D-Attachment-Questions and Answers 75N95022Q00098.pdf
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- Solicitation number
- 75N95022Q00098
About this file
This combined synopsis/solicitation requests quotations for a firm fixed-price purchase order to generate up to 200 induced pluripotent stem cell lines from patient blood samples. The National Institute on Drug Abuse requires the lines be generated over a two-year period using fully automated reprogramming and expansion methods. Quotations are due by April 14th, 2022 and the period of performance is one base year starting July 1st, 2022 with an option to extend for one additional year. Technical capability, past performance, and price will be evaluated with technical and past performance considered more important than price. The Dun & Bradstreet Number, Taxpayer Identification Number, and small business certification must be included.
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Text version
Question Response
"Contractor shall generate a collection of up to 200 engineered iPSC lines from fresh whole blood or frozen PBMCs using an automated system through Sendai virus reprogramming methods.” What level of automation is required/expected during the reprogramming and expansion process? Is the lack of automation a disqualifier?
The reprogramming and expansion phases should be fully automated. The lack of automation in the reprogramming methods is disqualifying. It will need to be fully automated to reduce variability between lines.
Do we need to use the kits and equipment specified in the RFP for the QC assays or are similar alternatives acceptable?
Yes, the kits and equipment specified in the Statement of Work shall be used.
3 What matrix will be used to culture the cells? Feeder-free or MEF feeder dependent? Feeder-free.
Given the 6-9 month timeline provided following receipt of the blood or PBMCs, is the expectation that the up to 200 lines will be completed in the two-year contract period or that all will have been started?
Yes, all 200 lines shall be completed in the two-year contract period.
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