B. MR - Sources Sought Notice - 75N95024Q00474.pdf

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REGULAR SOURCES SOUGHT NOTICE

1. Solicitation Number: 75N95024Q00474

2. Title: Real Time semi-quantitative PCR (RT-qPCR) & digital PCR (dPCR)

System or equal

3. Classification Code: 6640 Laboratory Equipment and Supplies

4. NAICS Code: 334516 Analytical Laboratory Instrument Manufacturing

5. Size Standard: 1,000 employees

6. Description:

This is a Sources Sought notice. This is NOT a solicitation for proposals, proposal abstracts, or quotations. The purpose of this notice is to obtain information regarding the availability and capability of all qualified sources to perform a potential requirement.

This notice is issued to help determine the availability of qualified companies technically capable of meeting the Government requirement and to determine the method of acquisition, and availability of domestic sources manufactured in the United States in sufficient and reasonably available commercial quantities and of a satisfactory quality. It is not to be construed as a commitment by the Government to issue a solicitation or ultimately award a contract. Responses will not be considered as proposals or quotes. No award will be made as a result of this notice. The Government will NOT be responsible for any costs incurred by the respondents to this notice. This notice is strictly for research and information purposes only.

For equipment/supply requirements, responses must include: (1) the place of manufacturer (i.e. address if supply/equipment is a domestic end product and include country of manufacture), as well as (2) the size status of the manufacturer under the applicable NAICS code (i.e. address Non-Manufacturer Rule).

Background: The Infections of the Nervous System Section, INSS lab has developed sensitive novel assays to measure genomic viral RNAs and their transcriptional activity within biological fluids from individuals with neurodegenerative disease as well as people living with HIV. Our laboratory also frequently performs nucleic acid extractions from experimental samples, followed by quantitation of the expression of a large panel of genes related to neuroinflammation and neurodegeneration. The RT-qPCR and dPCR systems will be used for a variety of assays to map the relevant gene expression profiles in healthy and disease states to identify avenues for therapeutic intervention.

Purpose and Objectives: The purpose of this requisition is to purchase a Real Time semi-quantitative PCR (RT-qPCR) and digital PCR (dPCR) system bundle capable of multiplexed amplification and detection of gene expression. These systems will be used to document nucleic acid levels in experimental and clinical samples for the Infections of the Nervous System Section, INSS, National Institute of Neurological Disorders and Stroke, NINDS.

Specific Requirement: qPCR bundle: Real Time qPCR and digital PCR sytem

Contractor should provide:

9003204 QIAcuity One 5plex PLT-FUL-5 9003197 QIAquant 96 5plex (115V) SYS-DFUL-5 9003078 QIAcube Connect MDx SYS-FUL-5

250011 QIAcuity Nanoplate 8.5k 24-well (10) 250021 QIAcuity Nanoplate 8.5k 96-well (10) 250031 QIAcuity Nanoplate 26k 8-well (10) 250001 QIAcuity Nanoplate 26k 24-well (10) 250102 QIAcuity Probe PCR it (5mL) 250098 QIAcuity Nanoplate Tray (2) 250299 QIAcuity Demo Assay 209003 qPCR adhesive plate foil (100) 209002 qPCR 96-well plate, white, skirted (10) 9003094 QIAsphere Connectivity Package B 9026181 O-Ring Change Tool for QIAcube Connect 900395 Starter Pack, QIAcube 900394 Rotor Adapters (10 x 24) 990382 Sample Tubes CB (2ml) 990381 Sample Tubes RB (2ml)

5 year preventive maintenance and 5 year warranty on all parts and labor Included in the quote. Installation and training

The dPCR product must include:

• Multiplex detection of 5 different gene targets

• Partition-based amplification of RNA and DNA with single copy sensitivity

• Low inter- and intra-plate variability, with coefficient of variation

(%CV) below 10%

• Sensitive detection of genetic mutations, copy number variation

(CNV), gene expression

• All-in-one automated platform that does not require user to transfer samples from compartment to compartment or machine to machine after run is started; no accessory equipment required

• Total run time <4 hours from cDNA generation to analysis

• Software Analysis Suite that is accessible via local area network

(LAN) to multiple computers

• Total bench footprint not to exceed 16” X 28” X 20” (LXWXH)

• 5-year service contract for repair, maintenance, parts and labor

Real-Time qPCR product must include:

• Multiplex detection of 5 different gene targets

• Low inter- and intra-plate variability, with coefficient of variation

(%CV) below 10%

• All-in-one automated platform that does not require user to transfer samples from compartment to compartment or machine to machine after run is started; no accessory equipment required

• Analysis software capable of quality control (melting temperature analysis, inter-replicate variability, Ct threshold determination) and quantifying fold change in gene expression

• Ability to switch between 96-well and 384-well blocks between runs

• 5-year service contract for repair, maintenance, parts and labor

Anticipated period of performance or Delivery: NINDS has a need for delivery within 30-60 days after receipt of order (ARO).

Other important considerations: In case domestic sources are available and capable of fulfilling the Government’s need, and a future solicitation is published, the Government will use evaluation preferences in accordance with FAR 25.

Capability statement /information sought.

Small business concerns that believe they possess the capabilities to provide the required products or services should submit documentation of their ability to meet each of the project requirements to the Contracting Officer. The capability statement must specifically address each of the project requirements separately. Additionally, the capability statement should include 1) the total number of employees, 2) the professional qualifications of personnel as it relates to the requirements outlined, 3) any contractor GSA Schedule contracts and/or other government-wide acquisition contracts (GWACs) by which all of the requirements may be met, if applicable, and 4) any other information considered relevant to this program. Small businesses must also provide their Company Name, UEI number, Physical Address, and Point of Contact Information.

Interested small businesses are required to identify their type of business, applicable North American Industry Classification System (NAICS) Code, and size standards in accordance with the Small Business Administration. The government requests that no proprietary or confidential business data be submitted in a response to this notice.

However, responses that indicate the information therein is proprietary will be properly safeguarded for Government use only. Capability statements must include the name and telephone number of a point of contact having authority and knowledge to discuss responses with Government representatives. Capability statements in response to this market survey that do not provide sufficient information for evaluation will be considered non-responsive. When submitting this information, please reference the solicitation notice number.

The respondent must also provide their UEI number, organization name, address, point of contact, and size and type of business (e.g., 8(a), HubZone, etc., pursuant to the applicable NAICS code and any other information that may be helpful in developing or finalizing the acquisition requirements.

One (1) copy of the response is required and must be in Microsoft Word or Adobe PDF format using 11-point or 12-point font, 8-1/2” x 11” paper size, with 1” top, bottom, left and right margins, and with single or double spacing.

The information submitted must be in and outline format that addresses each of the elements of the project requirement and in the capability statement /information sought paragraphs stated herein. A cover page and an executive summary may be included but is not required.

The response is limited to ten (10) page limit. The 10-page limit does not include the cover page, executive summary, or references, if requested.

The response must include the respondents’ technical and administrative points of contact, including names, titles, addresses, telephone and fax numbers, and e-mail addresses.

All responses to this notice must be submitted electronically to the Contract Specialist and Contracting Officer. Facsimile responses are NOT accepted.

The response must be submitted via email to the Contract Specialist, Frederick Weddington (frederick.weddington@nih.gov).

The response must be received on or before 7/30/2024, 9:00 A.M., Eastern Time.

“Disclaimer and Important Notes: This notice does not obligate the Government to award a contract or otherwise pay for the information provided in response. The Government reserves the right to use information provided by respondents for any purpose deemed necessary and legally appropriate. Any organization responding to this notice should ensure that its response is complete and sufficiently detailed to allow the Government to determine the organization’s qualifications to perform the work.

Respondents are advised that the Government is under no obligation to acknowledge receipt of the information received or provide feedback to respondents with respect to any information submitted. After a review of the responses received, a presolicitation synopsis and solicitation may be published in Federal Business Opportunities. However, responses to this notice will not be considered adequate responses to a solicitation.

Confidentiality: No proprietary, classified, confidential, or sensitive information should be included in your response. The Government reserves the right to use any non-proprietary technical information in any resultant solicitation(s).”

mailto:frederick.weddington@nih.gov