Purchase Description - 75N95024Q00359.pdf

Version 11-30-2016-1 FAR Part 11—Describing Agency Needs

PURCHASE DESCRIPTION

1. Statement of Need and Purpose: CosMx and GeoMx consumables

2. Background Information and Objective: CosMx technology by Nanostring offers a valuable tool in vascular aging studies by enabling highly sensitive and multiplexed measurement of key molecular pathways involved in vascular health and aging. Through the simultaneous quantification of multiple genes associated with vascular function, inflammation, oxidative stress, and endothelial dysfunction, CosMx technology provides researchers with a comprehensive understanding of the complex molecular mechanisms underlying vascular aging processes. This high-throughput and customizable approach allow for the identification of novel biomarkers, elucidation of disease mechanisms, and development of targeted interventions aimed at preventing or reversing vascular aging-associated pathologies, ultimately facilitating the advancement of precision medicine in the field of cardiovascular health.

GeoMx technology by NanoString offers a powerful platform for studying aging by enabling spatially resolved transcriptomic analysis of tissue samples. This technology allows researchers to investigate the intricate molecular changes that occur within specific regions of tissues involved in the aging process, such as the vasculature, nervous system, and immune system. By precisely mapping gene expression patterns at the cellular level, GeoMx technology provides insights into the spatial organization of aging-related pathways, cellular interactions, and tissue microenvironments. This spatially resolved approach facilitates the identification of novel biomarkers, elucidation of tissue-specific aging signatures, and discovery of potential therapeutic targets for age-related diseases, thus advancing our understanding of the molecular mechanisms underlying aging and age-related pathologies.

Spatial transcriptomics is crucial in vascular senescence research to pinpoint senescent cells within the intricate architecture of blood vessels. As aging progresses, vascular tissues undergo significant changes, including cellular senescence, which contributes to various age-related diseases. Traditional methods of identifying senescent cells often lack spatial resolution, making it challenging to precisely locate these cells within tissues. Spatial transcriptomics techniques, however, offer the ability to map gene expression patterns within the context of tissue structure, allowing researchers to identify and characterize senescent cells in their native microenvironment. This spatially resolved approach provides valuable insights into the distribution and interactions of senescent cells within vascular tissues, facilitating a deeper understanding of their role in vascular aging and age-related pathologies. For these reasons, the consumables provided by Nanostring are required for our research.

3. Generic Name of Product: Spatial transcriptomics.

4. Purchase Description:

Item # Description Quatnities 100473 GeoMx DSP Collection Plate 4pk EA 1.00 100474 GeoMx Instrument Buff Kit PCLN EA 2.00 121401103 GeoMx NGS RNA WTA Mm EA 2.00 121300303 GeoMx Nuclear Stain Morp Kit EA 1.00 121300313 GeoMx RNA Slide Prep FFPE PCLN EA 1.00

Version 11-30-2016-1 FAR Part 11—Describing Agency Needs

121400205 GeoMx Seq Code Pack_ABCD EA 1.00 SH0003 S&H 1 - 8 Kits / Slides EA 1.00

100480 CMx RNA Inst Buffer Kit, Small EA 4.00 121500003 CMx Mm Neuro Panel RNA Kit EA 1.00 121500004 CMx RNAse Inhib, 2 sl Kit EA 4.00 121500006 CMx Slide Prep Kit, FFPE RNA EA 2.00 121500024 CMx Mm Neuro Cell Seg Kit, RNA EA 1.00 121500040 CMx Mm Univ Cell Panel RNA Kit EA 1.00 121500042 CMx Mm Univ Cell Seg Kit, RNA EA 1.00 121500044 CMx Mm CD68 Marker Kit, RNA EA 1.00 122000061 CosMx SMI Flow Cell, 4 sld EA 2.00 122000152 CMx Cleaning Tray Kit EA 4.00 122000156 CMx RNA 1000plx 2sl Img Tray EA 4.00 SH0004 S&H 9 - 32 Kits / Slides EA 1.00

5. Salient characteristics: The exclusive availability of CosMx and GeoMx consumables through

NanoString stems from several factors, including the intricate technology and specialized expertise required for their development, manufacturing, and support. NanoString's direct distribution ensures strict quality control and comprehensive technical assistance, ensuring optimal performance and user experience with their platforms. Moreover, NanoString's proprietary technologies and intellectual property rights associated with CosMx and GeoMx consumables may necessitate restrictions on third-party distribution. This direct sales approach enables NanoString to establish closer relationships with researchers, facilitating feedback-driven product improvements and fostering a loyal customer base while maintaining control over the distribution and support of their innovative technologies.

6. Quantity: Displayed above.

7. Delivery Date: 30 days after receipt of order.