Amnd 0001_signed.pdf

(x)

75N95024Q00467 x x copies of the amendment; (b) By acknowledging receipt of this amendment on each copy of the offer submitted ; or (c) By separate letter or electronic communication which includes a reference to the solicitation and amendment numbers. FAILURE OF YOUR ACKNOWLEDGEMENT TO BE

RECEIVED AT THE PLACE DESIGNATED FOR THE RECEIPT OF OFFERS PRIOR TO THE HOUR AND DATE SPECIFIED MAY RESULT IN REJECTION OF YOUR

OFFER. If by virtue of this amendment you desire to change an offer already submitted , such change may be made by letter or electronic communication, provided each letter or electronic communication makes reference to the solicitation and this amendment, and is received prior to the opening hour and date specified.

x

NINDS

Bethesda, MD 20892-7511 Disorders and Stroke National Institute of Neurological National Institutes of Health

08/29/20240001

13. THIS ITEM ONLY APPLIES TO MODIFICATION OF CONTRACTS/ORDERS. IT MODIFIES THE CONTRACT/ORDER NO. AS DESCRIBED IN ITEM 14.

12. ACCOUNTING AND APPROPRIATION DATA (If required) is not extended.is extended, Items 8 and 15, and returning

Offers must acknowledge receipt of this amendment prior to the hour and date specified in the solicitation or as amended , by one of the following methods: (a) By completing

The above numbered solicitation is amended as set forth in Item 14. The hour and date specified for receipt of Offers

11. THIS ITEM ONLY APPLIES TO AMENDMENTS OF SOLICITATIONS

FACILITY CODE CODE

10B. DATED (SEE ITEM 13)

10A. MODIFICATION OF CONTRACT/ORDER NO.

9B. DATED (SEE ITEM 11)

9A. AMENDMENT OF SOLICITATION NO.

CODE

8. NAME AND ADDRESS OF CONTRACTOR (No., street, county, State and ZIP Code)

7. ADMINISTERED BY (If other than Item 6)CODE 6. ISSUED BY

PAGE OF PAGES

4. REQUISITION/PURCHASE REQ. NO.3. EFFECTIVE DATE2. AMENDMENT/MODIFICATION NO. 5. PROJECT NO. (If applicable)

1. CONTRACT ID CODE

AMENDMENT OF SOLICITATION/MODIFICATION OF CONTRACT

08/29/2024

CHECK ONE A. THIS CHANGE ORDER IS ISSUED PURSUANT TO: (Specify authority) THE CHANGES SET FORTH IN ITEM 14 ARE MADE IN THE CONTRACT

B. THE ABOVE NUMBERED CONTRACT/ORDER IS MODIFIED TO REFLECT THE ADMINISTRATIVE CHANGES (such as changes in paying office, C. THIS SUPPLEMENTAL AGREEMENT IS ENTERED INTO PURSUANT TO AUTHORITY OF:

D. OTHER (Specify type of modification and authority) appropriation data, etc.) SET FORTH IN ITEM 14, PURSUANT TO THE AUTHORITY OF FAR 43.103(b).

E. IMPORTANT: Contractor is not is required to sign this document and return __________________ copies to the issuing office.

ORDER NO. IN ITEM 10A.

14. DESCRIPTION OF AMENDMENT/MODIFICATION (Organized by UCF section headings, including solicitation/contract subject matter where feasible.)

The purpose of this Amendment is to provide responses to vendor-submitted questions.

All other terms and conditions remain unchanged.

Attachment 1: Questions and Answers

Attachment 2: Revised Purchase Description

16A. NAME AND TITLE OF CONTRACTING OFFICER (Type or print)15A. NAME AND TITLE OF SIGNER (Type or print)

15C. DATE SIGNED 16B. UNITED STATES OF AMERICA 15B. CONTRACTOR/OFFEROR 16C. DATE SIGNED

(Signature of person authorized to sign) (Signature of Contracting Officer)

EVAN C. FEELY

STANDARD FORM 30 (REV. 11/2016)

Prescribed by GSA FAR (48 CFR) 53.243

Previous edition unusable

Except as provided herein, all terms and conditions of the document referenced in Item 9 A or 10A, as heretofore changed, remains unchanged and in full force and effect .

Questions and Answers

Request for Quotations (RFQ) Number: 75N95024Q00467

Project Title: Molecular Devices brand-name amplifiers and digitizers for use in electrophysiology experiments

Question Number Question and Answer

1.

Question: There are two versions of pCLAMP 11 software: Regular and Advanced. Your Purchase Description does not say which one you want.

Answer: NINDS requires the advanced version of the pCLAMP 11 software.

2.

Question: In section 4 of the Purchase Description it says that you need 1 Multiclamp 700B and then in section 5 it says you need 2 Multiclamp 700B’s. Please advise how many as I want to be sure to quote the correct quantities.

Answer:

NINDS only requires one (1) Multiclamp 700B amplifier.

3.

Question: While we provide inside delivery, we do NOT do installation. Please clarify whether this is necessary.

Answer: Installation is not required.

Version 11-30-2016-1 FAR Part 11—Describing Agency Needs

PURCHASE DESCRIPTION

Molecular Devices brand-name amplifiers and digitizers for use in electrophysiology experiments

1. Statement of Need and Purpose:

The purpose of this requirement is to acquire Molecular Devices brand-name electrophysiological equipment (amplifiers and digitizers), which will be used in examining the cellular excitability of neurons located in the midbrain dopamine system and basal ganglia.

2. Background Information and Objective:

Research in the National Institute of Neurological Disorders and Stroke (NINDS) Cellular Neurophysiology Section (CNS) focuses on circuit and cellular properties of neurons located in the midbrain dopamine system and basal ganglia. As a major part of this research, the CNS is interested in the physiology of dopamine neurons located in the substantia nigra pars compacta (SNc) that undergo selective cell death in Parkinson’s patients. Specifically, it is interested in understanding how synaptic and intrinsic conductances expressed on these neurons (particulary calcium permeable conductances, i.e., voltage gated calcium channels and glutamate activated receptors) differ among subpopulations dopamine neurons. The hope is that this information may provide insight to why SNc dopamine neurons are particularly vulnerable in Parkinson’s disease. CNS’ goal is to identify novel physiological and genetic markers in single SNc dopamine neurons that may be useful in defining subpopulations of these neurons in a meaningful way.

In order to study the action potential firing properties and physiology of dopamine system neurons, the CNS laboratory uses electrophysiological approaches. This approach allows it to have direct access to the activity of neurons and to have very precise control over the experimental parameters. In order to record the electrical activity of these neurons, the CNS will need to use high-precision amplifiers that allow it to accurately measure submillivolt changes in the membrane potential of cells. This activity will be recorded on computers using digitizers.

3. Generic Name of Product

Amplifiers and digitizers for use in electrophysiology experiments

4. Brand Name Product Information:

Molecular Devices Multiclamp 700B, Quantity 1 Molecular Devices Digidata 1550B4, Quantity 2 PClamp software (advanced module), Quantity 2

5. Salient characteristics

Amplifiers

The NINDS Cellular Neurophysiology Section requires one (1) amplifier that meets the following requirements:

• The system must have external trigger command or userprogrammable voltage threshold for rapid, automatic mode-switching between current and voltage-clamp and a means of detecting current or voltage oscillations and automatically disabling or intelligently reducing compensation settings to protect the cell from damage.

• The system must be able to automatically injects a compensatory current over a user-defined time course.

• The system must also include a dual headstage system to record simultaneously in two independent channels that integrate both current-to-voltage and voltage-following circuitry.

• The system must also have computer control software that providing automation of pipette offset, fast/slow electrode capacitance compensation, whole-cell capacitance compensation, series resistance correction, pipette capacitance neutralization and bridge balance.

Digitizers

The NINDS Cellular Neurophysiology Section also requires two (2) digitizers that meet the following requirements:

• The system must be compatible with pClamp software that is used for acquisition of electrophysiology.

• The system must be able to sample at 500 kHz per channel.

• The system must have a humsilencer that eliminates 50/60 Hz line-frequency noise pattern associated with high-frequency line harmonics.

• The system must be low-noise data acquisition system plus 4 channels humsilencer adaptive noise cancellation

6. Warranty:

The Contractor shall warrant that that the Equipment will be free from defects for a period of twelve (12) months from the date of installation, inspection by the Government, and acceptance.

7. Delivery Requirements:

The Contractor shall deliver all required equipment within six (6) months after receipt of order.

Delivery must be FOB Destination and include inside delivery. The delivery location is Building 35 on the National Institutes of Health (NIH) main campus.

Coordination of delivery will be performed with the Contracting Officer’s Representative (COR) at the time the equipment is ready to be shipped. The equipment shall be delivered between the hours of 8:00am and 5:00pm, Bethesda, MD local prevailing time, Monday through Friday. The full delivery address is provided below:

National Institutes of Health Campus National Institutes of Neurological Disorders and Stroke Cellular Neurophysiology Section Building 35 35 Convent Dr, Rm 3C-1002 Bethesda, MD 20814