATION OF SUPPLIES

1.0 Specifications Description

USDA – ARS – RAFSRU conducts research on molecular analysis of plant and animal tissues and quantification of biomolecules such a proteins in cell lysates via immunoblotting. USDA – ARS – RAFSRU requires an automated immunoassay platform capable of high‑throughput protein detection and characterization. The instrument should be able to conduct immunoblotting for up to 96 samples at one time. All steps of sample loading, antigen immobilization, immunodetection and imaging should be automated. The system should be capable of normalization for total protein in the lane. This equipment is essential to increase rigor, reproducibility, and efficiency and simplify labor‑intensive steps involved in traditional Western blot assays. It is expected to minimize user‑dependent variability and improve overall data reliability. The instrument should meet or exceed the specification of the Leo Simple Western system (Biotechne).

High-throughput quantitative Western analysis, computer and software

Quantity: 1

Unit Type: Each

Minimum Requirements:

A. System Requirements:

1. An instrument that performs size-based separation and immune detection of proteins in a fully automated manner.

2. Instrument should be able to process around 90-100 samples per run in a fully automated manner. Should conduct electrophoresis within capillaries.

3. Should be able identify and quantify target proteins at a highly specific level. System should provide customizable primary antibody options based on user needs.

4. System should have 3-4 log dynamic range of detection.

5. Ability to employ chemiluminescence and fluorescence to detect target proteins with low background readings.

6. Inter instrument and intra-run CV should be less than 10% CV.

7. Should be able to detect multiple analytes and should require low sample input (< 5ul sample volume).

8. Should allow re-probing of second analyte after removal of the first primary antibody following detection steps.

9. Should perform an assay in less than 5 hours.

10. Should be able to resolve various size ranges and have established separation modules (12-230 kDa, 2-40 Kda, etc.).

11. Provide a workstation with seamless software assay design, instrument programming and quantitative analysis of results.

12. Dimensions should be accommodatable in existing lab space.

13. Provide comprehensive installation and training, customer support for troubleshooting run setup and data output issues and provide maintenance visits and coverage of repair parts.

B. Incidental Services:

1. Install one automated Western instrument, computer and software at location per Section 2.0.

2. Training Requirements: Training shall occur immediately following installation.

3. One year of additional phone and virtual meeting support shall be available.

2.0 Place of Delivery

Delivery FOB: Responsive Agricultural Food Systems Research Unit, 498 Olsen Blvd, TAMU 2250, College Station, TX 77843

A loading dock is available and located at the back of the building.

Training will occur on-site.

3.0 Delivery Schedule and Incidental Service Schedule

4.0 Additional Requirements

Warranty: System shall have a minimum of a 1-year warrant from completion of installation and a complete service manual Warranty Requirements for all components.

Facility Access: No badges or background checks are required. A federal employee will be assigned to authorize the contractor and their workers entry. Contractor employees will be escorted and monitored throughout the building. Work shall be performed Monday through Friday between the hours of 8:30 am to 4:00 pm.